@article {2512, title = {Formulation Strategy and Excipient Selection for Tribulus terrestris L. Tablets: A Quality-Based Approach}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {June 2025}, pages = {370-377}, type = {Research Article}, chapter = {370}, abstract = {

Background: To develop and evaluate a tablet formulation containing standardized dry extract of Tribulus terrestris L.{\textemdash}a plant traditionally used in Mongolian medicine{\textemdash}by focusing on excipient optimization and pharmaceutical quality assessment. Methods: Tablets were manufactured using the wet granulation method, incorporating an extract standardized to 54.45\% dioscin. Various excipient ratios were investigated to determine the optimal formulation. Dioscin content was quantified using UV-visible spectrophotometry. Results: The optimized formulation comprised 25\% dry extract, 34\% lactose, 34\% microcrystalline cellulose, 6\% PVPP, 0.5\% magnesium stearate, 0.5\% talc, and 4.5\% HPMC. The tablets demonstrated acceptable pharmaceutical characteristics, including an average weight of 0.113 {\textpm} 0.001 g, hardness of 0.83 {\textpm} 0.23 MPa, friability of 98.17 {\textpm} 0.65\%, and disintegration time of 7.30 minutes. The dioscin content was 13.50 {\textpm} 0.50 mg/g, with an assay recovery of 95.51\%. Dissolution exceeded 83\% in both media within 45 minutes. The similarity factor (f$_{2}$) confirmed equivalent dissolution profiles. Conclusion: The developed T. terrestris tablet formulation met pharmacopoeial standards and exhibited consistent physical, chemical, and biopharmaceutical performance, supporting its potential as a standardized herbal medicinal product for clinical and commercial application.

}, keywords = {Tribulus Terrestre{\textquoteright}s L.; tablet; excipients; saponins; spectrophotometer}, doi = {10.5530/pj.2025.17.46}, author = {Baigalmaa Dovchinsuren and Wang Qin Hu and Jambaninj Dambiinyam and Yerkyebulan Mukhtar and Davaadagva Damdinjav and Enkhjargal Dorjbal} } @article {2261, title = {Factors Affecting the Occurrence of Diarrhea in Infants Aged 7-14 Months in Puskesmas Alak Kupang City}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {April 2024}, pages = {388-392}, type = {Research Article}, chapter = {388}, abstract = {

Diarrhea is a disease characterized by changes in the consistency of stools to liquid accompanied by an increase in the frequency of defecation more than usual \> 3 times / day accompanied by changes, with or without blood and or mucus. Factors that cause diarrheal disease are infections, infections can be caused by bacteria, parasites and viruses. Busy parents often pay less attention to health and the impact of an unhealthy environment. The three children have digestive problems, which are still considered a habit and can be cured without having to go to a health facility. The purpose of this study was to identify and analyze the factors that influence the incidence of diarrheal disease in infants aged 7-12 months at the Alak Health Center, Kupang City. The type of research used is an analytical survey with a case control design. The population in this study was divided into case populations, namely all infants aged 7-12 months totaling 137 infants and controls were infants aged 7-12 months who did not experience diarrhea totaling 8,868 infants. Determination of the size of the control sample using a ratio of 1:1 with the case sample so that the number of control samples is the same as the case sample, namely 37 infants and the total sample is 74 infants. The sampling technique is simple random sampling. Analysis of the data used is the chisquare test. The results showed that there was a significant relationship with a history of premature birth (p-value = 0.015 with an odds ratio = 3.434), low birth weight (p-value = 0.015 with an odds ratio = 4.563), exclusive breastfeeding (p-value = 0.015 with an odds value ratio = 3.434), immunization status (p-value = 0.004 with odds ratio = 4.265) and history of allergies (p-value = 0.004 with odds ratio = 4.083) with the incidence of diarrheal disease at the Alak Health Center, Kupang City. Suggestions that health center health workers need to be able to increase prevention efforts from an early age by approaching the community and providing concrete education related to the incidence of diarrhea.

}, keywords = {BBLR, Diarrhea, Exclusive Breastfeeding and Immunization Status, Premature}, doi = {10.5530/pj.2024.16.60}, author = {Deviarbi Sakke Tira and Sonyalin Buifena and Sigit Purnawan and Maria Magdalena Dwi Wahyuni and Marni Marni and Anwar Mallongi} } @article {2262, title = {Factors Affecting the Realization of Non-Physical Special Allocation Funds in The Health Sector at the Baubau City Health Office}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {April 2024}, pages = {393-399}, type = {Research Article}, chapter = {393}, abstract = {

Failure of meeting the budget realization target can result in the loss of spending benefits, because not all of the funds allocated can be utilized by the government which means there are idle funds. This study aims to investigate the factors that affect budget Realization. This study was approached using mixed method design, using a saturated sample technique where the sample used was the entire population of financial managers within the scope of the Baubau City{\textquoteright}s Health Office and Community Health Center totaling 76 respondents. The data were collected by questionnaire techniques and in-depth interviews with 6 informants and analyzed by univariate analysis techniques, bivariate with Chi Square formula, and multivariate logistic regression analysis. The results showed that budget planning (p = 0.000), budget implementation (p = 0.000), human resources (p = 0.000), regulation (p = 0.001), and leadership (p = 0.012) influence on budget Realization. The results of logistic regression analysis show that budget planning is the most influential factor on budget Realization with p value of 0.002\<0.005 and OR 11.353. Budget planning, budget implementation, human resources, regulations and leadership factors influence budget realization. Related parties are expected to improve the quality of human resources in the field of planning and budgeting division, which in turn, can increase budget realization.

}, keywords = {Budget Realization, Human Resources, Implementation, Leadership, Planning, Regulation}, doi = {10.5530/pj.2024.16.61}, author = {Wildan Harisma and Sukri Palutturi and Amran Razak and Indar and Ummu Salmah and Syamsuar Manyullei and Anwar Mallongi} } @article {2045, title = {Factors Affecting the Long-Term Protection Against Hepatitis B Immunization in Infancy: A Meta-Analysis}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {June 2023}, pages = {385-394}, type = {Research Article}, chapter = {385}, abstract = {

Introduction: Hepatitis B virus (HBV) infection is a major global health issues and one of the most dangerous viral infections with a high mortality rate. Newborns and infant vaccination against chronic HBV infection are crucial for preventing mother-to-child transmission (MTCT). This study aimed to conduct a meta-analysis to investigate the factors affecting long-term protection against Hepatitis B Immunization in infancy. Material and Methods: Our literature searches are from PubMed, Science Direct, Web of Science, and ProQuest publications between January 2000 and December 2021. The included literature assessed the risk of bias using the Newcastle Ottawa Quality Assessment Scale. We identify Hepatitis B surface antibodies (anti-HBs) >= 10 mIU/mL as being protective against HBV infection. The results are combined with a random effect or fixed effect model. Results: Eighteen eligible observational studies with a total of 16,642 participants were included. Analysis of factors affecting long-term protection status by assessing anti-HBs titers showed significant results on several factors, including gestational age for anti-HBs titers (OR 2.5; 95\% CI 1.62-3.85; p\<0.0001), weight for age to anti-HBs titers (OR 1.36; 95\% CI 1.06-1.75; p=0.02), length for age to anti-HBs titers (OR 0.01; 95\% CI 0.01-0.02; p\<0.00001), and immunization status based on the number of vaccine doses (4 doses vs 3 doses) to anti-HBs titers (p\<0.00001). Conclusions: Anti-HBs titers of hepatitis B immunization were significantly affected by gestational age, weight for age, length for age, and vaccine doses. Parents of newborns must be informed about basic immunization and provide adequate nutritional intake to the mother and babies to prevent HBV infection.

