@article {1994, title = {The Effect of Sapodilla Leaf Extract (Manilkara zapota L.) on Lipid Profiles of Alloxan-Induced Diabetic Mice}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {April 2023}, pages = {286-289}, type = {Original Article }, chapter = {286}, abstract = {

The aim of this study is to examine the effect of sapodilla leaf extract on lipid profiles of alloxan-induced diabetic mice. This research method are 30 male mice were used as experimental animals, which were randomly divided into five groups, each group consisting of 6 mice. The division of the group is as follows: Treatment of non-diabetic mice, diabetic mice, diabetic mice by administering pioglitazone at a dose of 2 mg/kg BW, extracts of manila sapodilla leaf (M. zapota L.) dose 100 mg/kg BW and 300 mg/kg BW. Observations were made on the 14th day, after administration of sapodilla leaf extract, mice were given light anesthesia and serum lipid profiles Total Cholesterol (TC), triglycerides (TG), High-Density Lipoprotein (HDL), and Low-Density Lipoprotein (LDL) were measured using diagnostic kits (Pars Azmoon Kit) and automatic analyzer (Abbot, model Alcyon 300). The results of this study are in TC, there was a significant difference in pioglitazone and 100 mg leaf extract against all treatment groups. In TG, there was a significant difference in pioglitazone and 300 mg leaf extract against all treatment groups. In LDL, there were significant differences in the 100 mg, 300 mg leaf extract and pioglitazone, against negative control, and diabetes control. In HDL, there was a significant difference in negative control and 300 mg leaf extract, against 100 mg leaf extract, pioglitazone, and diabetes control. The conclusions of this study exhibited the ethanol extract of M. zapota leaves contains several phytochemical compounds including alkaloids, flavonoids, saponins, polyphenols, tannins, quinones, and steroids. M. zapota leaves extract (100 mg/kg and 300 mg/kg) exhibited a significant effect on improvement in lipid protein.

}, keywords = {Alloxan, Diabetes, Lipid Protein, Manilkara zapota}, doi = {10.5530/pj.2023.15.40}, author = {Tridiganita Intan Solikhah and Tania Anggreani Wijaya and Salsabila and Daffa Alice Pavita and Ragil Kusnandar Miftakhurrozaq and Hartanto Mulyo Raharjo and Maya Nurwartanti Yunita and Faisal Fikri} } @article {1682, title = {Effect of Muntingia calabura L. Leaf Extract on Blood Glucose Levels and Body Weight of Alloxan-Induced Diabetic Mice}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1450-1455}, type = {Research Article}, chapter = {1450}, abstract = {

Objective: To evaluate the effect of Muntingia calabura L. leaf extract on the blood glucose level and weight of alloxan-induced diabetic mice. Methods: The mice were injected using 150mg/kg of alloxan intraperitoneally to induce diabetes. Blood glucose level was tested before alloxan injection and 5 days after injection to confirm diabetes development. M. calabura leaf extract with 100 and 300 mg/kg and 600 μg/kg of glibenclamide was given orally for 14 days. Results: The statistical results showed a significant decrease in blood glucose level, especially on day-7 and day-14 in the M. calabura leaf extract treatment group and glibenclamide treatment group compared to the model control group. There was an increase of weight on day-7 and day-14 in the M. calabura leaf extract group and a significant decrease in weight on day-7 in the glibenclamide group compared to the model control group. Conclusion: M. calabura leaf extract had a significant antidiabetic effect that can normalize the weight of alloxan-induced diabetic mice.

