@article {254, title = {Chemical Profile and Antioxidant Properties of Mundulea sericea}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {213-220}, type = {Original Article}, chapter = {213}, abstract = {

Objectives: To evaluate the phytochemical composition and the antioxidant activity of aqueous-methanolic (20:80) leaves extract of Mundulea sericea Willd. Methods: The extract of leaves was tested for antioxidant activity using various in vitro models viz., 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide, ABTS, ferric reducing antioxidant power (FRAP), total antioxidant activity and reducing power. The phytochemical composition (GC-MS and HPLC) along with total phenolic and flavonoid content of the extract at different concentrations were also determined. Results: Total phenolic and flavonoid contents were found to be equivalents to 107.86 \± 0.53 g of gallic acid and 44.53 \± 0.156 g of rutin /mg of dried hydro-methanolic methanolic extract, respectively. Among various antioxidant assays performed, maximum inhibition was observed for ABTS (IC50 13.26 \± 0.396 g) followed by DPPH (IC50 79.83 \± 0.306 g) and NO (IC50 6.35 \± 0.23 g/mL) assay. The GC-MS analysis revealed over 38 compounds; the prevailing compounds were Sec- Butyl ethyl sulfoxide and Di-methyl sulfoxonium formyl methylide. The RP-HPLC analysis further confirmed the presence of rutin, cinnamic acid and salicylic acid. Conclusion: The results of the present study demonstrated that Mundulea sericea leaves possess high phenolic with flavonoid contents and also revealed potential antioxidant activity so these leaves could be used as a viable source of natural antioxidants for industrial and pharmaceutical preparations.

}, keywords = {Antioxidant, Flavonoids, GC-MS, Leaf extract, Mundulea sericea, Phenolics, RP-HPLC}, doi = {10.5530/pj.2017.2.36}, url = {http://phcogj.com/fulltext/303}, author = {Mahendra Shivshankar Khyade and Mohan Baban Waman} } @article {268, title = {Determination of Rubraxanthone in the Latex of Asam Kandis (Garcinia cowa Roxb) by Reverse Phase High Performance Liquid Chromatography.}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {288-291}, type = {Original Article}, chapter = {288}, abstract = {

Context: Rubraxanthone is a major compound found in Garcinia cowa Roxb which has various biological activities. This compound is likely to be responsible for the pharmacological activities of this plant. The latex of this plant was one of the source of this compound. To prevent counterfeiting, it is essential to develop a method of analysis to determine the levels of these compounds in the latex of G. cowa. Aims: To develop and validated a reverse phase-high performance liquid chromatography (RP-HPLC) technique for determination of rubraxanthone in the latex of G. cowa. Settings and Design: RP-HPLC analysis. Methods and Material: The sample was powdered and dissolve in methanol and then subjected to Reverse Phase High Performace Liquid Chromatoraphy (RP-HPLC). Separation was carried out in a reversed-phase column Shimadzu Shimp-pack VP\–ODS (4.6 x 250 mm). The elution was performed with isocratic solvent using formic acid 0.4 \% v/v in methanol (15:85) with a flow rate 1 ml/minute. The solvents used for the mobile phase were filtered through membrane filter (0.45 mm pore size) and degassed before use. Total running time was 20 minutes and the sample injection volume of injection was 20 ml. While the wavelength of the UV-VIS detector was set at 243.2 nm. Results: The detection and the quantitation limits of rubraxanthone were 1.119 mg/mL and 3.731 \μg/mL, respectively. A regression analysis was performed, with the observation of good linearity (r = 0.998). The values obtained for precision and accuracy determination are in agreement with ICH guidelines. It was found that rubraxanthone in dichloromethane extract of latex G. cowa was 56.56\%. Conclusions: The results demonstrated that the developed method is a reliable HPLC technique for determination of rubraxanthone in the latex of G. cowa.

}, keywords = {Counterfeiting, Isocratic method, Latex of Garcinia cowa Roxb, RP-HPLC, rubraxanthone, Standarization}, doi = {10.5530/pj.2017.2.50}, url = {http://phcogj.com/fulltext/317}, author = {Dachriyanus and Nova Susanti Asjar and Meri Susanti} } @article {440, title = {Quantitative Analysis of Irigenin in the Different Species of Iris Plant by RP- HPLC and its Efficacy Against Different Plant Pathogens}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {November 2017}, pages = {s23-s27}, type = {Original Article}, chapter = {s23}, abstract = {

