@article {2491, title = {Mucuna Pruriens Seeds Extract Loaded Phytosomal Intranasal Gel for the Effective Treatment of Parkinson{\textquoteright}s Disease}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {April 2025}, pages = {129-154}, type = {Original Article}, chapter = {129}, abstract = {

One phytoconstituent derived from Mucuna pruriens (legumes) is levodopa. This medication{\textquoteright}s oral usage is limited by its high first-pass metabolism and low absorption. The goal of the present research was to develop a phytosomal gel filled with levodopa extract for better delivery and brain targeting. Various techniques, including solvent evaporation, salting out anti-solvent precipitation, direct egg yolk, and egg lipids methods, were used to create phytosomal formulations. Scanning electron microscopy, particle size, x-ray diffraction, and other techniques were used to characterize phytosomes. And added into gel formation, the more successful batch was examined for several parameters. The final batch underwent a variety of animal tests, including pharmacokinetic analysis, irritation to the nasal cavity testing. The most effective phytosomes were those made via the antisolvent precipitation approach. In this investigation, a 32-randomized complete factorial design was employed. Batch F4 had an entrapment efficiency of 70\%, a particle size of 15 (μg) and 60\% CDR. The gel-formulated batch F4G3 demonstrated improved results in terms of extrudability (90.82), amount of drug (89.32\%), viscosity (5421 cps at 100 rpm), and spreadability (25.18). Batch F4G3 of the Mucuna pruriens phytosome gel exhibited Higuchi{\textquoteright}s kinetics. According to the findings of the animal study, dopamine levels were significantly elevated. The pharmacokinetic and nasal irritation studies showed notable in vitro penetration of the nasal mucosa without resulting in skin irritation. For improving Parkinson{\textquoteright}s disease treatment, the phytosomal gel formulation delivered via the nasal route would be the ideal option.

}, keywords = {Gel, L-Dopa extract, Mucuna pruriens, Nasal gel, Optimization, Phytosome}, doi = {10.5530/pj.2025.17.18}, author = {Aniruddha Kulkarni and Manoj Tare and Meera Singh} } @article {987, title = {Phytosomal Nanoparticles Preparation of Curcuminoids to Enhance Cellular Uptake of Curcuminoids on Breast Cancer Cell Line MCF-7}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {September 2019}, pages = {1037-1045}, type = {Original Article}, chapter = {1037}, abstract = {

Objective: Curcuminoids, the bioactive compounds extracted from Curcuma longa consisting of Curcumin (CUR), demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC), have shown promising biological effects, including anticancer activity. This study sought to prepare a physically stable phytosomal nanoparticles of curcuminoids (Curs-Phyto) to facilitate uptake of curcuminoids on breast cancer cells line, and further increase the cytotoxicity against cancer cells. Methods: The evaporation combined extrusion technique was employed to prepare phytosomal curcuminoids nanoparticles. The interaction between curcuminoids and phospholipid by a hydrogen bond was confirmed by differential scanning calorimetry (DSC), powder X-ray diffraction (XRD), fourier transform infrared (FT-IR), and 1H nuclear magnetic resonance (1H-NMR). Their physicochemical characterizations and stability in simulated gastric and intestinal media were investigated. The effects of Curs-Phyto on MCF-7 cells were evaluated by flow cytometry, MTS assay and cell cycle analysis. Results: We found that the Curs-Phyto were formed at a spherical shape with good size (~ 180 nm), a narrow size distribution (PDI \< d0.2), high complexation rate (~ 87\%, 95\%, and 90\% for BDMC, DMC, and CUR respectively) and high loading capacity of curcuminoids. More importantly, the Curs-Phyto showed the increased cellular uptake and enhanced cytotoxicity against MCF- 7 cancer cells, compared to free curcuminoids. Conclusion: These results indicated that the phytosome could be a promising oral delivery system for curcuminoids for cancer treatment.

}, keywords = {Cellular uptake, Curcuminoids, FACS analysis, Nanoparticle, Phytosome}, doi = {10.5530/pj.2019.11.163}, author = {Nguyen Van Long and Bui Thi Thu Ha and Anh Vu Tuan and Hoang Van Luong and Nguyen Tung Linh and Thanh Chu Duc and Phung Cao Dai and Chul Soon Yong and Chu Van Men} }