@article {1506, title = {Antihyperglycemic Activity and Standardization of the Bioactive Extract of Cleome droserifolia Growing in Egypt}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {2nd July 2014}, pages = {15-21}, type = {Original Article}, abstract = {

Background:Cleome droserifolia herb is well known in the Egyptian folk medicine for the treatment of diabetes. However, a standardized active extract of the herb was never prepared for incorporation into a pharmaceutical dosage form. Materials and Methods: Comparative high performance liquid chromatography (HPLC) chromatographic profi les were established in order to study the ethnopharmacological use of the antihyperglycemic herb using a validated reversed phase- HPLC method which was developed for standardization of the active aqueous extract. A biologically guided fractionation of the antihyperglycemic aqueous extract was carried out in vivo using alloxan induced diabetic rats. Results: The aqueous extract contained the highest percent of the total active fl avonol glycosides (78.20\%) compared to the 70\% and 50\% ethanolic extracts (51.17 and 42.66\%, respectively). The aqueous extract and its ethyl acetate fraction possessed the highest antihyperglycemic activities. A standard calibration curve, established for the major bioactive methoxylated fl avonol glycoside (kaempferol-4\’-methoxy-3,7-dirhamnoside) at a concentration range of 44-174 \μg/ml, showed good linearity with a correlation coeffi cient (R2) of 0.998. The recovery of the method was 100.5\%. A high degree of precision (relative standard deviation values \<5\%) was achieved. The limits of detection and quantifi cation were 0.01 and 0.02 \μg/ml, respectively, indicating the sensitivity of the method. Conclusion: The aqueous extract contained the highest percent of the total active fl avonol glycosides. The extract, standardized to contain not \<1.5 \± 0.06\% of kaempferol-4\’-methoxy- 3,7-dirhamnoside, was tested at three different dose levels showing a 63.3\% activity of that of metformin at100 mg/kg body weight. Furthermore, it raised the blood insulin level by 146.26\% at this dose level.

Key words: Antihyperglycemic, Cleome droserifolia, high performance liquid chromatography standardization, kaempferol-4\’-methoxy-3,7-dirhamnoside, validation.

}, keywords = {7-dirhamnoside, Antihyperglycemic, Cleome droserifolia, high performance liquid chromatography standardization, kaempferol-4{\textquoteright}-methoxy-3, Validation}, author = {Amira Abdel Motaal and Shahira M. Ezzat and Hesham El-Askary} }