@article {1369, title = {GC-MS Analysis and Screening of Anti-Proliferative Potential of Methanolic Extract of Garcinia cowa on Different Cancer Cell Lines}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {March 2021}, pages = {347-361}, type = {Original Article}, chapter = {347}, abstract = {

Introduction: Garcinia cowa (Clusiaceae) is popular among integrative medicine in several communities. This study undertook to evaluate the anti-proliferative activity on cancer cells and its cytotoxic effect on normal cells. Here we are reporting for the first time the metabolomic profiling of G. cowa leaf. Methods: Anti-proliferative potential of ethyl acetate and methanol extract of Garcinia cowa leaf assessed by MTT assay. Metabolomic profiling obtained by GC/ MS analysis. Nuclear morphology visualized by DAPI staining. Caspase activation analysed through spectrophotometric assay. Results: The study reveals, that the methanolic extract is more potential in inducing anti-proliferative activity than ethyl acetate extract. Robust antiproliferative activity of the methanolic extract evidenced in lung cancer cell line, A549 followed by MCF{\textendash}7, HepG2, MOLT {\textendash} 4, MDA-MB-468 cells. The anti-proliferative effect was negligible in normal PBMC. Further, a dose-dependent increase of nuclear fragmentation visualized in A549 cells treated with the methanolic extract. Post methanolic extract treatment upregulation of caspase-3 and caspase-9 also evidenced in A549 cells. GC/MS analysis revealed the presence of phytoconstituents of different phytochemical groups comprising of 3.45\% diterpenoid, 5.45\% triterpenoid, 11.24\% steroid, 2.03\% phytosterol, etc. in methanol extract, as well as 4.53\% diterpenoid, 2.88\% triterpenoid, 1.09\% steroid, 2.11\% phytosterol, etc. in ethyl acetate extract with considerable biological importance. Conclusion: This is the maiden report of the metabolomic profiling of leaf extracts of Garcinia cowa which possess a good repository of potentially bioactive molecules that holds a great promise as a future therapeutic agent in combating lung cancer.

}, keywords = {Anti-proliferative, Cancer, Garcinia cowa, GC-MS, Metabolomic profiling}, doi = {10.5530/pj.2021.13.45}, author = {Anirban Chouni and Amrita Pal and Priya K Gopal and Santanu Paul} } @article {1147, title = {Screening Data Reveals that Spirogyra triplicata, a Fresh Water Algae Induces Robust Anti-Proliferative Activity Against A549 Cells}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {569-577}, type = {Research Article}, chapter = {569}, abstract = {

Introduction: Algae comprise a promising source of novel components with potent therapeutic agents. In particular, algae have been considered as a potential source of new bioactive compounds. The antioxidant data of our previous study with six different algal methanolic extract reveals the presence of high antioxidant, total phenol content and total flavonoid content in Spirogyra triplicata. Thus, we further focused on screening the anti-proliferative activity of six different green algae on five different cancer cell lines like MCF7, A549, HEPG2, REH, MOLT4. Methods: To fulfill our aim we performed MTT assay for testing anti-proliferative activity and DAPI staining for observing nuclear morphology. We also looked into the metabolomic profiling of Spirogyra triplicata by GC-MS chemometric study. Results: The result indicates that after 24 hours of treatment with methanolic extract of Spirogyra triplicata A549 was the most sensitive cell line with IC50 value of 24.07 {\textpm} 1.09 μg/ml. Followed by Rhizoclonium fontinale and Hydrodictyon reticulatum with IC50 value of 25.97 {\textpm} 1.94 μg/ml and 32.50 {\textpm} 1.97 μg/ml respectively. The HEPG2 cell line was the second most sensitive cell line against S. triplicata with IC50 value of 30.20 {\textpm} 1.45 μg/ml. The MOLT4 cell line was detected as most resistant cell line against the green algal extract in this study. Though the methanolic extracts of six green algae showed maximum to moderate anti-proliferative activity on different cancer cell line but no significantly affect on normal PBMC was observed. Nuclear fragmentation was observed in a dose dependent fashion by DAPI staining on A549 cells treated with methanolic extract of Spirogyra triplicata. We further looked into the chemo profiling of Spirogyra triplicata by GCMS analysis. The result of GC-MS clearly indicates presence of nineteen major components and twenty-three minor components which have more or less bioactivity and would help in therapeutics in future. Conclusions: In brief this study indicates for the first time that green algae Spirogyra triplicata induces anti-proliferative activity specifically against A549 cell but not in normal PBMC. It can be concluded that Spirogyra triplicata holds a great promise as a good repository of anti cancer compounds which may be used in future drug discovery.

}, keywords = {A549, Anti-proliferative, GCMS, Spirogyra triplicata}, doi = {10.5530/pj.2020.12.86 }, author = {Ankita Mridha and Priya K Gopal and Santanu Paul} } @article {783, title = {A Comparative Antibacterial Activity of Three Common Spices Extract and their Anti-Proliferative and Apoptotic Effectiveness against Human Breast Adenocarcinoma Cells}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {January 2019}, pages = {88-93}, type = {Original Article}, chapter = {88}, abstract = {

Objective: The present study was performed to analyse the antibacterial potential as well as the anti-proliferative and apoptotic efficacy of three common spices viz. Cardamom (Elettaria cardamomum), Cinnamon (Cinnamomum verum) and Fennel (Foeniculum vulgare). Methods: Antibacterial activity was determined by well diffusion assay against selected bacterial strains. Anti-proliferative activity was evaluated by cell viability assay and the apoptotic effect was observed by nuclear fragmentation analysis in MCF-7 cells. Results: The antibacterial activity result revealed that Cinnamon extract (CIE) showed maximum antibacterial activity against selected test organism followed by Cardamom (CAE) and Fennel (FEE). The cell viability results revealed that FEE induces the highest cytotoxicity (IC50 73.9 μg/ml) against MCF-7 cells, while CIE showed the lowest efficacy (IC50 98.2 μg/ml) as compared to control. Conclusion: The findings revealed that CIE has the most potent antibacterial efficacy, whereas FEE was found to be a more potent anti-proliferative and apoptotic agent against human breast carcinoma MCF-7 cells.\ 

}, keywords = {Anti-proliferative, Antibacterial, Apoptotic, Cell viability, Nuclear fragmentation}, doi = {10.5530/pj.2019.1.16}, author = {Shabana Bano and Asif Jafri and Nashrah Ahmad and AK Sharma and Md Arshad} }