@article {2210, title = {Analysis of Hepcidin and Interleukin-6 Levels among Transfusion-Dependent Thalassemia Patients With and Without Alloimmunization/Autoimmunization}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {February 2024}, pages = {60-66}, type = {Original Article}, chapter = {60}, abstract = {

Background: Transfusion-dependent thalassemia (TDT) necessitates regular transfusions, resulting in complications such as iron overload, hemolytic anemia, and the emergence of alloantibodies/ autoantibodies. This situation poses challenges in obtaining compatible transfusions. Excessive iron and chronic hemolysis impact the elevation of Interleukin-6 (IL-6), initiating an inflammatory process that triggers hepcidin formation and influences antibody development. This study aims to analyze disparities in IL-6 and hepcidin levels and establish the correlation between IL-6 and hepcidin in TDT patients with and without alloimmunization/autoimmunization. Methods: Forty whole blood samples were collected from TDT patients with and without alloimmunization/autoimmunization, centrifuged, and the serum extracted, then stored in a refrigerator at -80{\textdegree}C. IL-6 and hepcidin levels were assessed using the ELISA method. The Mann-Whitney U test was employed to evaluate differences in hepcidin and IL-6 levels between the two groups. In contrast, the Spearman Correlation test was utilized to analyze the correlation between hepcidin and IL-6 levels. Results: IL-6 levels in the TDT group with alloimmunization/autoimmunization (3.64 pg/ mL) were significantly higher compared to the TDT group without alloimmunization/autoimmunization (1.41 pg/mL; p \< 0.05). Hepcidin levels in the TDT group with alloimmunization/autoimmunization (2,950.6 pg/mL) were significantly higher compared to the TDT group without alloimmunization/autoimmunization (1,599.6 pg/mL; p \< 0.05). The Spearman correlation test revealed a significant positive correlation between hepcidin and IL-6 levels in TDT patients with alloimmunization/autoimmunization (r = 0.764; p = 0.000). Additionally, a significant positive correlation was observed between hepcidin and IL-6 levels in TDT patients without alloimmunization/autoimmunization (r = 0.559; p = 0.010). Conclusion: IL-6 and hepcidin levels were elevated in TDT patients with alloimmunization/autoimmunization compared to those without. Interleukin-6 and hepcidin exhibited a positive correlation in both transfusion-dependent thalassemia groups.

}, keywords = {Anemia, Antibodies, Hepcidin, Interleukin-6, Transfusion-Dependent Thalassemia}, doi = {10.5530/pj.2024.16.9}, author = {Widya Firly Novitasari and Jusak Nugraha and Mia Ratwita Andarsini and Betty Agustina Tambunan} } @article {1920, title = {Ameliorative Effects of Moringa (Moringa Oleifera Lam.) Leaves Extract on Lead-Induced Oxidative Stress, Hepcidin and δ-Alad Levels in Rat{\textquoteright}s Blood}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {December 2022}, pages = {856-862}, type = {Research Article }, chapter = {856}, abstract = {

Introduction: Lead (Pb) is a toxic heavy metal that cause a lot health problem. Blood, especially hemoglobin and erythrocyte, is the main target of lead poisoning. Literatures explain that moringa has phytochemical contents to reduce heavy metal poisoning. This study aimed to examine ameliorative effects of moringa leaves extract on oxidative stress, hepcidin increasement and δ-alad level decline induced by lead poisoning in the blood of rat model. Methods: This study was completely randomized posttest-control group design. Forty-eight males Rattus norvegicus Wistar strain rat were divided into 4 groups. The control group or G0 (given Pb orally doses of 750 mg/kgBW/day for 7 days and was not given 50\% ethanol extract of moringa leaves/MLEE). Three treatment groups (G1, G2 and G3), all were given Pb at a dose of 750 mg/kgBW/day orally for 7 days, followed by administration of MLEE for 14 days at a dose of 250 mg/kgBW/day, 500 mg/kgBW/day and 1,000 mg/kg/day orally, respectively. Blood samples were taken one day after 14 days of MLEE treatment. Pb levels was examined by AAS and δ-ALAD levels, GSH levels, MDA levels and hepcidin levels examined by ELISA. Results: MLEE doses 1,000 mg/kgBW/day for 14 days increased δ-ALAD levels, GSH levels, hepcidin levels and reduce MDA levels significantly compared to the control group. Conclusion: Moringa leaves ameliorate lead-induced poisoning by reducing oxidative stress, declining hepcidin, and increasing δ-ALAD in the blood of male Rattus norvegicus Wistar strains rats. Moringa leaves is beneficial to address Pb poisoning in the blood through antioxidants, anti-inflammation, and improving δ-ALAD level in the blood of Wistar strain rats.

}, keywords = {Blood, Hepcidin, Lead poisoning, Moringa, Oxidative stress., δ-ALAD}, doi = {10.5530/pj.2022.14.179}, author = {Agung Saprasetya Dwi Laksana and Harianto Notopuro and Arifa Mustika} }