@article {1876, title = {Antiurolithiatic Activity of Aqueous Extract of Ziziphus lotus on Ethylene Glycol-Induced Lithiasis in Rats }, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {October 2022}, pages = {596-602}, type = {Research Article}, chapter = {596}, abstract = {

In Morocco, Ziziphus lotus is commonly used as an urolithiatic agent in the traditional medicine. To confirm this effect, an aqueous extract of Ziziphus lotus (AEZL) has been studied in ethylene glycolinduced urolithiasis model of rats. 30 male rats were randomly divided into five groups of six animals each. Group I served as a vehicle control and received distilled water (0.5 ml/100 g p.o.). All remaining groups received calculi inducing treatment for 28 days, comprised of 0.75\% v/v ethylene glycol with 1\% w/v ammonium chloride in drinking water ad libitum for 3 days followed by only 0.75\% v/v ethylene glycol for 25 days. Group II served as lithiatic control and received distilled water (0.5 ml/100 g p.o.). Group III served as curative treatment group and received AEZL at doses of 150 mg/kg from 14th day to 28th day. Group VI served as preventive treatment group and received AEZL at doses of 150 mg/kg from 1st day to 28th day. Group V served as therapeutic and received a drug {\textquotedblleft}Cystone{\textquotedblright} at dose of 750 mg/kg from 14th day to 28th day. The extract treatment decreased the levels of oxalate and calcium in urine. Crystalluria analysis showed that untreated rats excreted large CaOx monohydrate and few dihydrate crystals while treated animals excreted mostly small CaOx dihydrate crystals. Significant similarity was observed between preventive and therapeutic anti-urolithiatic effect of AEZL and anti-urolithiatic effect of cystone (P\<0.001). These results demonstrated that AEZL have an anti-urolithiatic effect with preventive and therapeutic treatments in this experimental condition.

}, keywords = {Ethylene glycol, Rats, Urinary parameter, Urolithiasis, Ziziphus lotus}, doi = {10.5530/pj.2022.14.141}, author = {Miloud Chakit and Rezklah Boussekkour and Aboubaker El Hessni and Youssef Bahbiti and Redouan Nakache and Hicham El Mustaphi and Abdelhalim Mesfioui} } @article {1532, title = {Study on the antioxidant activities of root extracts of Zizyphus lotus from the western region of Algeria}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {26th May 2014}, pages = {32-42}, type = {Research Article}, abstract = {

Objective: To find a new natural source of antioxidant, the phenolic contents and antioxidant activities of some secondary metabolites extracted from roots of Ziziphus lotus were evaluated. Methods: The total polyphenol contents were determined spectrophotometrically. Pyrrocatechol, catechin and cyanidin equivalents were used for these parameters. The antioxidant activities of the extracts were determined by several in vitro systems of assays, namely DPPH radical scavenging activity method, b-Carotene Bleaching assay (BCB), Ferric Reducing Antioxidant Power assay (FRA P) and Total Antioxidant capacity test (TAC). Results: The quantitative estimation showed that the roots of Z. lotus were rich in polyphenols ( 20.09 mg PE/g DW) and proanthocyannidins (1.56\%) and they contained a small amount of flavonoids (0.02 mg CE/g DW). The most fractions exhibited high antioxidant activities, and some even showed higher potency than the standard synthetic antioxidants in some instances. In DPPH assay, all extracts had shown significant inhibition (58.535 \– 94.730\% at 1 mg/mL). In addition, the IC50 values ranged from 0.211 to 0.816 mg/mL, compared to 0.110, 0.214, 0.214 and 0.413 mg/mL for gallic acid, tannic acid, butylated-hydroxyanisol and ascorbic acid respectively. In (BCB) assay, the extracts showed strong inhibition (55.55 \– 100.00\% at 1 mg/mL) and the IC50 values ranging from 0.123 to 0.850 mg/mL compared to 0.433 mg/mL for gallic acid. A dose dependant curve was obtained for all extracts in the FRAP assay. However, the antioxidant potencies of ascorbic acid and extracts were comparable at low concentrations. The majority of extracts showed the highest value of antioxidant activity, based on TAC test (0.073 \– 0.398 mg ascorbic acid / mg extract). Conclusion: The results indicate that Z. lotus roots could be an important sources of natural antioxidants.

Key words:Ziziphus lotus, Polyphenol contents, DPPH radical scavenging activity assay, b-Carotene Bleaching assay, Ferric Reducing Antioxidant Power assay, Total Antioxidant Capacity test.

}, keywords = {b-Carotene Bleaching assay, DPPH radical scavenging activity assay, Ferric Reducing Antioxidant Power assay, Polyphenol contents, Total Antioxidant Capacity test, Ziziphus lotus}, author = {Meriem GHALEM and Salima MERGHACHE and Meriem BELARBI} }