@article {441, title = {Distribution, Ethnobotany, Pharmacognosy and Phytoconstituents of Coptis teeta Wall.: A Highly Valued and Threatened Medicinal Plant of Eastern Himalayas}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {November 2017}, pages = {s28-s34}, type = {Original Article}, chapter = {s28}, abstract = {

Objectives:\ To study the distribution, traditional knowledge, dose and preparations, phytoconstituents, pharmacognostic characters and to correlate phytoconstituents and the claimed health benefits among the tribal people of Arunachal Pradesh if any, the present study was carried out. Methods: Field survey and interview were used for ethnobotany and protocol given in Shah and Seth (2010), Kakote et. al.,(2012) and Wallis (2011) was followed to study pharmacognostic studies while GC-MS was used in the studies of phytoconstituents. Results: This study reports numbers of volatile and non-volatile compounds from the rhizome of Coptis teeta with high percentage of berberine alkaloids. The study also reports alkaloid deposition at parenchymatous tissues and vascular tissues of rhizome. Phytoconstituents presents in the ethanolic extract of C.teeta cold be related to health problems and phytoconstituents as claimed by tribal people of Arunachal Pradesh. Conclusion: Locally called Mishmi teeta in Arunachal Pradesh, Coptis teeta Wall. is a well-known medicinal plant used among Mishmi and other tribes of Arunachal Pradesh for health problems like loose motion, stomach pain, diarrhoea and malaria. This endemic and threatened medicinal plant contains numbers of biologically active compounds and need in-situ as well as ex-situ conservation.

}, keywords = {Berberine, Eastern Himalayas, Medicinal plant, Mishmi tribe, Phytoconstituents, Threatened}, doi = {10.5530/pj.2017.6s.154}, url = {http://fulltxt.org/article/378}, author = {Temin Payum} } @article {327, title = {Identification and Characterization of Berberine in Tinospora cordifolia by Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry (LC MS/MS Q-tof) and Evaluation of its anti Inflammatory Potential}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {April 2017 }, pages = {350-355}, type = {Original Article}, chapter = {350}, abstract = {

Introduction: Tinospora cordifolia (Willd.) Miers ex Hook F and Thomas commonly called as gudduchi or amrita is a widely used plant in traditional medicinal system of Ayurveda. A UPLC MS/MS Q-tof method for the identification and characterization of berberine in Tinospora cordifolia (Willd.) Miers. ex HooK.F. and Thomas. and to evaluate the anti inflammatory potential of bioactive fraction. Materials and Methods: The presence of berberine in Tinospora cordifolia was determined by HPLC and was subsequently isolated by HPTLC. The anti inflammatory property of the fraction containing berberine was demonstrated to have an inhibitory activity on 5 lipoxygenase, an enzyme involved in inflammatory pathway and its IC50 value was obtained. The binding interactions between berberine and 5-LOX were demonstrated by docking studies. Result: The presence of berberine in Tinospora cordifolia methanolic extract was identified by HPLC and HPTLC analysis and confirmed by UPLC MS/ MS Q-tof. The fraction containing berberine inhibited 5-LOX with an IC50 of 0.041\± 0.0003\μg/mL as compared to that of NDGA (positive control) which showed an IC50 of 2.75 \± 0.05 \μg/mL. Molecular docking of berberine with 5-LOX showed a binding energy of -8.942 \± 0.039665 kcal/mol and Ki of 273.16 \± 3.026 nM as compared to the NDGA which has a binding energy of -7.186 \± 0.170503 kcal/mol and Ki 5.604\± 1.618 \μM. Conclusion: Tinospora cordifolia can be used as a source of berberine and possible anti inflammatory activity of Tinospora cordifolia may be attributed to the presence of berberine.

}, keywords = {5 Lipoxygenase, Berberine, Mass spectrometry, Molecular Docking., Tinospora cordifolia}, doi = {10.5530/pj.2017.3.59}, url = {/files/PJ-9-3/10.5530pj.2017.3.59}, author = {Mohind C. Mohan and Anu P. Abhimannue and Prakash Kumar B} } @article {224, title = {Micro-morphoanatomical approach for comparative analysis of Tinospora cordifolia (Willd.) Miers and its adulterant plant using SEM and Cryostat}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {December 2016}, pages = {39-45}, type = {Original Article}, chapter = {39}, abstract = {

Background: Tinospora cordifolia (Willd.) Miers, belongs to Menispermaceae, commonly known as \“Guduchi\”or \“Amrita\” and has immense importance in Ayurvedic medicine. Several studies have been carried out on pharmacology, pharmaceutical, anticancer activity and clinical trials of T. cordifolia, however not much information is available on the adulterants that are added with the genuine plant. Considerable work has been done on pharmacognosy but very few studies have been performed on the anatomy of T. cordifolia. Objectives: Comparative micro-morphoanatomical analysis of T. cordifolia and P. daemia, is important for quality control of fresh and dried samples of the root, stem and leaves. Material and Methods: The plant materials of T. cordifolia and P. daemia were collected, and identified. Fixed sample subjected to cryostat microtomy and sections were observed under light microscope. Micromorphology were studied by light as well as scanning electron microscope. Results: Anatomical studies are cheap and important tool for identification of the correct medicinal plant. Adulterant plant does not contain active principles like Berberine and Tinosporin nor have similar morpho-anatomical characters. Because of the similar appearance and resemblance of dried adulterant plant samples, medicinally important T. cordifolia often get adulterated with other plants. SEM study revealed morpho-anatomical differences like the abundance of starch in cortical and ray parenchyma, different types of trichomes, etc. Cryostat techniques were used for sectioning and showed the presence of xylem plates with wide multicelluar rays. Conclusion: Morphological and anatomical differences were observed in T. cordifolia and P. daemia. The given results showed significant differences therefore this study aims to help in accurate identification and avoid adulteration of a medicinally important plant.

}, keywords = {Berberine, Morpho-anatomy, Pergularia daemia, SEM study., Tinospora cordifolia, Tinosporin}, doi = {10.5530/pj.2017.1.8}, author = {Vidya Shivram Patil and Nutan Padmnabh Malpathak} } @article {1526, title = {Physico Chemical Characterization and Anti Inflammatory Activity of Stem Extracts Of Berberis aristata DC and Cosinium fenestratum Linn in Carrageenan Induced Wistar Rats}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {26th May 2014}, pages = {72-77}, type = {Research Article}, abstract = {

An initial study on the physico-chemical properties of Berberis aristata and Cosinium fenestratum, both used as Daruharidra by the Ayurvedic drug industry, recorded all the tested parameters within the limits of Ayurvedic Pharmacopeia of India. Also, analysis of secondary metabolite of the plants was carried out focusing on the presence and quantification of berberine in the samples. Further, the potential difference in the anti-inflammatory activity of the aqueous and methanolic stem extracts of the two species was compared using carrageenan induced Wistar rats model. At a dose of 25 mg/kg, the aqueous and methanolic crude extracts of both the plant species showed significant inhibition of rat paw edema at various time intervals viz.,0, 30, 60, 120 and 240 minutes as against the control standard drug indomethacin (p\<0.01, p\<0.001).

Key words: antiinflammation, Berberine, Berberis aristata, Cosinium fenestratum, Physico-chemical analysis.

}, keywords = {Antiinflammation, Berberine, Berberis aristata, Cosinium fenestratum, Physico-chemical analysis}, author = {S.Tamilselvi and Padma Venkatasubramanian and N.S. Vasanthi} }