}, keywords = {Hepatitis B antibodies, Hepatitis B vaccines, Immunity, Immunization, Infant.}, doi = {10.5530/pj.2023.15.89}, author = {Aldila Pratiwi and Bagus Setyoboedi and Citrawati D. K. Wungu} } @article {2156, title = {Factors Affecting the Occurrence of Diarrhea in Infants Aged 7-12 Months in Puskesmas Alak Kupang City}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {December 2023}, pages = {1024-1028}, type = {Original Article}, chapter = {1024}, abstract = {

Diarrhea is a disease characterized by changes in the consistency of stools to liquid accompanied by an increase in the frequency of defecation more than usual \> 3 times / day accompanied by changes, with or without blood and or mucus. Factors that cause diarrheal disease are infections, infections can be caused by bacteria, parasites and viruses. Busy parents often pay less attention to health and the impact of an unhealthy environment. The three children have digestive problems, which are still considered a habit and can be cured without having to go to a health facility. The purpose of this study was to identify and analyze the factors that influence the incidence of diarrheal disease in infants aged 7-12 months at the Alak Health Center, Kupang City. The type of research used is an analytical survey with a case control design. The population in this study was divided into case populations, namely all infants aged 7-12 months totaling 137 infants and controls were infants aged 7-12 months who did not experience diarrhea totaling 8,868 infants. Determination of the size of the control sample using a ratio of 1:1 with the case sample so that the number of control samples is the same as the case sample, namely 37 infants and the total sample is 74 infants. The sampling technique is simple random sampling. Analysis of the data used is the chisquare test. The results showed that there was a significant relationship with a history of premature birth (p-value = 0.015 with an odds ratio = 3.434), low birth weight (p-value = 0.015 with an odds ratio = 4.563), exclusive breastfeeding (p-value = 0.015 with an odds value ratio = 3.434), immunization status (p-value = 0.004 with odds ratio = 4.265) and history of allergies (p-value = 0.004 with odds ratio = 4.083) with the incidence of diarrheal disease at the Alak Health Center, Kupang City. Suggestions that health center health workers need to be able to increase prevention efforts from an early age by approaching the community and providing concrete education related to the incidence of diarrhea.

}, keywords = {BBLR, Diarrhea, Exclusive breastfeeding, Immunization Status, Premature}, doi = {10.5530/pj.2023.15.188}, author = {Deviarbi Sakke Tira and Sonyalin Buifena and Sigit Purnawan and Maria Magdalena Dwi Wahyuni and Marni Marni and Anwar Mallongi} } @article {1944, title = {Fingerprint and Multivariate Analysis of Apium Graveolens L. From Different Geographic with Spectroscopic ATR-FTIR}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {January 2023}, pages = {1022-1028}, type = {Research Article }, chapter = {1022}, abstract = {

Background: Apium graveolens L. or widely known as celery is a plant that comes from the Apiaceae family. Apium graveolens L. is found in the Indonesian Herbal Pharmacopoeia as a nutritious plant. Plants that spread throughout Indonesia, both highlands and lowlands, contain 3-n-butylphthalide, D-limonene, Linalool, Luteolin, Apigenin and Apiin compounds. Differences in geographical location of growth resulted in differences in the content and concentration of Apium graveolens L. Aim and Objectives: This study was conducted to determine the differences of Apium graveolens L. based on its distribution in Indonesia. Indonesia does not yet have a standard for Apium graveolens L. Material and Methods: this research uses Apium graveolens provided by the Center for Research and Development of Traditional Medicinal and Medicinal Plants (B2P2TOOT) Tawangmangu. 10 samples from different regions in Indonesia will be read using the ATR-FTIR instrument in the finger print area as well as PCA (principal principal) multivariate analysis. component analysis) and HCA (Hierarchical Clustering Analysis). Results: The PCA results show the highest number of variants on PC 1 91,98\% and PC 2 variants at 4,71\%. In the quadrant 4 score plot, it was found that there was a closeness between the standard sample and the S2 sample (Materia Medika Batu). Conclusion: The result of this study is that there is a mutually supportive relationship from spectrum readings, sample location (temperature, soil moisture content, height (m a.s.l) with multivariate analysis (PCA, HCA results) when compared to the geographic location of each sample.

}, keywords = {Apium graveolens L, ATR-FTIR fingerprint area, Multivariate analysis.}, doi = {10.5530/pj.2022.14.205}, author = {Wendy Nora Martian and Dini Kesuma and Rima Via Angraini} } @article {1776, title = {Formulation and Characterization of Meniran (Phyllanthus Niruri Linn) Extract Nanoparticle on Antibacterial Activity Against Salmonella Pullorum}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {April 2022}, pages = {369-373}, type = {Research Article }, chapter = {369}, abstract = {

This study aims to examine the results of meniran extract (Phyllanthus niruri Linn.) on antibacterial activity. Salmonella Pullorum is capable of causing huge economic losses. The misuse of antimicrobials has resulted in the evolution of multidrug-resistant strains. Meniran has potential as an antibacterial because it contains many bioactive components such as alkaloids, flavonoids, tannins and saponins. Nanoparticles help in the bioavailability of plant extracts. The research was conducted by making a meniran extract nanoparticles formulation with ionic glass method using chitosan and TPP sodium with a dose difference of 5\%, 10\% and 20\%. Each dose of meniran nanoparticles then were characterized by PSA, SEM and TEM. The result on PSA showed that size range from 192.67 nm to 385.16 nm and 5\% meniran extract nanoparticles have the best homogeneity and stability. EE value showed that the increase in the dose was directly proportional to the increase in the EE value. The result on SEM showed that the overall production of nanoparticle samples, it looks like they are nano-sized. The result on TEM showed small sample morphology with a good distribution. After that, the antibacterial activity test was then carried out using the MIC and MBC tests. The results showed that 5\% of meniran extract nanoparticles had the best antibacterial activity against Salmonella Pullorum.

}, keywords = {Nanoparticle, Phyllanthus niruri, Salmonella Pullorum}, doi = {10.5530/pj.2022.14.47}, author = {Sri Hidanah and Emy Koestanti Sabdoningrum and Sri Agus Sudjarwo} } @article {1744, title = {FTICR/MS Analysis of Micromeria Fruticosa and Teucrium Polium Growing in Lebanon}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {February 2022}, pages = {112-127}, type = {Original Article}, chapter = {112}, abstract = {

Micomeria fruticosa and Teucrium polium are Lamiaceae plants found throughout the Mediterranean, including Lebanon. Aerial parts of both plants were taken from a rocky mountain in Lebanon{\textquoteright}s Bekaa region and alcoholic extraction and chromatographic separation were performed. Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) was used to examine crude extracts of both plants A and B, as well as their fractions. The mass spectra of both plants revealed great chemical complexity with a total of 6352 ions (peaks) for crude extract of plant A and 5147 ions (peaks) for extract B. The majority of chemicals found in both plants are condensed aromatics, particularly polyphenols which could explain the previously documented anticancer effects of both plants.

Key words: Micromeria, Teucrium chromatographic analysis, FTICR-MS.

}, doi = {10.5530/pj.2022.14.16}, author = {Mohamad Al- Hamwi and MahaAboul Ela and Abdalla El-Lakany and Youssef BAKKOUR and Ziad Mahmoud} } @article {1860, title = {Functional Beverages from Blends of Ficus Deltoidea Leaves and Brown Rice Powders: Physico-Phytochemical Properties, Antioxidant Activities, Sensory Evaluation and Acute Toxicity Study}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {October 2022}, pages = {477-489}, type = {Original Article}, chapter = {477}, abstract = {

Background: Ficus deltoidea (Ficus: Moraceae) has great potential as a functional food. Administration of F. deltoidea has been reported to reduce hyperglycemia, oxidative stress and increase insulin secretion in diabetic rats and humans. However, the poor bioavailability and intestinal absorption of F. deltoidea impede its therapeutic effectiveness at a lower dosage, thus integrating F. deltoidea into brown rice will provide additional advantages. This study aimed to examine the phyto-physicochemical profile, antioxidant properties, consumer acceptance, and safety of beverages formulated from fine powder mixtures of F. deltoidea leaves and brown rice. Methods: The new beverage formulations were prepared by mixing the fine powders of F. deltoidea leaves with brown rice at ratios of 1:6 and 1:13, respectively. Physicochemical, phytochemical, and antioxidant analyses were performed to characterize the prepared beverages. Consumer acceptance was assessed utilising a 9-point hedonic scale and an acute toxicity study was employed to determine the safety of F. deltoidea-added formulations. Results: F. deltoidea decreased the pH and increased the moisture content, ash, and viscosity of a brown rice beverage. The total phenolic, flavonoid, and tannin content as well as antioxidant activities increased significantly in both F. deltoidea-added formulations. The oral LD50 of the F. deltoidea-added formulation was higher than 2000 mg/kg body weight. Conclusions: These results suggest that adding F. deltoidea leaves to brown rice beverages is safe to consume and improves the phyto-physicochemical profile, antioxidant activities, and consumers{\textquoteright} acceptance of the formulation.

