@article {1075, title = {Comparative Pharmacognostical and Pharmacological Evaluation of Costus speciosus (Koen) J.E. Sm. Germplasm Collected from Eastern Ghats of India}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {February 2020}, pages = {150-156}, type = {Research Article}, chapter = {150}, abstract = {

Background: Costus speciosus is an erect perennial herb belonging to family Costaceae, an important medicinal plant widely used in several indigenous medicinal formulations. Objective: A comparative evaluation of Pharmacognostical and Pharmacological potential of Costus speciosus for the validation of traditional claims and quality parameters for industry. Materials and Methods: Pharmacognostical studies were performed as per Ayurvedic Pharmacopeia of India and quantification of diosgenin was done through HPTLC. In vitro antidiabetic activity was evaluated by α-amylase inhibition assay based on starch iodine method and in vitro anti-inflammatory were done by using inhibition of protein denaturation assay. Results: The pharmacognostical standards were also laid down for each sample. Morpho-anatomical characters had no distinct variation in all the collected samples of Eastern Ghats. The quantification of diosgenin (without hydrolysis of samples) in the collected germplasm varies significantly from 0.002 to 0.076 \% on dry weight basis. The maximum content was recorded in NBCS-06 from Patiya, Bhubaneswar and was identified as distinct chemotype with high metabolite content. IC50 value of Costus speciosus extract in starch-iodine assay was found to be maximum in NBCS- 6 (87.54 μg/ml) and inhibition of protein denaturation assay was found to be maximum in NBCS- 11 (73.91 μg/ml), respectively. Conclusion: The study suggests that the Costus speciosus germplasm possess potential anti-inflammatory and anti-diabetic activity and comparative pharmacognostical parameters will be useful in collection of location specific potential samples for industrial usage along with quality control of raw materials.

}, keywords = {Anti-diabetic, Anti-inflammatory, Costus speciosus, Diosgenin, HPTLC}, doi = {10.5530/pj.2020.12.22}, author = {Manish Kumar and Ankita Misra and Akanksha Srivastava and Pushpendra Kumar Shukla and L M Tewari and Sharad Srivastava} } @article {195, title = {Evaluation of Costus speciosus in Experimental Models of Depression in Albino Mice}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {Oct 2016}, pages = {483-486}, type = {Original Article}, chapter = {483}, abstract = {

Introduction: Despite theavailability of numerous antidepressant drugs the therapy of depression is far from satisfactory. Two of the many reasons for this are the delay in onset of effect and the adverse effects associated with most of the antidepressants. Costus speciosus rhizome has shown adaptogenic activity. Objective: To evaluate the antidepressant activity of50\% aqueous-ethanol extract of Costus speciosus (CS) leaves in experimental models of depression. Materials and Methods: Male albino mice were randomly assigned to five groups of six each. We studied three doses of the leaf extract (100, 200 and 400 mg/kg) on two models of depression \– forced swim test and tail suspension test. Imipramine was used as the standard control. Both acute and chronic effects were studied. Drugs (test drug, standard control and vehicle) were administeredorally, one hour before the experiment in the acute study and daily for 14 days for the chronic study. Results: Imipramine showed significant antidepressant activity as demonstrated by a reduction in duration of immobility in both acute and chronic studies of forced swim test and tail suspension test. CS showed a dose dependent antidepressant activity. CS_100 demonstrated an antidepressant effect only in the acute forced swim test. CS_200 demonstrated an antidepressant activity in both acute and chronic forced swim test but not in tail suspension test. CS_400 showed maximum antidepressant activity in both acute and chronic studies, which was comparable to that of the standard drug imipramine. Conclusion: CS showed dose dependent antidepressant activity with CS_400 mg/kg showing maximum effect.

}, keywords = {Antidepressant, Costus speciosus, Forced swim test, Tail suspension, Test.}, doi = {10.5530/pj.2016.5.12}, author = {Zahoor Ahmad Rather and Nateshprabhu M and Sushma DS and Rakesh Kb and Sunil Pai and Ullal sheetal D} }