@article {1019, title = {Influence of Milk on Phenolic Composition and Antioxidant Power of Black Tea}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2019}, pages = {1262-1268}, type = {Original Article}, chapter = {1262}, abstract = {

Introduction: Tea leaves are rich in several polyphenolics and flavonoidal compounds that could potentially have health-promoting properties. The objective of the present study was to analyze the antioxidant capacity of black tea and to study the effect of addition of fresh milk and packed milk on the phenolics concentrations and antioxidant efficacy. Methods: The black tea infusion (BT), black tea infusion with fresh milk (BTFM) \& black tea infusion with packed milk (BTPM) samples were comparatively analyzed for total phenolics, flavonoids and HPLC profiling of major phenolic content. All samples were investigated for their total antioxidant capacity and glutathione level in different organs (brain, liver, kidney and heart). Results: The results shown that BT(black tea infusion) had higher total phenolics and flavonoids followed by BTPM (black tea infusion with packed milk) and then the BTFM (black tea infusion with fresh milk). The analytical HPLC results obtained also indicated that BT contained higher amount of catechins and garlic acid derivatives than BTFM, and BTPM may be due to chelation of free phenolics with some fats and protein in the tested milk samples, which reduces the levels of free phenolics significantly. Concerning antioxidants capacity both tested milk samples reduced antioxidant capacity to more than 50\% in different tested tissues. Conclusion: We conclude that black tea is a valuable source of antioxidants and that the inhibitory effect of milk on the total antioxidant capacity may be related to complex formation of the fat \& protein content of the milk with major phenolics in the black tea.

}, keywords = {Antioxidant, Black tea, Glutathione, HPLC. Flavonoids, Milk, Phenolics}, doi = {10.5530/pj.2019.11.196}, author = {KM Meselhy and Ghada A Abdel-latif and Amany A sleem and Walaa Ayman and Maram K Imam and Kholoud A Kassab and Sherouk Eissa} } @article {1528, title = {Protective effect of cissampelos pareira linn. Extract on gentamicin-induced nephrotoxicity and oxidative damage in rats}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {26th May 2014}, pages = {59-67}, type = {Research Article}, abstract = {

Objective: To evaluate the potential nephroprotective and antioxidant activity of hydroalcoholic Cissampelos pareira (C. pareira) whole plant extract using gentamicin-induced rats. Methods: For studying acute toxicity study, single oral dose of 2g/kg hydroalcoholic extract of C. pareira was evaluated in rats by oral gavage. The nephroprotective activity was evaluated using gentamicin-induced model in rats. In-vitro antioxidant activity was evaluated by using DPPH assay and reducing power assay. In-vivo antioxidant activity was evaluated by using glutathione and lipid peroxidation estimations in gentamicin-induced rats. Hydroalcoholic C. pareira whole plant extract was given at a dose of 200 and 400 mg/kg p.o. Results: For acute toxicity testing rats administered with the extract at a dose 2 g/ kg. the result showed no toxicity. Hydroalcoholic C. Pareira whole plant extract (200 and 400 mg/kg p.o) significantly decrease the elevated urinary glucose levels in the urine, decrease the elevated urea and creatinine levels in blood and increase the urinary creatinine levels in gentamicin-induced nephrotoxic rats. The extract had shown significant dose dependent increase in the DPPH and reducing power activity. There were a dose dependent decreasing and increasing of lipid peroxidation, glutathione levels in hydoalcoholic extract treated groups respectively. Conclusion: This study exhibits that hydroalcoholic C. pareira whole plant extract poses nephroprotective activity which may be due to its antioxidant activity.

Key words: Gentamicin, Cissampelos pareira, creatinine, urea, glucose, lipid peroxidation, glutathione, reducing power, DPPH.

}, keywords = {Cissampelos pareira, Creatinine, DPPH, Gentamicin, Glucose, Glutathione, Lipid peroxidation, Reducing power, urea}, author = {D. R Ch Sekhara Reddy and G Siva Kumar and B. M. Vrushabendra Swamy and K. Phani Kumar} }