@article {2014, title = {Effect of Thiamine on Serum Glutamate in Ischemic Stroke Animal Model}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {April 2023}, pages = {390-392}, type = {Research Article}, chapter = {390}, abstract = {

Background: Thiamin or vitamin b1 is a therapy that has been widely used in neurology cases such as alcohol-induced encephalopathy and neuropathy. Thiamin has the ability to penetrate the blood brain barrier so that it is expected to be used as an additional therapy in stroke cases. Stroke is currently an emergency neurology case that causes high mortality and disability. Among the causes is because damage to large areas of the brain will cause the release of neurotransmitters such as glutamate which has excitotoxicity effects to the brain when excessive. Method: This study used animal models of carotid artery occlusion stroke. Divided into 4 groups: control, thiamin dose of 100mg/kg, Thiamin 200mg/kg, and 400mg/kg. Serum glutamate was collected on day 7 post-action. Then evaluated by ELISA method. Results: There was a significant difference in the form of a decrease in the average serum glutamate level between the control and the sample that received a dose of thiamine 200mg/kg and 400mg/kg. Conclusion: High-dose thiamine may be considered as an additional therapy in cases of carotid artery occlusion model stroke.

}, keywords = {Animal model, Glutamate, Ischemic, Stroke., Thiamine}, doi = {10.5530/pj.2023.15.60}, author = {Mohammad Saiful Ardhi and Muhammad Hamdan and Achmad Chusnu Romdhoni} } @article {1103, title = {Properties of A Thiamine Binding Protein Purified from Mung Bean}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {266-270}, type = {Original Article}, chapter = {266}, abstract = {

Thiamine (vitamin B1) was the first B vitamin which has been identified. It serves as a cofactor for several enzymes involved in energy metabolism. The laboratory test against thiamine deficiency can be done by measuring thiamine levels in the blood. The aim of this study was to identify the stability and the binding activity characters of TBP. The equilibrium dialysis technique was used to see the factors affecting the bond between TBP and thiamine. The MBTBP concentration of post-chromatographic affinity resulted from dilution of lyophilisate was stable for 30 days at -20{\textdegree}C and 3 days at 4{\textdegree}C. The optimal pH for binding MBTBP to thiamine was 7.5. Alkylation with iodoacetic acid decreased the binding capacity of TBP which suggested the presence of a{\textendash}SH or imidazol group in its active site. The importance of disulfide bridge was proven by decreasing of Thiamine binding capacity of TBP after β-mercaptoethanol treatment. This binding activity was also affected by oxidizing agents, but it was less affected by calcium ions and heavy metals.

}, keywords = {Binding capacity, Mung bean thiamine binding protein (MBTBP), Thiamine}, doi = {10.5530/pj.2020.12.41}, author = {Dwirini Retno Gunarti and Megawati Kartika and Mohamad Sadikin} }