}, keywords = {9-point hedonic scale., Animal study, DPPH assay, FRAP assay, Functional beverages}, doi = {10.5530/pj.2022.14.125}, author = {Nur Ain Sabrina Azmi and Nurdiana Samsulrizal and Siti Aimi Sarah Zainal Abidin and Noor Syaffinaz Noor Mohamad Zin and Norol Hamiza Zamzuri and Yong Meng Goh and Ana Sharmila Shafie and Rohaizad Abdul Raoof} } @article {1429, title = {Fenugreek (Trigonella Foenum-Graecum) and its Active Compounds: A Review of its Effects on Human Health through Modulating Biological Activities}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {May 2021}, pages = {813-821}, type = {Review Article}, chapter = {813}, abstract = {

Fenugreek seed are rich sources of various essential compounds and such components shows a significant role in the diseases cure and management through its anti-inflammatory, hepatoprotective, cardioprotective, neuroprotective, immunomodulatory, nephroprotective and anti-microbial activity. Moreover, fenugreek seed is a rich source of antioxidant and such potentiality inhibit the pathogenesis of diseases through free radical scavenging activity. Besides, fenugreek and its active compound shows role in tumour inhibition through modulating various genes activity including induction of apoptosis, tumour suppressor gene and inhibition of tumor necrosis factor. This review comprehensively summarizes the role of fenugreek seeds in health management through modulating various physiological and biochemical activities, along with special emphasis on in vitro and in vivo study.

}, keywords = {Anti-cancer activity, Anti-inflammatory, Anti-oxidant, Fenugreek, Health management, Trigonella foenum-graecum}, doi = {10.5530/pj.2021.13.103}, author = {Saleh A Almatroodi and Ahmad Almatroudi and Mohammed A Alsahli and Arshad Husain Rahmani} } @article {1406, title = {A Few Potential Treatments of Asthma in Vietnam}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {May 2021}, pages = {640-643}, type = {Original Article}, chapter = {640}, abstract = {

Asthma is a chronic condition that can be fatal if it isn{\textquoteright}t treated promptly during an attack. Patients with an asthma attack cannot breathe enough air to supply the body with oxygen and within minutes, the patient can die. In Vietnam today, people can buy antibiotics on their own without the need of a doctor to prescribe or visit. Many people who only see signs of coughing, wheezing have voluntarily bought antibiotics for use. The overuse of antibiotics is very common in asthma patients. In fact, antibiotics are not effective in most types of asthma. Abuse of antibiotics is not only ineffective, but also makes the patient more tired, reduces resistance, and digestive disorders. Some antibiotics can even lead to acute asthma attacks. This article covers some of the remedies that can eradicate asthma with a combination of several fairly safe treatments. Through analysis of the scientific composition and current studies, it is possible to treat asthma and maintain good health.

}, keywords = {Asthma, Health, Medicine, Patient, Prevention}, doi = {10.5530/pj.2021.13.81}, author = {Huynh Tan Hoi} } @article {1668, title = {Formulation and Characterization of Bitter Melon (Momordica charantia Linn.) Fruit Fraction Loaded Solid Lipid Nanoparticles}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {November 2021}, pages = {1347-1354}, type = {Original Article}, chapter = {1347}, abstract = {

Background: The main active compound of bitter melon (Momordica charantia Linn.) fruit is charantin, which is believed to have important role on antihyperglycemic effect. However, charantin compound has a large molecular weight and is easily hydrolysed when given orally. Therefore, a colloidal drug delivery system, such as solid lipid nanoparticles (SLN), is required to provide a suitable and effective delivery of charantin, which is contained in a bitter melon fraction (BMF). Objective: This study aimed to prepare and evaluate SLN containing BMF with an appropriate characteristic for transdermal delivery. Methods: Bitter melon fruits were extracted with ionic liquid of [BMIM]BF4 using ultrasound-assisted extraction (UAE) and fractionated with dichloromethane. Four formulas of BMF loaded SLN were prepared with various ratio of BMF to surfactant and various ratio of lipids using high-shear homogenization followed by ultrasonication method. The obtained SLN were characterized, including morphology, particle size distribution, zeta potential, and entrapment efficiency. Furthermore, the stability study of BMF-loaded SLN was also conducted. Results: The result showed that BMF was a dry powder and brownish fraction with a specific smell. The BMF loaded SLN showed a spherical shape with the SLN F1 formula as a selected formula. The SLN F1 showed a particle size (Z-average) of 98.3{\textpm}1.98 nm, polydispersity index of 0.26{\textpm}0.01, zeta potential of -39.53{\textpm}0.15 mV, and entrapment efficiency of 82.96{\textpm}1.42 \%. According to the stability study, it revealed that the BMF loaded SLN F1 had an acceptable stability, which the charantin content in the SLN was 96.52\% after 3 months storage at 25{\textdegree}C {\textpm} 2{\textdegree}C. Conclusion: The BMF loaded SLN F1 with 1:12 ratio of BMF to surfactant and 1:2 ratio of capric caprylic triglyceride to glyceryl monostearate was selected as the best formula with the appropriate characteristics for transdermal delivery.

}, keywords = {Bitter melon, Charantin, Momordica charantia Linn, Solid lipid nanoparticles}, doi = {10.5530/pj.2021.13.170}, author = {Rahayu Anggraini and Silvia Surini and Fadlina Chany Saputri} } @article {1718, title = {Formulation of Traditional Mask Powder Containing the Mixture of Coffea robusta, Angelica keiskei and Oryzae sativa, and its Activity as Tyrosinase Enzyme Inhibitor}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {December 2021}, pages = {1594-1597}, type = {Original Article}, chapter = {1594}, abstract = {

Objective: Formulate a traditional mask powder with the mixture of Coffea robusta (CR) green bean, Angelica keiskei (AK) leaf and Oryzae sativa (OS) and evaluate its activity as an inhibitor of the enzyme tyrosinase. Methods: The traditional mask powder was formulated by mixing homogeneously CR bean, AK leaf and OS, which is sieved by 125-μm sieve and sealed by foil aluminium. Subsequently, the traditional mask powder was evaluated including organoleptic, pH and microbial contamination test. The total phenolic content of CR bean, AK leaf and OS was determined by Folin-Ciocalteu test, and the inhibitory activities of the tyrosinase enzyme was determined using L-Tyrosine as substrate. Results: The physical stability of the traditional mask powder containing CR bean, AK leaf and OS was stable after 7 days of storage. The total phenolic content of CR bean, AK leaf and OS were 9.51, 2.80 and 2.2 gGAE/100g, respectively. The CR bean, AK leaf and OS had tyrosinase enzyme inhibitor activity with IC50 value 321.52, 930.10 and 339.55 μg/mL, respectively, while the IC50 of traditional mask powder was 127.60 μg/mL, and the IC50 kojic acid used as a positive control was 573.10 μg/mL. Conclusion: This study demonstrated that the traditional mask powder made the mixture of the CR bean, AK leaf and OS has a potential as a skin lightening agent.

