@article {1830, title = {Effect of Seahorse Extract (Hippocampus comes L.) on Caspase-3 and TUNEL assay in Rats After Depot Medroxyprogesterone Acetate Induction}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {253-258}, type = {Original Article }, chapter = {253}, abstract = {

Seahorse (Hippocampus spp) is marine fish and have pharmacological activity, such as an antiinflammatory, antioxidative, antifatigue and improve the fertility. Depot medroxyprogesterone acetate (DMPA) is a contraception drug for male and affect the endocrine system by inhibiting pituitary gonadotropin with reduce testosterone levels in 12 weeks. There are limited studies reported the effects seahorse extract (SE) on Caspase-3 and TUNEL assay in rats induced by DMPA. Thirty Sprague-Dawley (SD) male rats that were induced by 1.25mg/kgbw DMPA in 0 and 12 weeks. The animals were randomly into five groups, following: aquadest (G1), CMC 1\% (G2), SE dose of 150 mg/kgbw (G3), SE dose of 225 mg/kgbw (G4), SE dose of 300 mg/kgbw (G5). The rats were gavage every day from seven until week eighteen. On the last week, we taken the right and left testis to observed the apoptotic on Caspase-3 and TUNEL assay. Apoptotic marker was observed through immunohistochemistry from testicular tissue and analysed with plugin ImageJ IHC profiler, which is H-score as the results. Data were analysed using One-Way ANOVA and Bonferroni{\textquoteright}s post hoc tests. The SE decrease the Caspase-3 and TUNEL assay expression in rats induced by DMPA until eighteen weeks, with dose 150 mg/kgbw given the significant difference with p=0.028; \<0.05 and p=0.000; \<0.01. These results suggest that SE decreased germ cells apoptotic in DMPA induced rats.

}, keywords = {Apoptotic, Caspase-3, DMPA, Seahorse, TUNEL assay}, doi = {10.5530/pj.2022.14.93}, author = {Trisnawati Mundijo and Yurnadi Hanafi Midoen and Franciscus D. Suyatna and Agung Eru Wibowo and Kusmardi Kusmardi} } @article {783, title = {A Comparative Antibacterial Activity of Three Common Spices Extract and their Anti-Proliferative and Apoptotic Effectiveness against Human Breast Adenocarcinoma Cells}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {January 2019}, pages = {88-93}, type = {Original Article}, chapter = {88}, abstract = {

Objective: The present study was performed to analyse the antibacterial potential as well as the anti-proliferative and apoptotic efficacy of three common spices viz. Cardamom (Elettaria cardamomum), Cinnamon (Cinnamomum verum) and Fennel (Foeniculum vulgare). Methods: Antibacterial activity was determined by well diffusion assay against selected bacterial strains. Anti-proliferative activity was evaluated by cell viability assay and the apoptotic effect was observed by nuclear fragmentation analysis in MCF-7 cells. Results: The antibacterial activity result revealed that Cinnamon extract (CIE) showed maximum antibacterial activity against selected test organism followed by Cardamom (CAE) and Fennel (FEE). The cell viability results revealed that FEE induces the highest cytotoxicity (IC50 73.9 μg/ml) against MCF-7 cells, while CIE showed the lowest efficacy (IC50 98.2 μg/ml) as compared to control. Conclusion: The findings revealed that CIE has the most potent antibacterial efficacy, whereas FEE was found to be a more potent anti-proliferative and apoptotic agent against human breast carcinoma MCF-7 cells.\ 

}, keywords = {Anti-proliferative, Antibacterial, Apoptotic, Cell viability, Nuclear fragmentation}, doi = {10.5530/pj.2019.1.16}, author = {Shabana Bano and Asif Jafri and Nashrah Ahmad and AK Sharma and Md Arshad} } @article {871, title = {Virtual Screening of Indonesian Herbal Database for Discovery of Procaspase-3 Activators Using Autodock and Autodock Vina}, journal = {Pharmacognosy Journal}, volume = {xx}, year = {2019}, pages = {xx-xx}, type = {Original Article}, chapter = {xx}, abstract = {

Objective: Cancer is a disease where body cell grows abnormal, spread to every part of human body. Previous studies have found excessive expression of Procaspase-3 on cancer that must be activated to Caspase-3 to induce apoptotic in cells. Methods: Virtual screening of Indonesian Herbal Database was carried out to discover Procaspase-3 activators. This study was validated using enrichment factor (EF), receiver operating characteristics (ROC) area under curve (AUC) parameters. Among 1412 compounds were screened using Autodock and Autodock Vina software. Results: The virtual screening results using Autodock obtained the best ten compounds with binding energy -8.28 ~ -9.31 kcal/mol and Autodock Vina obtained the best ten compounds with binding energy -8.1 ~ -8.8 kcal/mol. Both virtual screening software showed two compounds in common, i.e., betulinic acid and maslinic acid. Conclusion: Betulinic acid interacts with Leu136A, Lys137A, Tyr195A and Pro201 residues in Autodock and Autodock Vina. While maslinic acid interacts with Leu136A, Lys137A and Pro201 residues in Autodock and Autodock Vina.

}, keywords = {Apoptotic, Cancer, Herbal, Procaspase-3 activator, Virtual Screening}, doi = {10.5530/pj.2019.11xx}, author = {Rezi Riadhi Syahdi and Ayu Annissa and Arry Yanuar} } @article {839, title = {Virtual Screening of Indonesian Herbal Database for Discovery of Procaspase-3 Activators Using Autodock and Autodock Vina}, journal = {Pharmacognosy Journal}, year = {2019}, pages = {xx-xx}, type = {Original Article}, chapter = {xx}, abstract = {

Objective: Cancer is a disease where body cell grows abnormal, spread to every part of human body. Previous studies have found excessive expression of Procaspase-3 on cancer that must be activated to Caspase-3 to induce apoptotic in cells. Methods: Virtual screening of Indonesian Herbal Database was carried out to discover Procaspase-3 activators. This study was validated using enrichment factor (EF), receiver operating characteristics (ROC) area under curve (AUC) parameters. Among 1412 compounds were screened using Autodock and Autodock Vina software. Results: The virtual screening results using Autodock obtained the best ten compounds with binding energy -8.28 ~ -9.31 kcal/mol and Autodock Vina obtained the best ten compounds with binding energy -8.1 ~ -8.8 kcal/mol. Both virtual screening software showed two compounds in common, i.e., betulinic acid and maslinic acid. Conclusion: Betulinic acid interacts with Leu136A, Lys137A, Tyr195A and Pro201 residues in Autodock and Autodock Vina. While maslinic acid interacts with Leu136A, Lys137A and Pro201 residues in Autodock and Autodock Vina

}, keywords = {Apoptotic, Cancer, Herbal, Procaspase-3 activator, Virtual Screening}, doi = {10.5530/pj.2017.4.x}, author = {Rezi Riadhi Syahdi, and Ayu Annissa and Arry Yanuar} }