@article {2606, title = {Effects of Hydrolyzed Marine Collagen Peptide Supplementation Combined with Phytonutrients on Skin Biophysical Parameters and Extracellular Matrix Biomarkers in Thai Women: A Prospective Clinical Study}, journal = {Pharmacognosy Journal}, volume = {18}, year = {2026}, month = {June 2026}, pages = {157-163}, type = {Original Article}, chapter = {157}, abstract = {

Background: Skin aging involves progressive deterioration of extracellular matrix components. This study investigated the effects of hydrolyzed marine collagen peptide supplementation combined with phytonutrients on skin biophysical parameters and dermal biomarkers in Thai women. Methods: Sixtythree Thai women aged 40{\textendash}60 years received daily supplementation (10,000 mg marine collagen peptides, Chlorella, grape seed extract, rice ceramides) for 12 weeks. Assessments included Antera 3D imaging, Cutometer elasticity measurements, Tewameter barrier function evaluation, and Corneometer hydration analysis. A subset (n=20) underwent suction blister biopsy for dermal biomarker analysis. Results: Significant improvements were observed in Cutometer parameters: R5 net elasticity (p\<0.001), R6 viscoelasticity (p=0.018), and R7 biological elasticity (p=0.003). Transepidermal water loss (TEWL) decreased by 12.1\% (p=0.007). Antera 3D analysis revealed a significant increase in wrinkle width (p=0.021), indicating wrinkle flattening. Biomarker analysis showed significant increases in fibrillin-1 (+8.34\%, p=0.005) and biglycan (+22.91\%, p=0.036). Glogau photoaging classification improved in 23.8\% of participants. Conclusions: Marine collagen peptide supplementation combined with phytonutrients significantly improved skin elasticity and barrier function, with molecular evidence of extracellular matrix enhancement in Thai women.

}, keywords = {Antera 3D, Barrier function, Cutometer, Fibrillin-1, Marine collagen peptides, Skin elasticity}, doi = {10.5530/pj.2026.18.129}, author = {Uratchat Vichaidit and Pratya Phetkate and Parunkul Tungsukruthai and Kammal Kumar Pawa and Panlop Chakkavittumrong and Chuntida Kamalashiran} } @article {2526, title = {Anti-aging Efficacy and Safety of Topical Application of Two Standardized Fenugreek Seed Extracts on Facial Skin in Women: Randomized, Double-Blind, Placebo-Controlled, Clinical Study}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {August 2025}, pages = {470-479}, type = {Original Article}, chapter = {470}, abstract = {

Introduction: Fenugreek (Trigonella foenum graecum L.) is widely used in culinary and medicinal applications and has recently gained attention for its potential anti-aging properties. Objectives: This randomized, double-blind, placebo-controlled study evaluated the anti-aging efficacy and safety of topical application of flavonoid glycosides-based and eleutheroside-oligosaccharide-based standardized fenugreek seed extract creams, namely INDUS1520 and INDUS1530, respectively. Methods: Seventy-five healthy women aged between 35 and 55 years with mild to moderate facial wrinkles were randomly allocated to receive either INDUS1520, INDUS1530, or a Placebo cream for 60 days. Efficacy outcomes included skin luminescence, color, trans-epidermal water loss, moisture content, gloss, elasticity, firmness, wrinkle depth, surface roughness, and collagen distribution. Safety was assessed using a dermatological assessment and subject-reported intolerance questionnaire for adverse events. Results: INDUS1530 significantly improved skin hydration and wrinkle depth, while INDUS1520 significantly reduced transepidermal water loss compared with Placebo. Both creams showed a trend of decreased skin roughness and increased collagen distribution, although this difference was not statistically significant between the groups. All treatments, including Placebo, increased skin gloss. No adverse events or skin irritation were observed. Conclusions: Both fenugreek seed extract creams were well tolerated and demonstrated potential anti-aging benefits, with INDUS1530 showing more pronounced effects on skin hydration and wrinkle reduction. These findings support the use of standardized fenugreek seed extracts as safe and effective ingredients in anti-aging skin care products.

}, keywords = {Anti-aging, Fenugreek seed extract, Skin elasticity, Skin hydration}, doi = {10.5530/pj.2025.17.60}, author = {Prasad Arvind Thakurdesai and Pallavi Onkar Deshpande and Savita Raju Nimse} } @article {2551, title = {Hydrolyzed Collagen Tonic Drink Prevents Worsening of Skin Parameters and Improves Photoaging Classification in Healthy Thai Women: A Randomized Controlled Trial}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {September 2025}, pages = {644-652}, type = {Original Article}, chapter = {644}, abstract = {

Background: Skin aging represents a significant public health burden globally, with Southeast Asian populations experiencing accelerated photoaging due to intensive environmental ultraviolet exposure. Aim: To evaluate the preventive efficacy of hydrolyzed collagen tonic drink on skin aging parameters in healthy Thai women. Methods: A double-blind, randomized controlled trial was conducted with 135 Thai women aged 40-60 years. Participants were randomized to receive either hydrolyzed fish collagen peptides with supportive nutrients (n=67) or active fruit juice control (n=68) daily for 12 weeks. Primary outcomes included skin microtopography parameters measured by Visioscan, skin elasticity assessed by Cutometer, and clinical photoaging classification using Glogau scale. Secondary outcomes examined molecular biomarker changes in procollagen Type I synthesis. Results: The collagen group demonstrated significantly attenuated skin surface deterioration compared to placebo. The Wrinkles parameter showed significant betweengroup differences at week 8 (-100.43 units, p=0.001). Smoothness parameter exhibited protective effects with significant between-group differences at week 8 (-80.08 units, p=0.002). Gross elasticity improved significantly within the collagen group at week 12 (p=0.033). Procollagen Type I synthesis demonstrated superior maintenance in the collagen group versus placebo (between-group percent change difference, p=0.002). Both groups achieved comparable Glogau classification improvements (23.9\% versus 20.6\%). Conclusion: Daily hydrolyzed collagen supplementation with supportive nutrients effectively prevented skin surface parameter deterioration in Thai women, demonstrating meaningful preservation of skin integrity through medium effect sizes for key parameters. These findings support nutritional approaches as complementary strategies for photoaging management in high ultraviolet-exposure populations

}, keywords = {Dermal aging, Hydrolyzed collagen, photoaging, Skin elasticity, Wrinkle}, doi = {10.5530/pj.2025.17.81}, author = {Uratchat Vichaidit and Chuntida Kamalashiran and Kammal Kumar Pawa and Pratya Phetkate} }