}, keywords = {Angelica keiskei, Coffea robusta, Oryzae sativa, Traditional mask powder, Tyrosinase enzyme inhibitor.}, doi = {10.5530/pj.2021.13.205}, author = {Diah Lia Aulifa and Annisa Sri Rahayu Berliana and Nur Asni Setiani and Arif Budiman} } @article {1114, title = {Formulation and Evaluation of Safety and Antifungal Efficacy of Syzigium Aromaticum-Base Cream on Guinea Pigs Infected with Trichophyton Mentagrophytes}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {March 2020}, pages = {342-350}, type = {Research Article}, chapter = {342}, abstract = {

Background: The increasing incidence of dermatophytoses in the world and the side effects of the current therapies encouraged the search of alternative drugs. Hence the objective of this work was to determine antidermatophytes activity of Syzigium aromaticum formulate antidermatophytic cream. Materials and Methods: The extracts were prepared by maceration of plant materials into methanol. Three formulations of creams were made, and the best was chosen according to its physicochemical stability and appearance. The acute dermal toxicity and antidermatophytic efficacy of the cream was performed on guinea-pig. Results: The methanolic extract of S. aromaticum was incorporated in the final cream formulation. The formulation containing shea-butter 58.5\%, acetylic alcohol 2.5\%, stearic acid 1.5\%, bee-wax 10\%, borax 1.5\%, polysorbate 60 2.5\%, 2 drops of lactic acid and water was chosen because of its good appearance and stability. The cream with methanolic extract of S. aromaticum did not reveal any dermal toxic effect. The cream efficacy was dose-dependent. The treatment with cream at 5\% methanolic extracts of S. aromaticum revealed the best potency after 14 days of treatment. Conclusion: These results show that the cream at 5\% methanolic extract of S. aromaticum seed is promising in the treatment of dermatophytoses and could be used as an alternative in the development of a new therapy.

}, keywords = {Antidermatophytes activity, Cream, Dermatophytes, Formulation, S. aromaticum, Toxicity}, doi = {10.5530/pj.2020.12.54}, author = {Roger Ducos Youmsi Fokouo and Patrick Valere Tsouh Fokou and Cedric Derick Jiatsa Mbouna and Elisabeth Zeuko{\textquoteright}o Menkem and Fabrice Fekam Boyom} } @article {1195, title = {Formulation of Creams Containing Active Fraction of Cassia fistula L. Barks and its Antibacterial Activity against Propionibacterium acnes and Pseudomonas aeruginosa}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {June 2020}, pages = {920-928}, type = {Research Article}, chapter = {920}, abstract = {

Background: Cassia fistula L. has been traditionally used to cure skin diseases. That disease can be caused by various bacteria, such as Propionibacterium acnes and Pseudomonas aerugniosa. Objective: the objective of this research was to formulate cream containing active fraction of Cassia fistula bark and to study the antibacterial activity as well as physical stability of the active substance after formulation. Materials and Methods: The cream base were oilin- water (O/W) and water in oil (W/O) type. Antibacterial activity test had been performed by using agar diffusion method. Determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were conducted by microdilution method. The active fraction was formulated into creams with concentration of 4 -6x MIC. Physical evaluation of creams including organoleptic, pH, viscosity, TLC (Thin Layer Chromatography) profiling and antibacterial activity against both tested bacteria were evaluated during 28 days of storage. Results: The results showed that ethyl acetate fraction was the most active, having MIC and MBC values of 175 and 350 ppm respectively against P. acnes, while those against P. aeruginosa were 400 and 800 ppm. Optimation on creams using different type of cream bases showed that either O/W or W/O creams remained stable during 28 days of storage in terms of organoleptic and pH. The viscosity increased in O/W and decreased in W/O type. Qualitative analysis by TLC profiling showed that the ethyl acetate fraction of Cassia fistula as chemical compounds in creams was relatively stable as the profile remained the same after 28th day of storage. Result of antibacterial activity test on cream with O/W base was unchange after 28 day, while that with W/O revealed no activity which may due to poor diffusion within the cream base as media. Conclusion: active fraction of Cassia fistula can be formulated into cream with O/W cream base system.

}, keywords = {Antibacteria, Cassia fistula, Cream, Propionibacterium Acnes, Pseudomonas aeruginosa}, doi = {10.5530/pj.2020.12.131}, author = {Anis Yohana Chaerunisaa and Muhaimin Muhaimin and Yasmiwar Susilawati and Tiana Milanda} } @article {1153, title = {Fumaria indica (L), a Famous Medicinal Herb of Tribal Regions of Jabalpur, Madhya Pradesh: Broad Spectrum Antibacterial and Phytochemical Profilng Against Some Pathogenic Microorganisms}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {619-623}, type = {Research Article}, chapter = {619}, abstract = {

This work describes the broad spectrum antibacterial properties of methanolic and chloroform extracts of Fumaria indica herb in different concentrations (50 mg/ml, 100 mg/ml and 150 mg/ ml) against Bacillus subtilis(MTCC 10110), Staphylococcus aureus(MTCC96), Escherichia coli (MTCC 77), Pseudomonas aeruginosa (MTCC1688) and Klebsiella pneumonia (MTCC4032) using agar well diffusion method compared to standard antibiotic ciprofloxacin. Results have shown significant activities against the tested microorganisms viz., Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa than other strains. Minimum inhibitory as well as minimum bactericidal concentrations against Bacillus subtilis and Klebsiella pneumonia were evaluated. The study indicates the possible potentiality of F. indicato act as an active antibacterial agent in the modern drug formulations. As the target plant species serves for the tribal medicinal purpose in several tribal regions of Madhya Pradesh, hence, the aim of the present study is to link comparatively the possible traditional use of this herb with the modern antibiotic usage.

}, keywords = {Antibacterial activity, Fumaria indica, Phytochemicals, Tribes, Zone of Inhibition}, doi = {10.5530/pj.2020.12.92 }, author = {Shoket Ali and Shikha Bansal and Ravi Prakash Mishra} } @article {990, title = {Flavonoids in Passiflora incarnata L. Dry Extract of Russian Origin}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {September 2019}, pages = {1143-1147}, type = {Research Article}, chapter = {1143}, abstract = {

Background: Flavonoids are one of the main classes of biologically active substances providing the pharmacotherapeutic effect of passionflower (Passiflora incarnata L.) preparations. In this article studies on the standardization of Passiflora incarnata L. dry extract (PDE) by flavonoids are presented. The aim of this work was to study the composition and content of flavonoids in PDE with the help of precise modern physicochemical methods. Materials and Methods: PDE was prepared from crude herbal drug {\textendash} P. incarnata herb of Russian origin. Reverse phase HPLC-UV analysis was performed with Agilent 1100 liquid chromatograph. Chromatographic column was Atlantis C18 (250 mm {\texttimes} 4.6 mm {\texttimes} 5 μm); analytical wavelength {\textendash} 350 nm; mobile phase {\textendash} 0.01\% formic acid solution and methanol: acetonitrile (25:75); column temperature {\textendash} 35{\textdegree}C; analysis time {\textendash} 90 min; flow rate of the mobile phase {\textendash} 0.8 ml/min in gradient elution mode. Commercially available samples of flavonoids were used for identification and quantitative determination. Results: 20 compounds of flavonoid structure are presented in the PDE. 9 flavonoid compounds have been identified, they are: isovitexin, vitexin, rutoside, hyperoside, luteolin, kaempferol, kaempferitrin, orientin, and isoorientin. The content of vitexin is 0,867 {\textpm} 0,011\%, the total flavonoids content in terms of vitexin is 3,762 {\textpm} 0,049\%. Conclusion: The obtained data will be used to create regulatory documentation for drugs based on PDE.

}, keywords = {Dry extract, Flavonoids, Passiflora incarnata, standardization}, doi = {10.5530/pj.2019.11.178}, author = {Guseinov MD and Bobkova NV and Svistunov AA and Tarasov VV and Bokov DO and Sergunova EV and Kovaleva TYu} } @article {884, title = {Flavonoids of East Asian Species Serratula manshurica Kitag}, journal = {Pharmacognosy Journal}, year = {2019}, month = {March 2019}, pages = {xx-xx}, type = {Original Article}, chapter = {xx}, abstract = {

Aim/ Background: The flavonoid composition of the East Asian species Serratula manshurica Kitag. (Family Asteraceae) growing in the Primorsky Territory, the Amur region and in the South of the Khabarovsk Territory of Russia was studied. Materials and Methods: Flavonoids quercetin-4{\textquoteright}-O-β-D-glucuronopyranoside (1) and apigenin-7-O-β-D-glucuronopyranoside (2) were isolated from Serratula manshurica by methods of liquid extraction and recrystallization. The structures of the isolated compounds were identified by UV, NMR spectroscopy and mass spectrometry with electrospray ionization. Separation and quantitative content of flavonoids in the extracts of the aerial part (Leaves, stems, inflorescences) of Serratula manshurica was observed by RP HPLC. Results and Conclusion: The change in the amount of some flavonoids in various phenological phases in the overhead part of Serratula manshurica Kitag was studied. It was revealed that the maximum concentration of flavonoid aglycones in the plant was observed during the before budding in the budding period and their glycosides prevaily in the flowering phase. Widespread across the Far East, Serratula manshurica is promising and renewable source of bioactive compounds.

