@article {1082, title = {Morpho-anatomical, Phytochemical and TLC/HPTLC Studies on Whole Plant of Plectranthus vettiveroides (Jacob) N.P. Singh and B.D. Sharma}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {February 2020}, pages = {58-65}, type = {Original Article}, chapter = {58}, abstract = {

Background: Plectranthus vettiveroides is an aromatic perennial herb, which belongs to family Lamiaceae. The whole plant of P. vettiveroides is aromatic, bitter, cooling, febrifuge, diaphoretic, stimulant, diuretic, useful in vitiated conditions of pitta, burning sensation, leprosy, skin diseases, leucoderma, fever, vomiting, diarrhoea, ulcers, stomachic and emmenagogue. In the present investigation morpho-anatomical, preliminary phyto-chemical, physico-chemical parameters and TLC/HPTLC fingerprint of the whole plant of P. vettiveroids were carried out and reported. Objective: To carry out morpho-anatomical, phytochemical and TLC/HPTLC studies on dried whole plant of P. vettiveroides for authentication/identification. Methods: The morphology, microscopy and phyto-chemical investigation were carried out by following standard methods. Results: Microscopically spiral, reticulated, scalariform and pitted vessels, libriform and septate fibres, multicellular branched covering trichomes, glandular trichomes and silica crystals are the unique diagnostic characteristics in whole plant of P. vettiveroides. The preliminary phyto-chemical, physico-chemical parameters and number of spots and Rf values of TLC/HPTLC are found to be unique diagnostic characteristics of the plant for identification. Conclusion: The finding of present study is helpful in standardization of formulation consists of whole plant of P. vettiveroides as ingredient in their powdered form and also for authentication/ identification of dried whole plant of P. vettiveroides.

}, keywords = {Morpho-anatomy, P. vettiveroides, Phytochemistry, standardization, TLC/HPTLC finger print}, doi = {10.5530/pj.2020.12.10}, author = {Velvizhi D and Nartunai G and Susikumar S and Ilavarasan R} } @article {224, title = {Micro-morphoanatomical approach for comparative analysis of Tinospora cordifolia (Willd.) Miers and its adulterant plant using SEM and Cryostat}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {December 2016}, pages = {39-45}, type = {Original Article}, chapter = {39}, abstract = {

Background: Tinospora cordifolia (Willd.) Miers, belongs to Menispermaceae, commonly known as \“Guduchi\”or \“Amrita\” and has immense importance in Ayurvedic medicine. Several studies have been carried out on pharmacology, pharmaceutical, anticancer activity and clinical trials of T. cordifolia, however not much information is available on the adulterants that are added with the genuine plant. Considerable work has been done on pharmacognosy but very few studies have been performed on the anatomy of T. cordifolia. Objectives: Comparative micro-morphoanatomical analysis of T. cordifolia and P. daemia, is important for quality control of fresh and dried samples of the root, stem and leaves. Material and Methods: The plant materials of T. cordifolia and P. daemia were collected, and identified. Fixed sample subjected to cryostat microtomy and sections were observed under light microscope. Micromorphology were studied by light as well as scanning electron microscope. Results: Anatomical studies are cheap and important tool for identification of the correct medicinal plant. Adulterant plant does not contain active principles like Berberine and Tinosporin nor have similar morpho-anatomical characters. Because of the similar appearance and resemblance of dried adulterant plant samples, medicinally important T. cordifolia often get adulterated with other plants. SEM study revealed morpho-anatomical differences like the abundance of starch in cortical and ray parenchyma, different types of trichomes, etc. Cryostat techniques were used for sectioning and showed the presence of xylem plates with wide multicelluar rays. Conclusion: Morphological and anatomical differences were observed in T. cordifolia and P. daemia. The given results showed significant differences therefore this study aims to help in accurate identification and avoid adulteration of a medicinally important plant.

}, keywords = {Berberine, Morpho-anatomy, Pergularia daemia, SEM study., Tinospora cordifolia, Tinosporin}, doi = {10.5530/pj.2017.1.8}, author = {Vidya Shivram Patil and Nutan Padmnabh Malpathak} }