02074nas a2200217 4500008004100000245010900041210006900150260001800219300001400237490000700251520139800258653002201656653001601678653002601694100001801720700002101738700002101759700001901780700002101799856003601820 2019 eng d00aTwo Triterpenoid Saponins with alpha-glucosidase Inhibitory Activity from Harpullia pendula Seed Extract0 aTwo Triterpenoid Saponins with alphaglucosidase Inhibitory Activ cNovember 2019 a1386-13900 v113 a

Background: Harpullia pendula Planch (family Sapindaceae) is a small to medium rainforest tree native to Australia. Objective: This study aims to isolate triterpenoid saponins from H. pendula and test them as α-glucosidase inhibitors. Materials and Methods: The saponin compounds were obtained using variable chromatographic techniques and characterized by spectral analysis. Results: Two new triterpenoid saponins were obtained as an inseparable mixture from H. pendula methanolic seed extract. Their structures were determined as 3-O-β-D-glucopyranosyl-(1→2)-[α-L-arabinofuranosyl-(1→3)]-βD-glucuronopyranosyl22-OangeloylA1- barrigenol and 3-O-β-D-galactopyranosyl-(1→2)-[α-L-arabinofuranosyl-(1→3)]-β-Dglucuronopyranosyl 22-O-(2-methylbutyroyl)-A1 barrigenol, respectively. The triterpene part 22-O-(2-methyl butyroyl) A1-barrigenol has never been characterized before. The α- glucosidase inhibitory activity of the two saponin mixture was evaluated invitro and proved to exhibit strong activity with IC50 value equals to 13.3 ± 5.0 ppm and IC90 value equals to 21.5 ± 8.0 ppm. Conclusion: Two new saponins were characterized from their mixture and found to exhibit α-glucosidase inhibitory activity.

10aHarpullia pendula10aSapindaceae10aTriterpenoid saponins1 aNabil, Marian1 aGhaly, Neveen, S1 aKassem, Iman, AA1 aGrace, Mary, H1 aMelek, Farouk, R uhttps://phcogj.com/article/1030