02710nas a2200205 4500008004100000245013700041210006900178260001900247300001200266490000700278520204600285653002602331653003502357653001702392653000802409653001902417100001802436700001402454856003602468 2020 eng d00aChemical Fingerprint by HPLC-DAD-ESI-MS, GC-MS Analysis and Anti-Oxidant Activity of Manasamitra Vatakam: A Herbomineral Formulation0 aChemical Fingerprint by HPLCDADESIMS GCMS Analysis and AntiOxida cFebruary 2020 a115-1230 v123 a

Background: Manasamitra Vatakam is a classical ayurvedic herbo mineral formulation used for the treatment of neurodegerative properties and epileptic disorders. The wide range mixture of herbal extracts and minerals were used in the formulation. Aim: The aim of the study implies in performing the chemo-profiling, chromatographic fingerprint analysis by HPLC-DAD-ESI-MS for the selected formulations of Manasamitra Vatakam followed by the identification of bioactive compounds by Gas Chromatography – Mass Spectrometric (GC-MS) analysis, to evaluate the diffusion and dilution methods for the determination of anti-bacterial activity in the methanolic extracts of Manasamitra Vatakam (MMV). Materials and Methods: The antibacterial activity was performed by both diffusion and dilution methods whereas the antioxidant activity was performed by free radical scavenging of 2,2-diphenyl-1-picrylhydrazy and hydrogen peroxide scavenging assay method. Results: The estimation of bioactive constituents showed positive results by qualitative analysis. Antibacterial activity of MMV was evaluated against two-gram positive Staphylococcus aureus and Bacillus cereus, two gram negative Escherichia coli and Klebsiella pneumonia by disk diffusion (0.078-10μg mL-1), broth dilution (0.078-10μg mL-1) and broth micro dilution method (0.39-50μg mL-1) respectively. The bioactive constituents were analysed by GC-MS analysis for the methanolic extract of the formulation. Conclusion: To conclude, the formulation was found abundant with phenolic and flavonoid compounds by HPLC-ESI-MS analysis, the bioactive compounds identified are responsible for the anti-bacterial activity. The broth microdilution method performed by resazurin method was observed as the fast screening, sensitive and accurate method for the quantitative determination of antibacterial activity.

10aClassical formulation10aDiffusion and dilution methods10aHeavy metals10aMIC10aPhytochemicals1 aV, Srikalyani1 aK, Ilango uhttps://phcogj.com/article/1066