Irigenin belongs to family of isoflavonoids, isolated from Iris Plant of Kashmir Himalaya. A rapid and specific reverse phase high pressure chromatography (RP HPLC) method for quantitative analysis of irigenin in the different species of Iris Plant was developed. The samples were analyzed on RP-C18 e column (chromolith, 5\μm, 4.6\×100 mm). The HPLC system was operated at ambient temperature (\±30c). The mobile phase consisted of methanol: water. The detecting wavelength at 260 nm and flow rate of 0.6 ml/min. The standard irigenin was diluted using the mobile phase at a known concentration of 1mg/ml; the sample was filtered through sample filter of 0.45 \μ pore size. The filtrate was introduced on to a reverse phase analytical column. The content of irigenin in the different species of Iris Plant was determined. The HPLC showed an excellent performance in separating the irigenin in different species of Iris Plant. Furthermore, the antipathogenic activity. The test compound at each respective concentration was found to be statistically superior against scab. Furthermore, the test compound @ 5000 ppm proved significantly most effective by providing (82.49\%) inhibition in the mycelia growth of apple scab. It was followed by fusarium (77.27\%) at 5000 ppm. Lowest reduction in mycelia growth (65.78\%) was recorded in marssonina and did not differ significantly from Alternaria (67.47\%) at 5000 ppm. Furthermore, lowest inhibition of mycelia growth was recorded at 1000 ppm. Similar trend was recorded for rest of the pathogens i.e. highest reduction at 5000 ppm, lowest at 1000 ppm and at 2000, 3000 and 4000 ppm it ranges between the first two but increases with increase in concentration. From this study irigenin is potent compound which can be used for controlling the growth of respective pathogens.

}, keywords = {Bio efficacy, Irigenin, Iris Plant, RP-HPLC}, doi = {10.5530/pj.2017.6s.153}, url = {http://fulltxt.org/article/377}, author = {Sajad Hassan Wani and Hilal Ahmad Bhat and Javid Iqbal Mir and Shahid Ali Akbar and Sajad Un Nabi and Desh Beer Singh and Nazeer Ahmad} } @article {128, title = {Validated analytical RP-HPLC method for quantitation of wedelolactone from Eclipta alba and marketed Ayurvedic formulations}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {December 2015}, pages = {132-139}, type = {Original Article}, chapter = {132}, abstract = {

Introduction: Eclipta alba Linn. (Asteraceae) is an important ingredient of several Ayurvedic formulations. The monograph on different parts of plant like flowers, leaves, roots are listed in Ayurvedic pharmacopeia of India. The plant is reported to be effective for broad range maladies like inflammation, reproductive problems of females etc. It is also used as a hepatoprotectant, analgesic, antibacterial and antidiabetic agent. Wedelolactone is used as a bioactive marker to establish the quality of the crude drug and its formulations. In the present study, wedelolactone-based standardization of Eclipta alba and its quantitation from marketed herbal and Ayurvedic formulations has been documented using RP-HPLC. Methods: In the current work, an isocratic method has been developed and validated to quantitate wedelolactone from whole plant of Eclipta alba. This method is validated as per ICH guidelines and is used to quantitate the content of wedelolactone in polyherbal formulations like Liv52, Geriforte, Mahabhringaraj oil etc. Results: The LOD is found to be 0.5 \μg/mL and the LOQ is 1 \μg/mL. The linearity range of response is from 5 \μg/mL to 100 \μg/mL. Conclusion: The validated method is found to be simple, sensitive, accurate, rugged and reproducible. This developed method can be recommended for quality assurance and marker-based standardization of polyherbal formulations containing whole plant of Eclipta alba.

}, keywords = {Eclipta alba, Formulations, RP-HPLC, Validation, Wedelolactone., Whole plant}, doi = {10.5530/pj.2016.2.6}, author = {Sunita Shailajan and Sasikumar Menon and Dipti Singh and Gauri Swar} } @article {1489, title = {Development and validation of a RP-HPLC method for the simultaneous determination of Mangiferin, Ellagic acid and Hydroxycitric acid in polyherbal formulation}, journal = {Pharmacognosy journal}, volume = {6}, year = {2014}, month = {8th April 2014}, pages = {23-28}, type = {Original Article}, abstract = {

The US patented polyherbal formulation for the prevention and management of Type II diabetes and its vascular complications was used for the present study. The formulation consists of roots of Salacia species, leaves of Lagestroemia parviflora and fruit rind of Garcinia indica. The use of reversed phase C18 HPLC column was used and eluted with isocratic mobile phase of acetonitrile and phosphoric acid buffer solution enabled the efficient separation of chemical markers within 20min. Validation of the method was performed in order to demonstrate its selectivity, accuracy, precision, repeatability and recovery. All calibration curve shows good linear correlation coefficients (r2\>0.995) within tested ranges. Three markers in this polyherbal formulation were quantified were Mangiferin (1.53\% w/w), Ellagic acid (0.9655 w/w), Hydroxycitric acid (5.3\% w/w). Intra and inter day RSDs of retention times and peak areas were less than 3\%. The recoveries were between 95\% and 102.5\%. In conclusion a method has been developed for the simultaneous quantification of three markers in this polyherbal formulation. The established RP-HPLC method was simple, precise and accurate and can be used for the quality control of the raw materials as well as formulations.

Key words: Polyherbal formulation, Mangiferin, Ellagic acid, Hydroxycitric acid, RP-HPLC.

}, keywords = {Ellagic acid, Hydroxycitric acid, Mangiferin, Polyherbal formulation, RP-HPLC}, author = {Ananth Kumar Kammalla, and Mohan Kumar Ramasamy, and Agarwal Aruna, and Dubey GP, and Ilango Kaliappan} }