}, keywords = {Flavonoids, Leaves, NMR spectroscopy, Serratula manshurica, Steams}, doi = {10.5530/pj.2019.11.xx}, author = {Myagchilov Aleksey V and Sokolova Larisa I and Gorovoy Peter G and Dmitrenok Pavel S} } @article {1024, title = {Formulation and Characterization of Bitter Melon Extract (Momordica charantia) Loaded Phytosomes}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2019}, pages = {1235-1241}, type = {Original Article}, chapter = {1235}, abstract = {

Objective: Phytosomes are a novel drug delivery system that offers better absorption and bioavailability for extract or phytoconstituents. The aim of this study was developing bitter melon extract load phytosomes with appropriate characteristics for transdermal delivery. Methods: Three formulas were developed, F1, F2 and F3 with weight ratios of extract and phosphatidylcholine were 1: 1, 1: 2 and 1: 3, respectively. Bitter melon fruit was extracted using a maceration method and the marker compounds were determined by high performance liquid chromatography (HPLC) method. Phytosomes were prepared using thin layer method and then characterized, in terms of morphology, particle size distribution, zeta potential and entrapment efficiency. Results: The results of pytosomes characterization reveals that the F3 was the optimal formula. It has a spherical shape, particle size (DV-mean) was 282.3 {\textpm} 16.4 nm, zeta potential value at -39.2 {\textpm} 0.14 mV and entrapment efficiency of 90.06 {\textpm} 1.07 \%. Conclusion: Bitter melon extract loaded phytosomes with a weight ratio of extract and phosphatidylcholine 1:3 (F3) was selected as an optimal formula with appropriate characteristics for transdermal delivery.

}, keywords = {Bitter melon, Momordica charantia, Phytosomes, Thin layer method, Transdermal delivery}, doi = {10.5530/pj.2019.11.192}, author = {Rhatih Eka Sasongko and Silvia Surini and Fadlina Chany Saputri} } @article {1017, title = {Free Radical Scavenging Capacity and Total Phenolic Contents in Peel and Fleshy Crude Extracts of Selected Vegetables}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2019}, pages = {1351-1358}, type = {Research Article}, chapter = {1351}, abstract = {

Context: Vegetables are sources of natural antioxidants which have beneficial health-promoting properties. Aims: This research was conducted to evaluate the antioxidant potentials and total phenolic contents in vegetable extracts. Settings and Design: The experiment was divided two groups using six vegetable peels and fleshes, including potato (Solanum tuberosum L.), carrot (Daucus carota L.), bitter gourd (Monordica Charantia L.), pumpkin (Cucurbita moschana Duchesn), Chinese radish (Raphanus sativus L.), and cucumber (Cucumis sativus L.). Materials and Methods: The samples were extracted with 95\% ethanol for 24 hrs and then analyzed the antioxidant activities by DPPH and FRAP assay and total phenolic contents was determined by Folin-Ciocalteu assay. Statistical analysis used: All the analysis was done in triplicate, and values are represented as means {\textpm} standard deviations. The data were subjected to one-way analysis of variance (ANOVA) and significance differences between mean values were determined by Tukey{\textquoteright}s multiple comparison tests (p \< 0.05). Pearson correlation coefficient was determined between the antioxidant activities and total phenolic contents using SPSS version 21. Results: The results revealed that antioxidant activities were different statistically significant in each group (p \< 0.05). Carrot peel was the most potent in DPPH radical scavenging activity (61.80 {\textpm} 6.14\%) and reducing power (0.75 {\textpm} 0.25 mM Fe2+/g extract) and potato flesh could inhibit DPPH radical (35.60 {\textpm} 0.63 \%) and had the highest FRAP value (0.61 {\textpm} 0.78 mM Fe2+/g extract). Pumpkin peel and Chinese radish flesh had the most total phenolic contents as 110.45 {\textpm} 22.49 and 142.15 {\textpm} 3.53 mg GAE/g extract, respectively. Although no correlations was between antioxidant activities and total phenolic contents in extracts but were significant correlations between DPPH and FRAP assay in vegetable peels and fleshes as 0.931 and 0.819, respectively. Conclusion: This study substantiates the potential of vegetable peels to be used as functional foods and natural free radical scavengers.

}, keywords = {Antioxidants, Free Radicals, Total Phenolic Contents, Vegetable fleshes, Vegetable peels}, doi = {10.5530/pj.2019.11.209}, author = {Chanate Wanna} } @article {908, title = {Free Radicals Scavenging Activity and Oxidative DNA Damage Protecting Property of Methanol Extract from Honeycrisp Apple}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {July 2019}, pages = {694-698}, type = {Original Article}, chapter = {694}, abstract = {

Objectives: This research reports the qualitative phytochemical constituents and considers the in vitro free radicals scavenging activities based on DPPH and nitric oxide assays and oxidative DNA damage protection activity of methanol extract from Honeycrisp apple. The foremost interest for this research was to use standard measures to determine nitric oxide scavenging activity, DPPH-scavenging activity and DNA damage protecting activity to assess the antioxidant potential of methanol extract from the apple. Materials and Methods: Concentrations of apple extracts with the intervals 20 μg/ml to 100 μmg/ml were prepared and mixed with suitable volumes of reagents and the corresponding absorbances read at the respective wavelength. Results: The outcomes of this research specify that methanol extract of Honeycrisp apple contain multiple phytochemical compounds that can expertly shield the body against oxidative stress caused by free radicals and might then be used as a source of potent natural antioxidant compounds. The IC50 values of methanol extract from Honeycrisp apple varied from 10.30 to 36.29 μg/ml which indicates the high radical scavenging activity of the sample. Conclusion: The DNA damage defensive potential of the extracts was also revealed, which could be used in cancer inhibition.

}, keywords = {DNA damaging, DPPH, Free Radicals, Honeycrisp apple, Nitric oxide, Phytochemicals}, doi = {10.5530/pj.2019.11.110}, author = {Williams Kweku Darkwah and Matthew Nkoom} } @article {600, title = {Ficus religiosa Tree Leaves as Bioindicators of Heavy Metals in Gorakhpur City, Uttar Pradesh, India}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {March 2018}, pages = {416-420}, type = {Original Article}, chapter = {416}, abstract = {

Objective: The present work investigates the metal uptake of the plant Ficus religiosa leaves collected from nine experimental sites in Gorakhpur, Uttar Pradesh. Materials and Methods: The concentration of lead in leaves was determined with help of atomic absorption spectrophotometer. Results: The level of the lead in leaves of Ficus religiosa was much higher than the limits recommended by World Health Organization (WHO). The bioaccumulation of lead was highest in site 2 (Gayatri nagar) i.e. 14.1 mg kg-1 dry weight of F. religiosa. To determine the relationship between the lead concentration in leaves and traffic density the Pearson\’s correlation analysis has been performed. Conclusion: The value of Pearson\’s correlation coefficient was positive indicating that the traffic density plays an important role in the lead pollution in plants.

