02872nas a2200253 4500008004100000245017200041210006900213260001900282300001000301490000700311520200600318653002502324653001502349653001302364653002102377653002902398653002102427100002302448700002902471700002702500700002602527700002902553856003602582 2020 eng d00aPotential of Hard Candy Containing Spray-Dried Vernonia cinerea Extract with Total Phenolic Compounds, Total Flavonoids and Nicotine Replacement as an Anti-Smoking Aid0 aPotential of Hard Candy Containing SprayDried Vernonia cinerea E cFebruary 2020 a35-430 v123 a

Backgound: Vernonia cinerea (VC) is a natural plant claimed to reduce cigarette smoking. Some pilot anti-smoking products with nicotine replacement, such as lozenges or gum, have been presented, but with some adverse effects. Thus, application of VC as a new-anti-smoking product is very challenging. Objectives: The aims of this study were to compare the active compounds; total phenolic compounds, total flavonoids and nicotine, and study antioxidant activity on scavenging 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picryl hydrzayl (DPPH) radicals of extracts prepared by spray drying (SD) and freeze drying (FD) techniques for pilot hard candy. Methods: Raw VC materials of mixed parts, i.e., the stem, flowers and leaves, were made to form extracts by FD and SD techniques. Then, extract from the SD technique was manufactured industrially into hard candy containing glucose syrup and refined glucose. Total phenolic compounds, total flavonoids, nicotine, scavenging activity of extracts, VC hard candy and placebo candy were evaluated by folin-ciocalteau reagent, aluminum chloride colorimetric assay, high-performance liquid chromatography, ABTS cation decolorization and DPPH protocols. Results: Total phenolic compounds were significantly different between extracts, but total flavonoids and nicotine were slightly higher in SD extract. Antioxidant activity of both extracts on ABTS radicals was not significantly different, but the half-maximal inhibitory concentration (IC50) on DPPH radicals was significantly higher in SD extract when compared to the FD extract. Finally, total phenolic compounds, total flavonoids and nicotine, as well as scavenging activity could be detected in hard candy. Conclusion: VC can be used as an anti-smoking aid with nicotine replacement and anti-oxidant compounds in pilot hard candy.

10aantioxidant activity10aHard candy10aNicotine10aTotal flavonoids10aTotal phenolic compounds10aVernonia cinerea1 aKanthain, Rungtiwa1 aSinghatong, Supawatchara1 aNatakankitkul, Surapol1 aDechsupa, Nathupakorn1 aLeelarungrayub, Jirakrit uhttps://phcogj.com/article/1071