@article {1876, title = {Antiurolithiatic Activity of Aqueous Extract of Ziziphus lotus on Ethylene Glycol-Induced Lithiasis in Rats }, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {October 2022}, pages = {596-602}, type = {Research Article}, chapter = {596}, abstract = {

In Morocco, Ziziphus lotus is commonly used as an urolithiatic agent in the traditional medicine. To confirm this effect, an aqueous extract of Ziziphus lotus (AEZL) has been studied in ethylene glycolinduced urolithiasis model of rats. 30 male rats were randomly divided into five groups of six animals each. Group I served as a vehicle control and received distilled water (0.5 ml/100 g p.o.). All remaining groups received calculi inducing treatment for 28 days, comprised of 0.75\% v/v ethylene glycol with 1\% w/v ammonium chloride in drinking water ad libitum for 3 days followed by only 0.75\% v/v ethylene glycol for 25 days. Group II served as lithiatic control and received distilled water (0.5 ml/100 g p.o.). Group III served as curative treatment group and received AEZL at doses of 150 mg/kg from 14th day to 28th day. Group VI served as preventive treatment group and received AEZL at doses of 150 mg/kg from 1st day to 28th day. Group V served as therapeutic and received a drug {\textquotedblleft}Cystone{\textquotedblright} at dose of 750 mg/kg from 14th day to 28th day. The extract treatment decreased the levels of oxalate and calcium in urine. Crystalluria analysis showed that untreated rats excreted large CaOx monohydrate and few dihydrate crystals while treated animals excreted mostly small CaOx dihydrate crystals. Significant similarity was observed between preventive and therapeutic anti-urolithiatic effect of AEZL and anti-urolithiatic effect of cystone (P\<0.001). These results demonstrated that AEZL have an anti-urolithiatic effect with preventive and therapeutic treatments in this experimental condition.

}, keywords = {Ethylene glycol, Rats, Urinary parameter, Urolithiasis, Ziziphus lotus}, doi = {10.5530/pj.2022.14.141}, author = {Miloud Chakit and Rezklah Boussekkour and Aboubaker El Hessni and Youssef Bahbiti and Redouan Nakache and Hicham El Mustaphi and Abdelhalim Mesfioui} } @article {1244, title = {Antiurolithiatic Activity of Ethanolic Extract of Piper cubeba Dried Fruits: An in-vitro and in-vivo Study}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {September 2020}, pages = {1289-1296}, type = {Original Article}, chapter = {1289}, abstract = {

Introduction: Piper cubeba is a well-known traditional plant used in unani medicine belonging to the Piperaceae family and has been examined for the treatment of urolithiasis produced by calcium oxalate. Methods: Ethanolic extract of Piper cubeba (EEPC) dried fruits was subjected to phytochemical analysis and HPTLC fingerprinting. An in vitro antiurolithiatic analysis took place through conductometric titrations of CaCl2 with Na2C2O4. Acute toxicity studies conducted as per OECD guidelines. Urolithiasis was established in rats by supplementing 28 days with 0.75\% ethylene glycol in the ingesting water. Beside ethylene glycol, EEPC (100, 200 and 400 mg/kg) was given orally from 15 - 28 days, serum and urine were collected from individual animals and biochemical parameters like BUN, creatinine along with uric acid in serum as well as calcium, oxalate and phosphate in urine the kidney homogenate have been measured on 28th day. Kidney sections have been organized and histopathologically tested for calcium oxalate crystals. Results: Phytochemical analysis of EEPC disclose the presence of phenolics, tannins, steroids, terpenoids and flavonoids and HPTLC fingerprinting shows the presence of 7 terpenoids, 2 flavonoids when scanned at 540nm and 366nm. In vitro studies showed reduction in CaOx crystal aggregation and promoted nucleation after treatment with EEPC. In vivo studies also showed reduction in elevated levels of serum creatinine, BUN, uric acid, and levels of calcium, oxalate and phosphate in urine and kidney homogenate as compared to disease control rats. The results were supported by histopathological studies. Conclusion: The EEPC have shown significant antiurolithiatic activity by reducing calculi.

}, keywords = {calcium oxalate, Ethylene glycol, Flavonoids, HPTLC, Terpenoids, Urolithiasis}, doi = {10.5530/pj.2020.12.177}, author = {S Suman and SV Suresh Kumar} }