}, keywords = {Atomic absorption spectroscopy, Lead, Metal}, doi = {10.5530/pj.2018.3.68}, url = {http://fulltxt.org/article/501}, author = {Pooja Agrahari and Richa R and Kumari Swati and Supriya Rai and Vinay Kumar Singh and Dinesh Kumar Singh} } @article {714, title = {Formulation and Evaluation of Solid Dispersion Tablet of Andrographis paniculata Extract}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {1047-1054}, type = {Original Article}, chapter = {1047}, abstract = {

Objective: To enhance solubility of ethanolic extract of Andrographis paniculata by solid dispersion technique and to perform formulation and evaluation of solid dispersion tablet. Materials and Methods: Solid dispersion of Andrographis paniculata extract has been prepared by solvent evaporation technique using soluplus and PEG 6000. Prepared solid dispersions have been evaluated for various micromeritic properties. The tablets of solid dispersion were prepared by direct compression technique and were evaluated for various physical tests and in-vitro dissolution study. Results: The study showed that prepared solid dispersion has good flow property and compressibility. The solubility of extract was found to be more from solid dispersion prepared by using soluplus than that of prepared by using PEG 6000. The rate of drug release was found to be higher in acidic buffer at pH 1.2 as compared to that of in phosphate buffer at pH 6.8. Conclusion: The study concludes that the solid dispersion tablet of ethanolic extract of Andrographis paniculata can be effectively prepared using soluplus by solvent evaporation techniqu

}, keywords = {Andrographis paniculata, Ethanolic extract, PEG 6000, Solid dispersion, Soluplus, Solvent evaporation technique}, doi = {10.5530/pj.2018.5.177}, author = {Sachin Annasaheb Nitave and Nilesh B. Chougule and Kailasam Koumaravelou} } @article {606, title = {Functional and Morphological Studies of Organic and Inorganic Tomatoes}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {May 2018}, pages = {715-719}, type = {Original Article}, chapter = {715}, abstract = {

Aim: To study the functional and morphological characteristics of organic and inorganic tomatoes. Methodology: UV treated, and untreated organic and inorganic tomato powdered samples were assessed using an analytical technique FT-IR and SEM. Results: FT-IR spectra of organic control samples showed peaks at (3290.12, 2924.72 and 1611.61 cm-1) while, organic treated samples revealed some peaks at (3287.11, 2925.66 and 1612.91 cm-1), inorganic control tomatoes confirmed the peaks at (3289.47, 2925.76 and 1619.26 cm-1) and in inorganic treated tomatoes peaks was at (3289.42, 2925.34 and 1619.31 cm-1) respectively, on confirming the presence of O-H, C-H, C=C, C=O and C-OH functional groups. However, results confirmed slight changes in functional groups of organic and inorganic samples and UV treatment helps in decreasing the effect of fluoride, chloride and other synthetic chemicals level by disinfecting the samples. SEM micrographs showed non-significant changes with minimal effect of UV in all samples. Conclusion: The outcome of the study helps in effective understanding of functional and microstructural differences between organic and inorganic tomatoes.

}, keywords = {FT-IR, Inorganic, Organic, SEM, Tomatoes, UV treatment}, doi = {10.5530/pj.2018.4.119}, url = {http://fulltxt.org/article/657}, author = {Shwetha M S and Sinija V R and M Durgadevi and B K Yadav and S Shanmugasundaram} } @article {632, title = {Functional and Morphological Studies of Organic and Inorganic Tomatoes}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {June 2018}, pages = {715-719}, type = {Original Article}, chapter = {715}, abstract = {

Aim: To study the functional and morphological characteristics of organic and inorganic tomatoes. Methodology: UV treated, and untreated organic and inorganic tomato powdered samples were assessed using an analytical technique FT-IR and SEM. Results: FT-IR spectra of organic control samples showed peaks at (3290.12, 2924.72 and 1611.61 cm-1) while, organic treated samples revealed some peaks at (3287.11, 2925.66 and 1612.91 cm-1), inorganic control tomatoes confirmed the peaks at (3289.47, 2925.76 and 1619.26 cm-1) and in inorganic treated tomatoes peaks was at (3289.42, 2925.34 and 1619.31 cm-1) respectively, on confirming the presence of O-H, C-H, C=C, C=O and C-OH functional groups. However, results confirmed slight changes in functional groups of organic and inorganic samples and UV treatment helps in decreasing the effect of fluoride, chloride and other synthetic chemicals level by disinfecting the samples. SEM micrographs showed non-significant changes with minimal effect of UV in all samples. Conclusion: The outcome of the study helps in effective understanding of functional and microstructural differences between organic and inorganic tomatoes.

}, keywords = {FT-IR, Inorganic, Organic, SEM, Tomatoes, UV treatment}, doi = {10.5530/pj.2018.4.119}, url = {http://fulltxt.org/article/657}, author = {Shwetha Manchanabele Shankar and Sinija Vadakkepulppara Ramachandran Nair and Manoharan Duragdevi and Binod Kumar Yadav and Shanmugasundaram Saravanan} } @article {728, title = {Functional Ingredients and Medicinal Prospects of Ethanol Extract from Macrocybe lobayensis}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {1154-1158}, type = {Original Article}, chapter = {1154}, abstract = {

Introduction: Mushrooms are a good source of bioactive components and have been traditionally consumed across the globe for maintaining health. However, information concerning medicinal activity of ethnically popular macrofungi still remains limited. Aim: Therefore, the present study was aimed for determination of functional constituents and therapeutic efficacy of a less explored mushroom, Macrocybe lobayensis that has been habitually used in local diets. Method: In this context, an ethanol extract was prepared from dried basidiocarps and characterized by spectrophotometry and HPLC to identify bioactive constituents. Alongside, the fraction was also subjected for evaluation of medicinal properties namely antioxidant and antibacterial effects. Results: The formulation was found to be consisted mainly of phenolic compounds (pyrogallol\> cinnamic acid\> p-coumaric acid\> p-hydroxybenzoic acid). As a result, the fraction exhibited strong antioxidant activity evident by the ability of quenching DPPH and ABTS radicals, chelating capacity of Fe2+ as well as reducing components with EC50 of 1000 to 2264 \μg/ml. Besides, the formulation also emerged as a potent source of antibiotic as it inhibited growth of investigating microbes in order of Staphylococcus aureus\> Escherichia coli\> Bacillus subtilis\> Salmonella typhimurium\> Listeria monocytogenes. Conclusion: Thus, the outcome might encourage use of M. lobayensis as natural antioxidant and antibacterial agent in pharmaceutical, cosmetic or food industries.

}, keywords = {Antibacterial activity, Antioxidant potential, Ethanol Extract, Wild edible mushroom}, doi = {10.5530/pj.2018.6.197}, author = {Somanjana Khatua and Krishnendu Acharya} } @article {322, title = {Fasting Blood Glucose Levels and Hematological Values in Normal and Streptozotocin-Induced Diabetic Rats of Mimosa pudica L. Extracts}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {April 2017}, pages = {315-322}, type = {Original Article}, chapter = {315}, abstract = {

Context:M. pudica is a common plant found in moist waste ground, lawns, open plantations and weedy thickets. Aims: The fasting blood glucose levels (FBG) and hematological values of M. pudica aqueous(MPA) and hydro-ethanolic (MPHE) extract were evaluated in normal and streptozotocin (STZ)-induced diabetic rats. Materials and Methods: MPA and MPHE 125, 250 and 500 mg/kg body weight (b.w.) were administered orally and daily to the rats for 8 weeks. The FBG were determined weekly. Red blood cells (RBC), hemoglobin (HM. pudicab), hematocrit (Hct), platelet, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cells (WBC), lymphocytes, monocytes, neutrophils and eosinophil were evaluated. Results: MPA and MPHE had no effect on blood glucose levels in normal rats. All doses of all extracts showed significantly (p\<0.05) decreasing FBG in diabetic rats. Especially MPA at the dose of 250 mg/kg b.w. showed more potent significantly (p\<0.05) decreasing blood glucose levels than anti-diabetic drug glibenclamide at the end of experiment. All extracts had no effect on RBC, Hb, Hct, platelet, MCH, MCHC, lymphocytes, monocytes neutrophils and eosinophils. Surprisingly, the extracts were decreased WBC and MCV in diabetic rats. In addition, all of the extracts did not produce the alteration of blood cells structure in all rats. Conclusion: This study indicated that the extracts were hypoglycemic effect and improve hematological values in diabetes which confirms the traditional use of the plant.

