@article {1412, title = {Qualitative and Quantitative Analysis of 70\% Ethanol Extract from Ruta angustifolia for Developing Anti-Hepatitis C Agents}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {May 2021}, pages = {682-687}, type = {Original Article}, chapter = {682}, abstract = {

Background: Medicinal plants are potential sources for drug candidates. It possesses with various metabolites which have many pharmacology effects. Ruta angustifolia is one of medicinal plants that has been used traditionally for liver disease. Previous study it has been demonstrated to inhibit hepatitis C virus under in vitro cell culture. It decreased protein NS3 level and gave synergistic effect in combination with simeprevir and telaprevir. This plant provides a prospective candidate to develop as anti-HCV Objective: This study evaluates the phytochemistry screening for qualitative assay and determine the concentration of rutin as marker compound for developing R. angustifolia extract as anti-HCV agent. Materials and Methods: R. angustifolia leaves were extracted with 70\% of ethanol. Extract and rutin were analysis their anti-HCV activity by in vitro culture cells of Huh7it. The concentration of rutin was determine by TLC densitometry. Results: The 70\% ethanol extract of R. angustifolia dan rutin exhibit anti-HCV activities with IC50 value of 2.9 {\textpm} 0.8 μg/ml and 28.1 {\textpm} 5.6 μg/ml, respectively. Screening phytochemistry demonstrated to contain flavonoid, terpenoid, alkaloid and polyphenols. TLC densitometry analysis yield the concentration of rutin in extract 0.06 \%. Conclusion: Extract of 70\% ethanol of R. angustifolia has a potential anti-HCV activity. Extract of R. angustifolia may provide a good candidate for developing anti-HCV agents.

}, keywords = {Hepatitis C Virus, Infectious disease, Medicinal plants, Medicine, Ruta angustifolia, Rutin}, doi = {10.5530/pj.2021.13.87}, author = {Tutik Sri Wahyuni and Adita Ayu Permanasari and Lidya Tumewu and Aty Widyawaruyanti and Achmad Fuad Hafid} } @article {194, title = {Analytical Quantifiation, immunomodulatory and Sulforhodamine B assay studies on Albizia lebbeck Leaves Extracts}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {Oct 2016}, pages = {476-482}, type = {Original Article}, chapter = {476}, abstract = {

Background: Albiza lebbeck leaves have been well known for its ethnopharmacological prospects. Objective: The present study aims three extracts (aqueous, methanolic and hydromethanolic) at two dose levels by oral administration by using immunomodulatory models and in vitro cell lines in correlation to analytical studies. Methods: The extracts were subjected to Haemagglutination Antibody Titre and DTH Delayed-Type Hypersensitivity reaction based on acute toxicity results. Chromatographic studies were undertaken comprising of Fourier Transform Infrared Spectroscopy and High performance Thin layer Chromatography and screened for in-vitro cell lines such as MCF-7 and HCT 15 by Sulforhodamine B Assay Method. Results: No response was shown at 100 mg/kg. Significant immunomodulatory effect was noticed at 200 mg/kg with Haemagglutination Antibody Titre (554.66 \± 102.78, 597.33 \± 85.35, 426.66 \± 53.98) and DTH Delayed- Type Hypersensitivity reaction (0.225\±0.01, 0.21 \± 0.01, 0.23 \± 0.01) which showed decrease in paw volume (after 48 h) in case of Sheep Red Blood Cells, (0.5\×109) used as antigens. Total flavonoids content in the extracts were revealed by methods described by Singleton and Quettier. Flavonols such as rutin and quercetin were detected by Fourier Transform Infrared Spectroscopy based on determination of the functional groups and High Performance Thin layer Chromatography showed well resolved spots. The extracts were screened on in-vitro cell lines (MCF 7 and HCT 15) by using Sulforhodamine B Assay method were unsatisfactory results were obtained at final concentrations of 10 \μg/ml, 20 \μg/ml, 40 \μg/ml, 80 \μg/ml. Conclusion: Thus, present paper suggests that extracts has served as a promising immunomodulator for immune system disorders.

}, keywords = {Delayed-Type Hypersensitivity response, Fourier Transform Infrared Spectroscopy, Haemagglutination Antibody Titre, High performance Thin layer Chromatography, MCF-7., Quercetin, Rutin}, doi = {10.5530/pj.2016.5.11}, author = {Gaurav Mahesh Doshi and Manjushree kundalik Pawar and Kajal Haribhai Chavda} }