@article {1424, title = {Evaluation of Acute toxicity, In-vitro, In-vivo Antidiabetic Potential of the Flavonoid Fraction of the plant Chenopodium album L}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {May 2021}, pages = {765-779}, type = {Research Article}, chapter = {765}, abstract = {

Background: The Chenopodium album L. commonly recognized as Bathua, is widely distributed globally and contains various phytoconstituents that help treat several diseases. However, until now, aerial parts{\textquoteright} antidiabetic potential and the plant{\textquoteright}s acute toxicity at fraction level have never been established. Objectives: To investigate the acute toxicity, the in-vitro, in-vivo antidiabetic potential of the plant at fraction level. Materials and Methods: The aerial parts of the plant were fractionated into different fractions, i.e., flavonoid fraction (CAFF), tannin fraction (CATF), alkaloid fraction (CAAF), saponin fraction (CASF), and were analyzed for in-vitro alpha-amylase inhibition assay. The CAFF, CATF, and CAAF were selected based on in-vitro alpha-amylase inhibition assay results and were further screened for its acute toxicity and in vivo antidiabetic activity using a high-fat diet and streptozotocin-induced diabetes model. The CAFF was characterized by LC-MS, and a molecular docking study was carried out. Results: The in-vitro alpha-amylase inhibition assay revealed that CAFF was found to be more potent than standard Acarbose having IC50 values 122.18 {\textpm} 1.15 and 812.83{\textpm} 1.07 μg/ml, respectively. The CAFF fraction was found to possess potent antidiabetic activity in a dose-dependent manner in both in vitro and in vivo diabetic models and did not produce any sign of severe toxicity. Furthermore, the bioactive CAFF fraction was characterized by LC-MS, showed the presence of quercetin 3-O-(2{\textquoteright}{\textquoteright},6{\textquoteright}{\textquoteright}-di-O-rhamnosyl) glucoside (QRG) or quercetin 3-O-(2{\textquoteright}{\textquoteright},6{\textquoteright}{\textquoteright}-di-Orhamnosyl) galactoside (QRGa) and quercetin 3-O-rutinoside (rutin) (QR). It is predicted from the molecular docking study that the CAFF fraction primarily acts as an alphaamylase inhibitor. Conclusion: The CAFF fraction was found to poses dose-dependent potent antidiabetic activity and did not produce any sign of severe toxicity and primarily act as an alpha-amylase inhibitor.

}, keywords = {Acute toxicity, Alpha-amylase, Antidiabetic activity, Chenopodium album, Lc-Ms, Molecular docking}, doi = {10.5530/pj.2021.13.98}, author = {Neeraj Choudhary and Pranav Kumar Prabhakar and Gopal L Khatik and Subba Rao Chamakuri and Devesh Tewari and Ashish Suttee} } @article {909, title = {Studies on an in-vitro Investigation of Anti Diabetic Property of a Hemiparasitic Taxa Dendrophthoe falcata (L.f.) Ettingsh (Loranthaceae)}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {July 2019}, pages = {699-704}, type = {Original Article}, chapter = {699}, abstract = {

Dendrophthoe falcata (L.f.) Ettingsh, belong to the family Loranthaceae an unique hemiparasitic taxon, spreads almost all the entire forest in South West Bengal. It is generally stem hemi parasitic taxa and habitat mostly on aerial part of host plant having strong in spite of common root system. It is depend on the host plant for mainly water and to some extend carbon. It synthesizes its own food. The haustoria have penetrated to the vascular bundle mainly in the xylem tissue of the host plants. It has numerous medicinal values with a long history of its use in Chinese traditional medicines. Objectives: The present work deals with the anti-diabetic property medicinal aspect. Methods: The leaves extract mixed with the amylase enzyme then add the starch solution and allow the reaction for few minutes after that the reaction mixture interrupted with DNS. Results: The responsible enzyme i.e. Alpha-amylase breaks the large starch molecules which produces free glucose and simultaneously increases the blood sugar level and as a result hyperglycemia occurs. In this experiment increase the concentration of leaves extract which has decreased the activity of this potent enzyme. Conclusions: This study also reveals that, the plant{\textquoteright}s leaves extracts have inhibitory activity on this key enzyme viz. alpha amylase which is very crucial data for drug preparation and other research purposes.

}, keywords = {Alpha-amylase, Dendrophthoe falcata, Loranthaceae, Medicinal value}, doi = {10.5530/pj.2019.11.111}, author = {Ayan Kumar Naskar and Souradut Ray and Sanjukta Mondal Parui and Amal Kumar Mondal} } @article {79, title = {Preliminary Phytochemical analysis and In vitro Antioxidant, FTIR Spectroscopy, Anti-diabetic activity of Acacia catechu ethanolic seed extract}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {Nov-Dec 2015}, pages = {356-362}, type = {Original Article}, chapter = {356}, abstract = {

Objective: To evaluate the preliminary phytochemical analysis and in vitro antioxidant activity, anti-diabetic effect of ethanolic seed extract of Acacia catechu against the alpha amylase and alpha glucosidase digestive enzymes in the pancreas and small intestine. Methods: Preliminary phytochemical analysis was done by adopting the method of Evans. Antioxidant assay is performed by DPPH, ABTS and FRAP assay, Anti diabetic activity was determined by modified method of miller, the extract at different concentrations was tested for mammalian alpha amylase and alpha glucosidase enzyme inhibitory assay under the controlled experimental conditions and subjected to determination of absorbance. Results: The present study reveals the presence of few secondary metabolites and the extract exhibits potent Antioxidant activity and a concentration dependent inhibition of Alpha amylase and Alpha glucosidase. Conclusion: From the present study it can be concluded that ethanolic seed extract of Acacia catechu possessed marked in vitro antioxidant and anti-diabetic effect. The effect was plausibly due to the presence of phenolic contents of Acacia catechu

}, keywords = {Acacia catechu seed, Alpha glucosidase, Alpha-amylase, Antidiabetic, Antioxidant, FTIR Spectroscopy, Phytochemical}, doi = {10.5530/pj.2015.6.7}, author = {Thangavelu Lakshmi and Rajendran Ramasamy and Rathinam Thirumalaikumaran} }