@article {2141, title = {Activity of Moringa Oleifera Lam on Liver Function and Histology in White Male Rats}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {October 2023}, pages = {913-920}, type = {Research Article}, chapter = {913}, abstract = {

Introduction: Moringa oleifera Lam has many pharmacological activities, such as immunostimulants. Aim: This study aims to determine the safety profile of repeated use and routine use of Moringa oleifera Lam on liver function and histology. Material and Method: This study used 36 male white rats as test animals. Moringa leaves were administered orally as standardised ethanol extract at doses of 7, 21, and 140 mg/kg BW for 21 days. The liver function observed was the activity of the ALT enzyme. On days 8, 15, and 22, three animals from each group had their blood collected through the orbital sinuses of the eyes and sacrificed to excise the livers. The parameters observed were ALT enzyme activity, liver ratio value, and histological damage of liver tissue. The two-way ANOVA was used to analyse the data of ALT enzyme activity and organ ratio values, followed by DMRT. In contrast, descriptive analysis was used to describe liver histological damage. Result: Test animals had no toxic symptoms or sudden death during the study. The results showed that ALT enzyme activity was significantly affected by the dose and duration of administration of the ethanol extract of Moringa leaves (p\<0.05). However, on the organ ratio values, there were no significant effects of dose (p\>0.05), but significantly affected by duration of administration (p\<0.05). The liver histological descriptive analysis showed histological differences between the control and treatment groups. The 7 mg/kg BW and 21 mg/kg BW showed liver tissue with average to minimal damage. However, the dose groups at 140 mg/kg BW for 14 and 21 days showed changes in liver histology, although only minimal to mild impairment. Conclusion: It concluded that the administration of extract of Moringa oleifera Lam did not cause severe damage to the liver of white male rats.

}, keywords = {Alanine aminotransferase, Histology., Moringa oleifera L., Subacute toxicity}, doi = {10.5530/pj.2023.15.174}, author = {Yufri Aldi and Aisyah Aisyah and Rahmad Abdillah and Aditya Alqamal Alianta} } @article {177, title = {Acute and sub-acute Toxicity study of Aqueous extracts of Canscora heteroclita (L) Gilg in Rodents}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {June/2016}, pages = {399-410}, type = {Original Article}, chapter = {399}, abstract = {

Background: Canscora heteroclita (C. heteroclita) being used in the Ayurvedic system of medicine in India for treatment of various diseases. No systematic toxicity study for this plant was described. Objective: The present study was undertaken to assess the safety use of this plant in traditional practice. Materials and Methods: The acute oral toxicity study of aqueous extract of Canscora heteroclita (AECH) was carried out as per the OECD guidelines 423 in mice and the sub-acute toxicity was carried out at a dose of 200 mg/kg and 400 mg/kg as per OECD 407 guidelines in male and female rats. Results: Mice administered upto 2000 mg/kg as a single dose orally not caused any signs of toxicity or mortality in mice. In sub-acute toxicity study in rats, AECH at two different daily doses of 200 and 400 mg/kg for 28 days did not cause any significant change including the hematological and biochemical parameters. Histopathological examinations showed normal architecture suggesting no morphological disturbances. Conclusion: No deaths or any signs of toxicity was observed after oral administration in acute toxicity study upto a dose of 2000 mg/kg of AECH in mice and upto a dose of 400 mg/kg of AECH in sub acute toxicity study in rats.

}, keywords = {Acute toxicity, Biochemical, Canscora heteroclita, Histology., Sub-acute toxicity}, doi = {10.5530/pj.2016.4.15}, author = {Rajasekaran Aiyalu and Arivukkarasu Ramasamy} }