}, keywords = {Alloxan, Diabetes, Mice, Muntingia calabura leaf}, doi = {10.5530/pj.2021.13.184}, author = {Tridiganita Intan Solikhah and Gahastanira Permata Solikhah} } @article {1686, title = {Effect of Muntingia calabura L. Leaf Extract on Blood Glucose Levels and Body Weight of Alloxan-Induced Diabetic Mice}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1450-1455}, type = {Research Article}, chapter = {1450}, abstract = {

Objective: To evaluate the effect of Muntingia calabura L. leaf extract on the blood glucose level and weight of alloxan-induced diabetic mice. Methods: The mice were injected using 150mg/kg of alloxan intraperitoneally to induce diabetes. Blood glucose level was tested before alloxan injection and 5 days after injection to confirm diabetes development. M. calabura leaf extract with 100 and 300 mg/kg and 600 μg/kg of glibenclamide was given orally for 14 days. Results: The statistical results showed a significant decrease in blood glucose level, especially on day-7 and day-14 in the M. calabura leaf extract treatment group and glibenclamide treatment group compared to the model control group. There was an increase of weight on day-7 and day-14 in the M. calabura leaf extract group and a significant decrease in weight on day-7 in the glibenclamide group compared to the model control group. Conclusion: M. calabura leaf extract had a significant antidiabetic effect that can normalize the weight of alloxan-induced diabetic mice.

}, keywords = {Alloxan, Diabetes, Mice, Muntingia calabura leaf}, doi = {10.5530/pj.2021.13.184}, author = {Tridiganita Intan Solikhah and Gahastanira Permata Solikhah} } @article {1009, title = {Comparative Study of the Effects of Annona muricata and Tapinanthus globiferus Extracts on Biochemical Indices of Diabetic Rats}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2018}, pages = {1365-1370}, type = {Research Article}, chapter = {1365}, abstract = {

Introduction: Annona muricata and Tapinanthus globiferus (mistletoe) are medicinal plants used in traditional medicine to combat an array of human diseases. However, there is dearth of scientific prove for the activity of these plants in the management of non-infectious human diseases such as cancer, insomnia, hypertension, ulcers and diabetes. Method: The present study was designed to assess comparatively the effects of methanol extracts of Annona muricata (AME) and Tapinanthus globiferus (TGE) on biochemical indices of alloxan induced diabetic rats. The animals were allocated to eight (8) groups, group I were normal non-diabetic rats, group II (diabetic untreated group), group III treated with metformin alone, groups IV \& V were diabetic rats treated with AME at dose of concentration 200 mg/kg BW \& 400 mg/kg BW respectively, groups VI \& VII were diabetic rats treated with 200 mg/kg BW \& 400 mg/kg BW of TGE respectively. Group VIII were diabetic rats treated with AME + TGE at concentration of 200 mg/kg BW each. Concentrations of serum total cholesterol (TC), high density lipoprotein (HDL), triglyceride (TG) of alloxan diabetic rats, creatinine and urea were assayed after 21 days of the respective treatments. Results: Diabetic untreated rats had significant increase p\<0.05 in TC, TG, urea and creatinine compared to normal control group as opposed to the significant decrease observed for HDL in diabetic rats. However, treatment of diabetic rats with AME and TGE significantly suppressed (p\<0.05) the concentrations of TC, TG and creatinine when compared with the diabetic untreated rats. A similar trend was observed for the calculated atherogenic index of treated rats when compared with diabetic untreated rats. Interestingly, co-administration of AME + TGE improved the biochemical indices of diabetic rats better than using each plant separately. Conclusion: The results of this study suggest that AME and TGE could offer some benefits in the management of diabetic complications especially with regard to cardiac and renal health.

}, keywords = {Alloxan, Annona muricata, Diabetes, Mistletoe, Tapinanthus globiferus}, doi = {10.5530/pj.2019.11.211}, author = {Atanu FO and Avwioroko OJ and Ilesanmi OB and Oguche M} } @article {932, title = {Effect of Diallyl Disulphide (DADS) on Gluconeogenesis: A Study in Isolated Alloxan Induced Diabetic Liver}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {July 2019}, pages = {777-781}, type = {Original Article}, chapter = {777}, abstract = {

