@article {1076, title = {Qualitative and Quantitative Assay of Hydroxycinnamates of Prunus spinosa L.}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {February 2020}, pages = {157-161}, type = {Research Article}, chapter = {157}, abstract = {

Background: Blackthorn (Prunus spinosa L.) is a plant commonly found in the Russian Federation on the roadsides, forest margins, and meadows. Despite lack of recognition by the official medicine, blackthorn fruits possess antioxidant properties and are used in homeopathic preparations. They may also demonstrate antibacterial and anticancer potential due to hydroxycinnamic acids. The aim of present study was to identify and assay hydroxycinnamates in fruits of P. spinosa cultivated in Moscow Region. Materials and Methods: Fresh and dried fruits of P. spinosa, gathered from plants cultivated in Moscow Region in the harvest maturity stage, were used in the study. Qualitative composition of hydroxycinnamic acids was assessed by high performance liquid chromatography, using reversed phase C18 column. Total hydroxycinnamic acids (THA) content was assessed spectrophotometrically. Results: Similar chromatographic profiles were obtained for both fresh and dried blackthorn fruits, the two most abundant compounds being epicatechin (2.91\%) and chicoric acid (2.90\%). Fruits gathered in Chekhovsky District had lower content of hydroxycinnamates (0.798 {\textpm} 0.89) than those coming from Klinsky District (0.886 {\textpm} 0.92). However, the THA content in dried fruits grown in both districts was found to be similar (0.540 {\textpm} 0.71 and 0.557 {\textpm} 0.74, respectively). Conclusions: It can be concluded that blackthorn fruits can be considered as a source of hydroxycinnamic acids, as both fresh and dried fruits contain at least eleven hydroxycinnamates. It was found that the dried fruits have similar content of hydroxycinnamic acids, independently of their origin. Future research should be aimed at drying method optimization.

}, keywords = {Blackthorn, Herbal raw material, HPLC, Hydroxycinnamic acids, Prunus Spinosa L., Spectrophotometry}, doi = {10.5530/pj.2020.12.23}, author = {Sergey Kondrashev and Nadezhda Nesterova and Alexey Luzin and Vitaliy Kochanov and Anna Luzina and Alexey Matyushin} } @article {829, title = {HPLC Quantification of Hydroxycinnamic and Organic Acids of Canadian Goldenrod (Solidago canadensis L.)}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {February 2019}, pages = {400-404}, type = {Original Article}, chapter = {400}, abstract = {

Background: Canadian goldenrod (Solidago canadensis L.) is a medicinal plant widely used in traditional medicine across the world for several hundred years. According to literature data, S. canadensis contains various groups of biologically active substances, including tannins, flavonoids, etc. The aim of the study was to identify and quantify hydroxycinnamic and organic acids in aerial parts of Canadian goldenrod, as these groups of substances demonstrate a broad spectrum of therapeutic activities. Materials and Methods: Ethanolic extracts of S. canadensis, gathered in Central Russia, were analyzed using highperformance liquid chromatography (HPLC). Hydroxycinnamic acids (HCA) determination was carried out by HPLC method with UV detection at 330 nm using HCA reference standards (RS). Organic acids (OA) determination was performed in the same manner, utilizing UV detection at 210 nm and corresponding OA RS. Results: It was established that S. canadensis HCA composition is represented by cichoric, caffeic, chlorogenic, quinic and ferulic acids. The total HCA content in was 1.16 g {\textpm} 10.7 mg / 100 g. Main OA, found in S. canadensis, are ascorbic, citric, tartaric, succinic, gallic, malic, oxalic and fumaric acids, with the total OA content of 426.5 mg {\textpm} 6.4 mg / 100 g. Conclusion: The described HPLC method was successfully used for analysis of S. canadensis aerial parts ethanolic extracts. The method can be utilized for HCA and OA identification and quantification in both herbal raw material and herbal medicinal products containing Canadian goldenrod.

}, keywords = {Canadian goldenrod, HPLC, Hydroxycinnamic acids, Organic acids, Solidago canadensis}, doi = {10.5530/pj.2019.11.62}, author = {Fidan Suleymanova and Olga Nesterova and Alexey Matyushin} }