02618nas a2200241 4500008004100000245019600041210006900237260001900306300001200325490000700337520182100344653001502165653000902180653000802189653002102197653001402218100002202232700001602254700001702270700002502287700002802312856003602340 2020 eng d00aIron-Folate Supplementation during Pregnancy for Prevent Oxidative Stress in Pregnant Rats: Level of MDA, Creatinine, Glucose, Erythrocite, Blood Pressure, Body Weight and Number of Offspring0 aIronFolate Supplementation during Pregnancy for Prevent Oxidativ cFebruary 2020 a186-1910 v123 a

Background: Iron and folic acid deficiency during pregnancy can increase oxidative stress and result in impaired intra-uterine growth, abortion and preeclampsia. Folate is trace nutrient that influent for essential role for epigenetic mechanism cues into changes in gene expression and had impact health development. This study aimed to determine the effect of several doses of iron and folate supplementation on level of: MDA, glucose, creatinine, maternal body weight and number and birth weight of offspring. Methods: This research was conducted in the laboratory of the Center for Food and Nutrition Studies, Gadjah Mada University Yogyakarta. Experimental research with posttest only control group design with a number of samples: 20 pregnant rats, divided randomly into 4 groups. The control group (C) was given standard feed (AIN-93G), KI: added iron 1,8 mg/200gBW and folic acid 0,0023mg/200gBW, KII: added iron 3,6 mg/200gBB and folic acid 0,0045 mg/200gBW, KIII : added iron 5,4mg/200gBW and folic acid 0,0068 mg/200gBW. Duration of treatment 20 days. Measurement of body weight, blood pressure and then taken blood samples at the 21st day for examination of MDA, glucose, creatinine, erythrocyte level. Sectio caesarean to performed the number and body weight of offspring. Data obtained were analyzed using one way Anova followed by Post hoc LSD. Results: there are significant different (p <0.001). on level of : MDA, glucose, creatinine, maternal body weight, average number and fetal weight of offspring between treatment group compare to control group. Conclusion: Iron and folate suplementation during pregnancy can decreased level of oxidative stress and better pregnant outcome.

10aFolic acid10aIron10aMDA10aOxidative stress10aPregnancy1 aWahyuwibowo, Joko1 aAziz, Abdul1 aSafitri, Eka1 aFasitasari, Minidian1 aZulaikhah, Siti, Thomas uhttps://phcogj.com/article/1087