02206nas a2200229 4500008004100000245015800041210006900199260001700268300001400285490000700299520144200306653002301748653001301771653002201784653001401806653002801820653002701848100002701875700001601902700002201918856003601940 2019 eng d00aInhibitory Effects of Sangketan (Achyranthes aspera L.) Roots Extract on Arginase Activity and Determination of Its Total Phenolic and Flavonoid Contents0 aInhibitory Effects of Sangketan Achyranthes aspera L Roots Extra cOctober 2019 a1231-12340 v113 a

Background: Achyranthes aspera, or commonly called as Sangketan in Indonesian is a wild plant that is used as a traditional medicine. The roots of Sangketan can be used as a wound healer by involving the role of arginine and its metabolites, nitric oxide, that directly affect the wound healing process itself. Objective: The aim of this study is to determine the potential of Sangketan roots extract in inhibiting arginase activity. Methods: The roots were extracted using multistage ultrasound-assisted extraction method with n-hexane, ethyl acetate and methanol solvent. Each extract from different solvents was tested for the inhibition of arginase activity using a microplate-based colorimetric method, followed by determination of total phenolic concentration and total flavonoid concentration. Results: The results of inhibition test of arginase activity by n-hexane, ethyl acetate and methanolic extracts were 9.56; 17.58; and 29.77% sequentially/respectively at concentration of 100 μg/ml; the total phenolic concentration were 3.91; 4.83; dan 11.18 mgGAE/g of sample respectively; and the total flavonoid concentration are 0.29; 0.80; and 0.88 mgQE/g of sample respectively. Conclusion: From this research, it can be concluded that Sangketan roots extract had low potency of arginase inhibitory activity.

10aAchyranthes aspera10aArginase10aInhibitory effect10aSangketan10atotal flavonoid content10atotal phenolic content1 aRahmawati, Dieah, Siti1 aElya, Berna1 aNoviani, Arikadia uhttps://phcogj.com/article/1023