Background: Coordinated regulation between cellular glucose uptake and endogenous glucose production is indispensable for maintenance of constant blood glucose concentrations. Liver contributes significantly by altering levels of hepatic glucose release, through controlling gluconeogenesis. Objective: To assess effect of Diallyldisulphide on hepatic gluconeogenesis by assessing gluconeogenic substrates and activity of regulatory enzyme glucose-6-phosphatase and to compare the Diallyldisulphide effect on gluconeogenesis with Metformin. Methodology: Wistar albino rats were categorized into four groups; group Inormal control. Group II, III and IV rats were administered single intraperitoneal injection of alloxan monohydrate 150 mg/kg body weight. Rats were maintained on rat diet and water adlibitum for 30 days. On 31st day rats were sacrificed and procured liver tissues. Each liver tissue cut into small slices and group-III and group-IV liver tissues were separately incubated with Diallyldisulphide 4 mg/g liver tissues and Metformin 4 mg/g liver tissues for 60 minutes at 37oC. The total amino acids and total keto acids levels were estimated after exposure. And post exposure effect on activity of glucose -6-phoshatase and transaminases were also estimated. Results: Alloxan induced diabetes resulted significant raise in total amino acids, total ketoacids, glucose -6-phosphatase and transaminases activities. Whereas incubation of liver tissues with diallyldisulphide significantly reduced total amino acids, total ketoacids, glucose-6-phosphatase, transaminases activities and ratio of total amino acids and ketoacids as compared to diabetic control rat liver tissues. Conclusion: Study suggested that diallyldisulphide suppressed gluconeogenesis as liver glucose-6-phosphatase activity is significantly decreased and precursors for gluconeogenesis were significantly reduced. Suggesting Diallyldisulphide has role in gluconeogenesis and can hold potentials for glucose homeostasis.

}, keywords = {Alloxan, Diabetes mellitus, Diallyldisulphide, Gluconeogenesis, Glucose-6-phosphatase, Total amino acid, Total keto acid}, doi = {10.5530/pj.2019.11.123}, author = {Prashanth kumar Goudappala and Ethirajan Sukumar and C V Yogaraje-Gowda and Kashinath RT} } @article {409, title = {To Evaluate the Antidiabetic and Rejuvenating Capability of Tissues on Alloxan Induced Diabetic Rats under the Effect of Ethanolic Leaf Extract of Coriandrum sativum: A Histopathological Study}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {September 2017}, pages = {792-798}, type = {Original Article}, chapter = {792}, abstract = {

Objective: To evaluate the antidiabetic and rejuvenating capability of tissues on alloxan induced diabetic rats under the effect of ethanolic leaf extract of Coriandrum sativum. Methods: Diabetic model was prepared by administration of alloxan monohydrate (150 mg/ kg i.p). The ethanolic leaf extracts of Coriandrum sativum at a dose of 200 and 400 mg/kg of body weight were administrated to diabetic induced groups for a period of 28 days. The effect of ethanolic leaf extract of Coriandrum sativum leaf extract on serum blood glucose, insulin, lipase, \α- amylase and LDH as well as kidney function test [urea, uric acid, albumin, protein and creatinine] were measured in the alloxan induced diabetic rats. Results: In the acute toxicity study, ethanolic leaf extract of Coriandrum sativum leaf was non-toxic at 2 000 mg/kg in rats. The increased insulin level, albumin and protein level, decreased blood glucose and other biochemical parameters level were observed in diabetic rats treated with both doses of ethanol extract of Coriandrum sativum leaf compared to diabetic control rats. In Histopathological study were revealed toward normal. Conclusion: Ethanolic extract of Coriandrum sativum leaf possesses significant antidiabetic and rejuvenating capability of tissues.