}, keywords = {Blood glucose level, Hematological values, Mimosa pudica, Red blood cell, White blood cell}, doi = {10.5530/pj.2017.3.54}, url = {/files/PJ-9-3/10.5530pj.2017.3.54}, author = {Ampa Konsue and Chayan Picheansoonthon and Chusri Talubmook} } @article {451, title = {Formulation Development and Evaluation of Anti-inflammatory Potential of Cordia obliqua Topical Gel on Animal Model}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {November 2017}, pages = {s93-s98}, type = {Original Article}, chapter = {s93}, abstract = {

Background: The plants of genus Cordia has been used in traditional medicine for many diseases from history. Cordia obliqua Willd. is also a medicinally important plant and it has been proved by modern scientific research. Objective: This study is aimed to formulate and characterize a topical gel from Cordia obliqua leaf methanol extract and evaluate its effect on carrageenan induced rat paw edema. Materials and Methods: The methanol extract was prepared by successive solvent extraction using soxhlet apparatus. Topical gel was designed to prepare by Dispersion method using Carbopol 940 as polymer. The various gel characteristics was studied by standard procedures like Homogeneity, grittiness, extrudability, drug content, spread ability and in-vitro diffusion study. The anti-inflammatory potential of gel was evaluated by Carrageenan induced rat paw edema method in comparison with standard Diclofenac Sodium gel 1\% on Albino rats. Results: The herbal gel was found homogeneous with good extrudability, no grittiness, pH 6.79 and drug content 93.167\%. The anti-inflammatory activity of herbal gel was found comparable with standard Diclofenac Sodium gel and has shown 82.71\% edema inhibition after 4 h of treatment. Conclusion: Cordia obliqua gel was found suitable as a standard topical gel formulation and it can be used safely for treatment of edema.

}, keywords = {Carbopol 940, Carrageenan induced edema, Cordia, in-vitro diffusion, Topical gel}, doi = {10.5530/pj.2017.6s.163}, url = {http://fulltxt.org/article/388}, author = {Richa Gupta and Ghanshyam Das Gupta} } @article {348, title = {Fractionation and α-glucosidase Inhibitory Activity of Fractions from Garcinia hombroniana Pierre Leaves Extracts}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {May 2017}, pages = {488-492}, type = {Original Article}, chapter = {488}, abstract = {

Background: Diabetes mellitus become one of the biggest global health problems of the 21st century. Type 2 diabetes play role for the majority of cases of diabetes worldwide which is characterized by the increase of postprandial blood glucose level. Maintaining postprandial glucose level through inhibition of \α-glucosidase is one of the essential strategies in the treatment of diabetes. Inhibitory effect of \α-glucosidase was commonly used to identify active compounds potentially to treat diabetes. Natural resources have potency as antidiabetic that can be used in diabetes treatment. Objective: The objective of the study is to separate active fraction in the crude extract of Garcinia hombroniana leaves to facilitate obtaining a pure biologically active compound as the \α-glucosidase inhibitor. Methods: Fractionation to separate active fraction was performed using column and thin layer chromatography methods while \α-glucosidase inhibitory activity assay was performed in vitro using spectrophotometric methods at \λ 400 nm. Results: Ethyl acetate and methanol extract of G. hombroniana yielded 14 and 12 fractions, respectively. Two fractions with the higher percent inhibition compared to other factions are fraction 8 from ethyl acetate extract (FEA8) and fraction 3 from methanol extract (FM3). The IC50 values of FEA8, FM3 and acarbose are 16.370 \μg/mL, 59.042 \μg/mL, and 39.534 \μg/mL respectively. Conclusion: Fraction 8 from ethyl acetate extract of G. hombroniana leaves (FEA8) was separated and known in this study as the most bioactive \α-glucosidase inhibitor agent compared with another extract, fractions, and acarbose.

}, keywords = {Column chromatography, Diabetes mellitus, Fractionation, Thin layer Chromatography, α-glucosidase}, doi = {10.5530/pj.2017.4.79}, url = {/files/PJ-9-4/10.5530pj.2017.4.79}, author = {Nita Triadisti and Rani Sauriasari and Berna Elya} } @article {125, title = {Finger Printing of the Anatomical Markers, HPTLC Profile and Heavy Metals Content in the Leaves of Aristolochia indica Linn.}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {December 2015}, pages = {117-126}, type = {Original Article}, chapter = {117}, abstract = {

Background: Aristolochia indica Linn is now an endangered medicinal plant belonging to the family Aristolochiaceae. Many ethono-botanically important species of Aristolochia were found used in the traditional forms of medicine for the treatment of various illnesses. Aim: The present study concerns the microscopic, fluorescent, powder, quantitative microscopic characteristics of the leaves of Aristolichia indica Linn and its physico-chemical standards. Materials and Methods: Morpho-histological profile, Highperformance thin-layer chromatographic (HPTLC) finger print profile, and heavy metals content of the leaves of Aristolichia indica Linn. Results: The presence of camptodromous pinnate venation with pentagonal shaped areoles with linear veinlet endings, non-glandular hooked trichomes, amphistomatic and anomocytic stomata, small palisade ratio, small stomatal index were the anatomical features characteristics of the taxon. Physico-chemical evaluation of the leaves gave moisture content of 10.5\%, total ash 12.7\%, acid insoluble ash 1.9\%, acid soluble ash 10.8\%. Highperformance thin-layer chromatographic (HPTLC) identification of marker compound (Aristolochic acid I or (AAI)) in methanol extract of leaves was carried out. The developed HPTLC Chromatogram had revealed nine phytoconstutents in extract of leaf sample. The Rf value for Aristolochic acid I (C17H11NO7) was found to be 0.41 and densitometric scanning had shown \λmax at 318 nm for the marker compound. The content of marker constituent (AA I) present in shade-dried leaves of Aristochia indica Linn was estimated as 0.049\%. The Flame atomic absorption spectrometric determination of elements had shown appreciable amounts of the elements such as Fe (0.5442 mg/g), Zn (0.026.82 mg/g), Ni (0.008 mg/g,) and Cu (0.002 mg/g) by dry weight of the leaves of Aristolichia indica. Conclusion: The present study suggests that the delineated characteristics of the leaves of A. indica, could tag as the identifying parameters to substantiate and authenticate the raw drugs from the spurious/adulterants materials and could also be effectively used for the regulatory perspectives and quality assessment of Aristololic acid \Ι in the medicinal formulation/finished products. The presence of important mineral elements inside this plant showed that it could be a nutritious plant and important to the human health.

}, keywords = {Areoles, Aristolic acid, Heavy metals., HPTLC finger print, Physico-chemical parameters, Stomatal index}, doi = {10.5530/pj.2016.2.4}, author = {Madathilparambil Vasu Sudhakaran} } @article {152, title = {Fingerprint Of Tiger Balm{\textregistered} By Thermal Desorption Gas Chromatography Mass Spectroscopy}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {February 2016}, pages = {230-233}, type = {Original Article}, chapter = {230}, abstract = {

Introduction: Tiger Balmis a blend of volatile oils used for many years in control of muscle cramps and headache. Objectives: To establish a quick and accurate method of analysis for Tiger Balm. Materials and Methods: GC-MS and TD GC-MS were used in parallel to define the metabolites available in Tiger Balm blend, high quality standards were help in confirmation of the metabolites identities. Results: TD GC-MS was more\ efficient in showing the 1:1 relative abundance of camphor and menthol\ which can be taken as a chemical marker of this herbal medicine. Conclusion:\ In this work we applied efficiently the use of TD GC-MS in quality\ analysis of semisolid herbal medicine with volatile scents without the\ need of tedious pre-treatment with organic solvents, which is required\ by using GC-MS.

