@article {2079, title = {Effect of Naphthaleneacetic Acid (NAA) and 6-Benzylamino Purine (BAP) on In-Vitro Propagation of "Mashua" (Tropaeolum tuberosum Ru{\'\i}z \& Pav{\'o}n) Morphotypes from Peru}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {August 2023}, pages = {563-568}, type = {Original Article }, chapter = {563}, abstract = {

Background: Tropaeolum tuberosum Ru{\i}z \& Pav{\'o}n {\textquotedblleft}mashua{\textquotedblright} is a native species of the central Andes and different propagation methods in vitro have been evaluated. However, this research has studied the in vitro propagation of 15 mashua (Tropaeolum tuberosum Ru{\'\i}z \& Pav{\'o}n) morphotypes from the Ayacucho and Apur{\'\i}mac regions (Peru) Objective: To evaluate the effect of the additives naphthaleneacetic acid (NAA) and 6-benzylamino purine (BAP) on the micro-propagation rate of T. tuberosum. Material and Methods: For in vitro establishment, seedlings were used after disinfection with sodium hypochlorite, propagated in Murashige and Skoog (MS) medium. For the evaluation of the effect of NAA and BAP additives, nodes were isolated from in vitro seedlings after 30 days of cultivation and transferred to MS medium supplemented with 3\% sucrose, pH 5.6 and 7 g/L of agar according to T1 (MS + NAA 1ppm) and T2 (MS + BAP 1ppm) treatments. The samples were cultivated for 28 days at 19 {\textdegree}C {\textpm} 2 {\textdegree}C with photoperiods of 16 h/light and 8 h/darkness. Results: After evaluating 20 repetitions for each of the three treatments, the addition of NAA or BAP does not improve the micropropagation rate of practically all the morphotypes studied. Furthermore, BAP behaved as an inhibitor of the development of mashua seedlings. Conclusion: The best micropropagation medium of Tropaeolum tuberosum Ru{\'\i}z \& Pav{\'o}n (mashua) is the basic Murashige and Skoog (MS) medium without the NAA or BAP additives.

}, keywords = {BAP., Mashua, Micropropagation, NAA, Tropaeolum tuberosum}, doi = {10.5530/pj.2023.15.120}, author = {Gilmar Pe{\~n}a-Rojas and Luz Quispe-Calle and Vidalina And{\'\i}a-Ayme and Alex Pereda-Medina and Rolando Estrada- Jimenez and Josefa Bertha Pari-Olarte and Elizabeth Julia Melgar-Merino and Jos{\'e} Santiago Almeida-Galindo and Oscar Herrera-Calderon} } @article {1289, title = {In vitro Propagation and Determination of Total Phenolic Compounds, Flavonoid Contents and Antioxidative Activity of Globba globulifera Gagnep}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {November 2020}, pages = {1740-1747}, type = {Research Article}, chapter = {1740}, abstract = {

Introduction: Currently, there is a reduction in the number of Globba globulifera, which is due to its slow multiplication rate, high susceptibility to pathogenic diseases and overexploitation of the plant from natural sources. In vitro culture to study suitable concentrations of plant growth regulators for shoot and root induction of G. globulifera. Bioactive compounds were measured by TPC, TFC and FRSA methods for comparison of those from in vitro and natural conditions. Methods: Microshoots were cultured on solid and liquid MS medium supplemented with various concentrations of cytokinins (BA, Kinetin and TDZ) and auxins (NAA and IBA) for eight weeks. Methanol was used as the extraction solvent via the ultrasonic method, TPC and TFC were both measured. DPPH for free radical scavenging activity was investigated. Results: The best result for shoot formation was achieved when culturing on MS medium with 3 mg/l and 5 mg/l of BAP or 5 mg/l of BAP plus 1 mg/l of IBA. The plantlets were transplanted to pots in a greenhouse. All the planting material showed a 100\% survival rate. The rhizomes of in vitro derived plantlets showed the highest value of TPC (52.28 mg GAE/g crude extract) and FRSA (93.55\%) and lowest of IC50 (0.46 mg/ml). Conclusion: The in vitro culture and antioxidant activity analysis could be the foundation for plant propagation in large quantities and the use of medicine.

}, keywords = {Bioactive compound, DPPH, Micropropagation, TFC, TPC, Zingiberaceae}, doi = {10.5530/pj.2020.12.236}, author = {Wipa Yaowachai and Surapon Saensouk and Piyaporn Saensouk} }