}, keywords = {Alloxan, Coriandrum sativum., Pancreas and Kidney Tissue, Wistar Rats}, doi = {10.5530/pj.2017.6.124}, url = {http://fulltxt.org/article/177}, author = {Bhaskar Sharma and Gaurav Sharma and Suresh Chand Joshi and Sunil Kumar Singh} } @article {143, title = {Evaluation of Antihyperglycemic and Antihyperlipidemic Activity of Leaf Extracts of Breynia vitis-idaea in Alloxan Induced Diabetic Rats}, journal = {Pharmacognosy Journals}, volume = {8}, year = {2016}, month = {January 2016}, pages = {259-263}, type = {Original Article}, chapter = {259}, abstract = {

Aim: The present study was carried out to evaluate the hypoglycemic and hypolipidemic activity of alcohol and aqueous leaf extracts of Breynia vitis-idaea (Burm. F.) Fischer in alloxan induced diabetic rats. Methods: Diabetes was induced into albino Wistar rats by intraperitonial administration of alloxan (120 mg/kg). Normal and diabetic rats divided into different groups of six each. Alcohol and aqueous leaf extracts (300 mg/kg and 600 mg/kg) and standard drug (Glibenclamide 500 \μg/kg) was administered orally for 21 days. Blood samples were collected from overnight fasted normal and diabetic rats on 0th, 7th, 14th and 21st days of treatment. Hypoglycemic activity was evaluated by measuring serum glucose level and glycosylated haemoglobin level after dosing with aqueous and alcohol extracts. Hypolipidemic activity was evaluated by measuring various biochemical parameters like total cholesterol, triglycerides, low density lipoprotein, very low density lipoprotein, high density lipoprotein and phospholipids. Results: Both the extracts significantly (P\<0.001, p\<0.01) reduced fasting blood glucose of alloxan diabetic rats in a dose-related manner, when compared to control and standard. They also have a significant recovery in the levels of parameters measured in lipid profile, when compared to control and standard group. Conclusion: The present investigation established pharmacological evidence to support the folklore claim that it is used as hypoglycemic and hypolipidemic agent.

}, keywords = {Alloxan, Breynia vitis-idaea, Glibenclamide, Hypoglycemic, Hypolipidemic.}, doi = {10.5530/pj.2016.3.15}, author = {Jagdish Chandra Nagar and Lalit Singh Chauhan} } @article {1542, title = {Evaluation of Anti-Diabetic Activity of Methanolic Extract from the Leaves of Rotula Aquatica Lour in Alloxan-Induced Diabetic Rats}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {18th Feb,2014}, pages = {110-114}, type = {Original Article}, chapter = {110}, abstract = {

Objective: The objective of the present study was to evaluate the anti-diabetic activity of methanolic extract from the leaves of Rotula aquatica lour in Alloxan-induced diabetic rats. Materials and Methods: Diabetes was induced in rat by injection of Alloxan (120 mg/kg, i.p.). Diabetic rats were divided into different groups and methanolic leaves extract of Rotula aquatica lour (RA-ME) was administered at dose ranges of 100\–400 mg/kg, p.o for 21 days. Control group received normal saline (0.9\%) for 21 days. Glibenclamide (5 mg/kg, p.o) was used as standard drug. Blood samples were collected from all the groups and analyzed for serum glucose and lipid levels such as total cholesterol (TC), triglyceride (TG), proteins (TP). RA-ME was also tested for oral glucose tolerance test (OGTT) in normal fasted rats. Results: RA-ME (400 mg/kg, p.o) showed a significant (P\<0.01) reduction of serum glucose level in Alloxan-induced diabetic mice as compared with diabetic control. RA-ME (200 and 400 mg/kg) also showed a significant reduction in serum TC, TG, and TP levels in Alloxan-induced diabetic rats. RA-ME (200 and 400 mg/kg, p.o) significantly (P\<0.01) increased the glucose tolerance in OGTT. Conclusion: The results obtained from the present study revealed the potential anti-diabetic activity of methanolic extract from the leaves of Rotula aquatica lour.

Key words: Alloxan, Anti-diabetic, Glibenclamide, Rotula aquatica lour.

}, keywords = {Alloxan, Anti-diabetic, Glibenclamide, Rotula aquatica lour.}, author = {Bhanu Priya, and Manoj Gahlot, and Punam Joshi, and Sarika Zade, and Ujwala Bagmar} }