}, keywords = {Camphor, GC-MS, Menthol., TD GC-MS, Tiger Balm{\textregistered}}, doi = {10.5530/pj.2016.3.9}, author = {Omar A. Aldulaimi and Wen W. Li} } @article {1478, title = {Flavonoids from Eupatorium illitum and Their Antiproliferative Activities}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {09th Mar, 2015}, pages = {178-181}, type = {Original Article}, chapter = {178}, abstract = {

Objective: To isolate the chemical constituents of the aerial parts from Eupatorium illitum, elucidate their structures and evaluate their antiproliferative activity on human cancer cell lines. Materials and Methods: The ethanolic extract of Eupatorium illitum afforded five compounds, which were characterized using spectroscopic techniques and by comparison with data from the literature. Antiproliferative activities of selected isolates were evaluated. Results: The flavonoids Kumatakenin (1), Ermanin (2), 7-methoxy-aromadendrin (3) and Naringenin (4), together with 4-hydroxybenzoic acid (5) were isolated. Compounds 1, 2, 4 and 5 were evaluated for their antiproliferative activity on the human cancer cell lines A549 (lung), HBL-100 (breast), HeLa (cervix), SW1573 (lung), and T-47D (breast) presenting a wide range of bioactivities. In general, best results were observed for 5. Conclusion: Compounds 1-5 are reported for first time from Eupatorium illitum. Isolated phytochemicals show moderate to low antiproliferative activities when evaluated on the aforementioned human cancer cell lines.

Key words: Eupatorium illitum, Ermanin, 4-Hydroxybenzoic acid, Kumatakenin, 7-Methoxy-aromadendrin, Naringenin.

}, keywords = {4-Hydroxybenzoic acid, 7-Methoxy-aromadendrin, Ermanin, Eupatorium illitum, Kumatakenin, Naringenin.}, author = {Qu{\'\i}rico A Castillo and Jorge Triana and Jos{\'e} L Eiroa and Jos{\'e} M Padr{\'o}n and Gabriela B Plata and Ernesto V Abel-Santos and Luis A. B{\'a}ez and Diana C Rodr{\'\i}guez and Marco A Jim{\'e}nez and Mar{\'\i}a F P{\'e}rez-Pujols} } @article {51, title = {Flavonoids from Eupatorium illitum and Their Antiproliferative Activities}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {01/2015}, pages = {178-181}, type = {Original Article}, chapter = {178}, abstract = {

Objective: To isolate the chemical constituents of the aerial parts from Eupatorium illitum, elucidate their structures and evaluate their antiproliferative activity on human cancer cell lines. Materials and Methods: The ethanolic extract of Eupatorium illitum afforded five compounds, which were characterized using spectroscopic techniques and by comparison with data from the literature. Antiproliferative activities of selected isolates were evaluated. Results: The flavonoids Kumatakenin (1), Ermanin (2), 7-methoxy-aromadendrin (3) and Naringenin (4), together with 4-hydroxybenzoic acid (5) were isolated. Compounds 1, 2, 4 and 5 were evaluated for their antiproliferative activity on the human cancer cell lines A549 (lung), HBL-100 (breast), HeLa (cervix), SW1573 (lung), and T-47D (breast) presenting a wide range of bioactivities. In general, best results were observed for 5. Conclusion: Compounds 1-5 are reported for first time from Eupatorium illitum. Isolated phytochemicals show moderate to low antiproliferative activities when evaluated on the aforementioned human cancer cell lines.

}, keywords = {4-Hydroxybenzoic acid, 7-Methoxy-aromadendrin, Ermanin, Eupatorium illitum, Kumatakenin, Naringenin.}, doi = {10.5530/pj.2015.3.6}, author = {Qu{\'\i}rico A Castillo and Jorge Triana and Jos{\'e} L Eiroa and Jos{\'e} M Padr{\'o}n and Gabriela B Plata and Ernesto V Abel-Santos and Luis A. B{\'a}ez and Diana C Rodr{\'\i}guez and Marco A Jim{\'e}nez and Mar{\'\i}a F P{\'e}rez-Pujols} } @article {55, title = {Free Radical Scavenging and Cytotoxic Potential of Celosia argentea}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {01/2015}, pages = {191-197}, type = {Original Article}, chapter = {191}, abstract = {

Introduction: Oxidative stress due to reactive oxygen species often leads to pathogenesis of chronic diseases such as cancer. Research states that a diet rich in polyphenols renders many health benefits by scavenging such harmful reactive species. Celosia argentea (Amaranthaceae), a common weed in India has been reported as a potential source of cheap, natural antioxidants due to its phenolic abundance. In this research work efforts were made to identify and screen the phenolic rich fractions of Celosia argentea for their antioxidant and anticancer potential. Materials and Methods: Various solvent fractions with increasing polarity were subjected to total phenolic content, followed by antioxidant assays-DPPH, ABTS and anti proliferative assays- Brine shrimp Bioassay, Antimitotic and MTT assays. Results: IC50 value of methanolic fraction for DPPH assay was statistically significant (26.25; ***P\<0.001) when compared with ascorbic acid (12.50; ***P\<0.001). Also TEAC values for methanolic fraction and BHT (standard) for ABTS assay were similar (2.1; ***P\<0.001) Methanolicfraction at 400 \μg/ml exhibited strong cytotoxicity (9.0 \± 0.81; ***P\<0.001) against brine shrimps comparable to Methotrexate at 50 \μg/ml(10; ***P\<0.001) and significantly reduced mitotic index from 96.8 to 38.0 (***P\<0.001) which was further confirmed by MTT assay where IC50 value of methanolic fraction for SiHa and MCF-7 cells was found to be 28 \μg/ml with no cytotoxicity to normal cells proving its anticancer potential. Conclusion: This research proves antioxidant and anticancer potential of phenolic rich fraction of Celosia argentea and suggests it to be useful in cancer management as antifroliferative, chemo preventive and in cancer chemotherapy induced immune suppression and oxidative stress.

}, keywords = {Antioxidant, Brine shrimp, Mitotic index, MTT, Phenolics.}, doi = {10.5530/pj.2015.3.8}, author = {Rukhsana Abdul Rub and Manohar Janardhan Patil and Areej Abdul Siddiqui and Priyanka Rambhau Ghorpade and Alpana Subhod Moghe} } @article {1479, title = {Free Radical Scavenging and Cytotoxic Potential of Celosia argentea}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {09th Mar, 2015}, pages = {191-197}, type = {Original Article}, chapter = {191}, abstract = {

Introduction: Oxidative stress due to reactive oxygen species often leads to pathogenesis of chronic diseases such as cancer. Research states that a diet rich in polyphenols renders many health benefits by scavenging such harmful reactive species. Celosia argentea (Amaranthaceae), a common weed in India has been reported as a potential source of cheap, natural antioxidants due to its phenolic abundance. In this research work efforts were made to identify and screen the phenolic rich fractions of Celosia argentea for their antioxidant and anticancer potential. Materials and Methods: Various solvent fractions with increasing polarity were subjected to total phenolic content, followed by antioxidant assays- DPPH, ABTS and anti proliferative assays- Brine shrimp Bioassay, Antimitotic and MTT assays. Results: IC50 value of methanolic fraction for DPPH assay was statistically significant (26.25; ***P\<0.001) when compared with ascorbic acid (12.50; ***P\<0.001). Also TEAC values for methanolic fraction and BHT (standard) for ABTS assay were similar (2.1; ***P\<0.001) Methanolicfraction at 400 \μg/ml exhibited strong cytotoxicity (9.0 \± 0.81; ***P\<0.001) against brine shrimps comparable to Methotrexate at 50 \μg/ml(10; ***P\<0.001) and significantly reduced mitotic index from 96.8 to 38.0 (***P\<0.001) which was further confirmed by MTT assay where IC50 value of methanolic fraction for SiHa and MCF-7 cells was found to be 28 \μg/ml with no cytotoxicity to normal cells proving its anticancer potential. Conclusion: This research proves antioxidant and anticancer potential of phenolic rich fraction of Celosia argentea and suggests it to be useful in cancer management as antifroliferative, chemo preventive and in cancer chemotherapy induced immune suppression and oxidative stress.

Key words: Antioxidant, Brine shrimp, Mitotic index, MTT, Phenolics.

}, keywords = {Antioxidant, Brine shrimp, Mitotic index, MTT, Phenolics.}, author = {Rukhsana Abdul Rub and Manohar Janardhan Patil and Areej Abdul Siddiqui and Priyanka Rambhau Ghorpade and Alpana Subhod Moghe} }