02440nas a2200265 4500008004100000245011500041210006900156260001900225300001000244490000700254520161900261653002401880653002001904653001101924653003301935653001201968653000801980100002101988700002702009700001802036700003302054700002902087700002202116856003602138 2020 eng d00aThin Layer Chromatography Fingerprinting and Clustering of Orthosiphon stamineus Benth. from Different Origins0 aThin Layer Chromatography Fingerprinting and Clustering of Ortho cFebruary 2020 a79-870 v123 a

Introduction: Orthosiphon stamineus has been widely used across Asian countries for the treatment of various diseases. The quality of herbal medicine determine its safety as well as efficacy; and geographical origin is important factor contributing on the quality of herb and its products. Materials and Methods: Thin Layer Chromatography (TLC) method combined with chemometric, Principal Component Analysis (PCA), has been employed to evaluate the quality of Orthosiphon stamineus leaves collected from eleven origins in Indonesia. Results: The results showed that mobile phase suitable for Orthosiphon stamineus was chloroform, dichloromethane, ethyl acetate (7:4:1). The method used has met the requirements of TLC system stability and precision. TLC-fingerprints analyzed with chemometrics showed an ability to discriminate Orthosiphon stamineus from various origins. PCA score plot of the first two principal components (PC) clearly distinguished 3 clusters of samples, whereas the loading plot of the first two PC showed that compounds with the Rf values of 0.0-0.1, 0.1-0.2, 0.2-0.3, and 0.9-1.0 are the most important compounds for clustering of samples. Conclusions: TLCfingerprint combined with the PCA was able to discriminate among the leaves of Orthosiphon stamineus originated from various locations. TLC-fingerprints analyzed with chemometrics can be used as an alternative of marker-oriented method to evaluate the quality of Orthosiphon stamineus.

10aGeographical origin10aHerbal medicine10aMarker10aPrincipal Component Analysis10aQuality10aTLC1 aKartini, Kartini1 aDewi, Ervina, Rustiana1 aAchmad, Fandi1 aJayani, Nikmatul, Ikhrom Eka1 aHadiyat, Mochammad, Arbi1 aAvanti, Christina uhttps://phcogj.com/article/1088