02330nas a2200217 4500008004100000245016000041210006900201260001700270300001400287490000700301520160000308653002001908653002301928653003101951653001901982653002902001100002102030700002002051700000502071856003602076 2019 eng d00aEffects of Beta Glucosidase Inhibitor on Cellulase Enzyme Activity for Preparation of Microcrystalline Cellulose from Water Hyacinth (Eichhornia crassipes)0 aEffects of Beta Glucosidase Inhibitor on Cellulase Enzyme Activi cOctober 2019 a1225-12300 v113 a

Objective: Microcrystalline cellulose (MCC) was a highly desirable excipient which being used for making tablets with direct compression method in Pharmaceutical manufacture. The purpose of this study was to compare microcrystalline cellulose preparation from water hyacinth powder with and without addition of β-glucosidase inhibitor followed by identification and characterization of the resulting powders. Methods: The study was began with isolation of potential organisms from soils in mangrove followed by extraction of • -glucosidase inhibitor. MCC was prepared through enzymatic hydrolysis of alpha-cellulase with and without addition of beta-glucosidase inhibitor. Identification was done using FTIR, then characterized by organoleptic examination, qualitative analysis, starch test, pH test, Scanning Electron Microscopy (SEM) analysis of particle size and distribution, X-ray Diffraction (XRD), moisture content, loss on drying test, particle density test, flow rate test and angle of repose test compared to microcrystalline cellulose which had been available on the market. Results: The hydrolysis conditions were carried out at 30°C, for 2 h and the powder was dissolved in acetate buffer pH 7 by addition of enzyme and 2.5 ml inhibitor. MCC yield with addition of beta-glucosidase inhibitor (80%) was higher than without addition of beta-glucosidase (68%). Conclusion: Addition of beta glucosidase inhibitor showed positive impact to increase MCC yield from alpha cellulose sample.

10aCharaterization10aEnzyme hydrolyisis10aMicrocrystalline cellulose10aWater hyacinth10aβ-glucosidase inhibitor1 aShabrina, Annisa1 aSuryadi, Herman1 a uhttps://phcogj.com/article/1022