@article {1324, title = {Tinospora Sinensis (Lour.) Merr. Stem Modulate The TNF-Alpha Expression In HCT- 116 Tumour Cell, Besides the Inhibitory Effect on Cervical, Colon and Breast Cancer Cell Lines and Mycobacterium Tuberculosis H37Rv}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {January 2021}, pages = {8-16}, type = {Original Article}, chapter = {8}, abstract = {

Background: The present study was designed to evaluate TNF-Alpha experession, anticancer and antitubercular properties for the stem extracts of Tinospora sinensis (TS). Objective: natural product research is widely used for identifying hit molecules for life threatening diseases including cancer, tuberculosis and drug resistant infections. Materials and Methods: There were three polarity dependant solvent extracts obtained through cold maceration process using ethanol (ELTS), ethyl acetate (EATS) and n-hexane (NHTS), respectively. The extracts were subjected to MTT assay for their anticancer potential against HeLa (cervical cancer), MCF-7 (breast cancer) and HCT116 (colon cancer) cell lines, and based on the results, NHTS was subjected to flow cytometry for TNF-Alpha expression in HCT-116 cells. The antitubercular activity for the extracts was performed against Mycobacterium tuberculosis H37Rv (Mtb) by luciferase reporter phage (LPS) assay method. Results: The result of anticancer screening revealed that n-hexane extracts showed the significant inhibition (p\<0.05) on HCT-116 cells with the IC50 of 177.4 μg/ml, whereas EATS and ELTS were equally active on HeLa with the respective IC50 of 236 and 277 μg/ml. The NHTS was significantly effective on decreasing (P\<0.05) TNF-Alpha expression (31.27 MFU) in HCT-116 cells and is closely active with standard simvastatin (26.7 MFU) against the control (7.06 MFU). The antitubercular activity results revealed the equi-potency of both NHTS and EATS on Mtb with growth inhibition of 84 \% at 100μg/ml. The GC-MS analyses of NHTS confirmed the presence of Berberine, palmatine, tembertarine, magniflorine, choline and tinosporin. Conclusion: Overall, we scientifically support the traditional use Tinospora sinensis stem in the treatment of cancer and immune diseases.

}, keywords = {Anticancer, Antitubercular, HCT-116, Immunomodulatory, Tinospora sinensis, TNF-Alpha}, doi = {10.5530/pj.2021.13.2}, author = {Sreelakshmi Bada Venkatappa Gari and Ramalingam Peraman} } @article {1531, title = {Sterols and triterpenes from the non-polar antitubercular fraction of Abutilon indicum}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {26th May 2014}, pages = {49-52}, type = {Research Article}, abstract = {

Introduction:Abutilon indicum Sweet (Malvaceae) is used in Filipino folk medicine as demulcent, diuretic, sedative, aphrodisiac and antidiabetic remedy. It is also effective in the treatment of leprosy. It is also used as a laxative for patients having hemorrhoids and in the treatment of coughs, puerperal disease, urinary disorders, chronic dysentery and fever. Methods: Air-dried powdered leaves of A. indicum were initially extracted with DCM-methanol (1:1) to afford a crude extract which was fractionated on a silica gel column to afford four fractions. The first fraction which showed antimycobacterial activity was further chromatographed in a silica gel column to afford bioactive fractions from which four compounds were obtained. The crude extract, fractions and isolated compounds were screened for their inhibitory effect against Mycobacterium tuberculosis H37Rv (ATCC) using the colorimetric Microplate Alamar Blue Assay (MABA) method. Results: The fractions obtained after silica gel chromatography of the crude DCMmethanol (1:1) revealed the first fraction (MIC=64 mg/mL) to exert the highest inhibition against M. tuberculosis H37Rv. Further separation of this fraction afforded sub-fractions with moderately strong inhibitory activity against the test organism (MIC up to 64 mg/mL). Chromatographic purification of sub-fraction 1 afforded four compounds which were spectroscopically identified as \β-amyrin 3-palmitate (1), squalene (2) and a 1:1 mixture of the sterols \β-sitosterol (3) and stigmasterol (4). Evaluation of the antimycobacterial activity of 1\–4 showed insignificant inhibitory activity against the test organism (MIC = \>128 mg/mL). Conclusion: The present results demonstrate the potential of Abutilon indicum as a plant source of compounds that may exhibit promising antituberculosis activity. While the known compounds isolated for this plant did not show antimycobacterial activity, the obtained results are considered sufficient reason for further study to isolate the metabolites from A. indicum responsible for the antitubercular activity.

Key words:Abutilon indicum, Malvaceae, antitubercular, squalene, \β-amyrin 3-palmitate, \β-sitosterol, stigmasterol.

}, keywords = {Abutilon indicum, Antitubercular, Malvaceae, Squalene, Stigmasterol., β-amyrin 3-palmitate, β-sitosterol}, author = {Allan Patrick G. Macabeo and Christopher A. Lee} }