@article {1121, title = {High Dose Allicin with Vitamin C Improves EPCs Migration from the Patient with Coronary Artery Disease}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {232-235}, type = {Original Article}, chapter = {232}, abstract = {

Endothelial Progenitor Cells (EPCs) have an important role in endothelial dysfunction repairment through neovasculogenesis and cardiac myocytes regeneration. However, EPCs migration is greatly reduced in the patient with Coronary Artery Disease (CAD). Allicin and Vitamin C are hypothesized to improve EPCs migration due to its antioxidant properties. Objective: To investigate the effect of Allicin and its combination with Vitamin C in EPCs migration of CAD patients. Material and Method: Mononuclear cells were isolated from CAD patients and cultured on fibronectin-coated plates with colony-forming unit Hill medium. The cells were divided into untreated (control), Allicin treatment (dose 100 mcg/ml, 200 mcg/ml, 400 mcg/ ml), and each dose of Allicin combined with 250 mcg/mL of Vitamin C. EPCs migration was assessed with Transwell Migration Assay Kit and evaluated by using statistical tests. Results: This research shows that EPC migration was significantly higher in the treatment. Allicin at all dose (dose 100 mcg/ml, 200 mcg/ml, 400 mcg/ml) and its combination with 250 mcg/mL of vitamin C compared to untreated group (p\<0.05). Allicin increase EPCs migration in a dosedependent manner. However, the only combination of 400 mcg/ml Allicin with 250 mcg/mL of vitamin C which has significantly higher EPCs migration compared to Allicin treatment alone. Conclusion: Allicin improves EPCs migration in a dose-dependent manner. Improvement of the migration only observed on the Allicin dose 400 mcg/ml with Vitamin C.

}, keywords = {Allicin, Antioxidant, Endothelial Progenitor, Migration}, doi = {10.5530/pj.2020.12.35}, author = {Yudi Her Oktaviono and Muhammad Rafdi Amadis and Makhyan Jibril Al-Farabi} } @article {1130, title = {Extracellular-Signal Regulated Kinase Signalling Pathway Mediates the Increased Proliferation of EPCs Treated with Garlic (Allium sativum) Extract, Purple Sweet Potato (Ipomoea batatas) Extract and Vitamin C}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2017}, month = {May 2020}, pages = {442-447}, type = {Original Article}, chapter = {442}, abstract = {

The endothelial progenitor cell (EPCs) proliferation capability is reduced in the patient with stable coronary artery disease (SCAD). Garlic (Allium sativum), purple sweet potato (Ipomoea batatas), and vitamin C are proven antioxidant which potentially improve EPCs proliferation ability. Objective: To investigate the effect of garlic (Allium sativum), purple sweet potato (Ipomoea batatas), and vitamin C in EPCs proliferation from CAD patients and identify the involvement of Extracellular-Signal Regulated Kinase (ERK) Signalling Pathway. Material and Method: Mononuclear cells were isolated from SCAD patients and cultivated with colony-forming unit (CFU)-Hill medium and divided into untreated (control), garlic extract (10 mcg/ml and 100 mcg/ml), purple sweet potato extract (1 mcg/ml and 25 μg/ml), and vitamin C (10 μg/ml and 250 μg/ml). EPCs proliferation was measured using the MTT Assay. Results: This research shows that EPCs proliferation was increased in the treatment with garlic extract at 10 mcg/ml and 100 mcg/ml dose (0.267 {\textpm} 0,003 and 0.391 {\textpm} 0.008 ; p \< 0.05), purple sweet potato extract at 1 mcg/ml and 25 μg/ml dose (0.250 {\textpm} 0.005 and 0.3562 {\textpm} 0.023 ; p \< 0.001), and vitamin C at 10 μg/ml and 250 μg/ml dose (0.259 {\textpm} 0.016 and 0.306 {\textpm} 0.022 ; p \< 0.001). Increased ERK expression was found in the treatment with garlic extract, purple sweet potato extract and vitamin C. Conclusion: Garlic extract, purple sweet potato extract, and vitamin C can increase EPC proliferation through the ERK signaling pathway.

}, keywords = {Antioxidant, Endothelial Progenitor, ERK, Proliferation}, doi = {10.5530/pj.2020.12.68}, author = {Yudi Her Oktaviono and Alisia Yuana Putri and Makhyan Jibril Al-Farabi and Yesita Rizky Firmansyah and Ferry Sandra} }