@article {171, title = {Phytochemical and In vitro Antidiabetic Activity of Psidium Guajava Leaves}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {June/2016}, pages = {392-394}, type = {Original Article}, chapter = {392}, abstract = {

Objective: The present investigation includes the preliminary screening of phytochemicals and evaluation of in vitro antidiabetic activity of Psidium guajava leaves extracts. Materials and Methods: Plant material was subjected to the extraction preparation by soxhlet apparatus by using various solvents such as aqueous, ethanol, chloroform, petroleum ether and hexane. The various kinds of phytochemicals were detected and then in vitro antidiabetic activity of P. guajava were detected by using alpha amylase and alpha glucosidase enzyme in an in vitro model. Results: The study reveals the presence of phytochemicals such as carbohydrate, tannin, flavonoids, phenols etc., Among the various extracts the aqueous and ethanolic extracts which contains the large number of phytoconstituents. The P. guajava leaves has been successfully inhibited both the enzymes in an in vitro model. The aqueous extracts of P. guajava leaves inhibited the alpha amylase and alpha glucosidase enzymes as 72.1\% and 74.8\% respectively. The ethanolic extract of P. guajava leaves inhibited the alpha amylase and alpha glucosidase enzymes as 97.5\% and 91.8\% respectively. Conclusion: From the results obtained in the current studies, the P. guajava leaves have a prominent antidiabetic property in an in vitro model and further studies can be carried out in an in vivo model and the isolation of activie compound from P. guajava leaves extract is needed.

}, keywords = {Alpha amylase, Alpha glucosidase, P. guajava, Phytochemical.}, doi = {10.5530/pj.2016.4.13}, author = {Ramasamy Manikandan and Arumugam Vijaya Anand and Sampath Kumar and Pushpa} } @article {79, title = {Preliminary Phytochemical analysis and In vitro Antioxidant, FTIR Spectroscopy, Anti-diabetic activity of Acacia catechu ethanolic seed extract}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {Nov-Dec 2015}, pages = {356-362}, type = {Original Article}, chapter = {356}, abstract = {

Objective: To evaluate the preliminary phytochemical analysis and in vitro antioxidant activity, anti-diabetic effect of ethanolic seed extract of Acacia catechu against the alpha amylase and alpha glucosidase digestive enzymes in the pancreas and small intestine. Methods: Preliminary phytochemical analysis was done by adopting the method of Evans. Antioxidant assay is performed by DPPH, ABTS and FRAP assay, Anti diabetic activity was determined by modified method of miller, the extract at different concentrations was tested for mammalian alpha amylase and alpha glucosidase enzyme inhibitory assay under the controlled experimental conditions and subjected to determination of absorbance. Results: The present study reveals the presence of few secondary metabolites and the extract exhibits potent Antioxidant activity and a concentration dependent inhibition of Alpha amylase and Alpha glucosidase. Conclusion: From the present study it can be concluded that ethanolic seed extract of Acacia catechu possessed marked in vitro antioxidant and anti-diabetic effect. The effect was plausibly due to the presence of phenolic contents of Acacia catechu

}, keywords = {Acacia catechu seed, Alpha glucosidase, Alpha-amylase, Antidiabetic, Antioxidant, FTIR Spectroscopy, Phytochemical}, doi = {10.5530/pj.2015.6.7}, author = {Thangavelu Lakshmi and Rajendran Ramasamy and Rathinam Thirumalaikumaran} }