@article {564, title = {Preliminary of Pharmacokinetics Study of Brown Seaweed (Turbinaria decurrens Bory) Extract in Colon Cancer Model Mice Induced by AOM (Azoxymethane) and DSS (Dextran Sodium Sulphate)}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {March 2018}, pages = {567-570.}, type = {Original Article}, chapter = {567}, abstract = {

Introduction: Colon Cancer is one of leading cancer that causes morbidity and mortality worldwide, causes 774,000 deaths. Fucoxanthin contained brown seaweed Bory) extracts showed cytotoxic activity by and study, including colon cancer. This study aims to examine the absorption profile of fucoxanthin in blood plasma on colon cancer model mice. Methods: Mice were induced by azoxymethane (AOM) and two cycle dextran sulfate sodium (DSS). Mice were given an extract of brown seaweed Bory that contain fucoxanthin. Fucoxanthin content in blood plasma analyzed using mobile phase methanol: acetonitrile by Reversed- Phase High-Performance Liquid Chromatography (RP-HPLC) analysis. Results: The maximum concentration of fucoxanthin in blood was at 4 hours after administration, and the plasma concentration is 2.15 nmol/L, T1/2 6.25 h, and AUC 10.75 nmol/L/h Conclusions: The method was applied successfully in a pharmacokinetic study and the resulting oral brown seaweed bioavailability calculated.

}, keywords = {Brown seaweed, Colon cancer, Fucoxanthin}, doi = {10.5530/pj.2018.3.92}, url = {http://fulltxt.org/article/525}, author = {Nurul Qurrota{\textquoteright}Ayun and Astria Deviyani Zakaria and Anton Bahtiar} } @article {219, title = {Cytotoxicity of Fucoidan from Three Tropical Brown Algae Against Breast and Colon Cancer Cell Lines}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {December 2016}, pages = {14-20}, type = {Original Article}, chapter = {14}, abstract = {

Introduction: Fucoidan is a sulfated polysaccharide that has a wide range of bioactivities including anti-cancer. This polysaccharide commonly is extracted from marine brown seaweed. There is lack of information on the fucoidan extracted from tropical brown algae and its anti-cancer activity. Objectives: The objectives of this study were to purify fucoidan from Sargassum sp., Turbinaria sp. and Padina sp., and to evaluate their cytotoxicity against breast cancer (MCF-7) and colon cancer cells (WiDr). Materials and Methods: Fucoidan extraction was conducted by using acid extraction method. Purified fucoidans were obtained by DEAE cellulose column chromatography and confirmed by HPLC and FT-IR spectrometry. The cytotoxicity was evaluated by using the MTT (3-[4,5-dimethylthiazol-2-yl] -2,5- diphenyltetrazolium bromide) assay. Results: Fucoidan from Sargassum sp. and Turbinaria sp. showed low cytotoxicity with IC50 ranging between 461-663 \μg/mL. Higher cytotoxicity against MCF-7 and WiDr was showed by fucoidan from Padina sp. with IC50 of 144 and 118 \μg/mL, respectively. While its IC50 against Vero cells was 501 \μg/mL.Standard fucoidan from Fucus vesiculosus exhibited IC50 of 60, 63 and 211 \μg/mL against MCF-7, WiDr and Vero Cells, respectively. Although the IC50 was higher than that of standard fucoidan, Padina sp. fucoidan showed cytotoxicity comparable with standard fucoidan at concentrations below 100 \μg/mL. Conclusion: These results indicated that Padina sp.fucoidan showed potential selective cytotoxicity, and promising for the development of an anti-cancer compound.

}, keywords = {Breast cancer, Colon cancer, Cytotoxicity., Fucoidan, Phaeophyta}, doi = {10.5530/pj.2017.1.3}, author = {Alim Isnansetyo and Fadilah Nor Laili Lutfia and Muhammad Nursid and Trijoko and Ratna Asmah Susidarti} }