03080nas a2200289 4500008004100000245014700041210006900188260001900257300001000276490000700286520215700293653001602450653001702466653001502483653001802498653002502516100001802541700001902559700003502578700002302613700001902636700002902655700002802684700002102712700002102733856003602754 2020 eng d00aPhytochemical Composition and Evaluation of Marine Algal Sargassum polycystum for Antioxidant Activity and In Vitro Cytotoxicity on Hela Cells0 aPhytochemical Composition and Evaluation of Marine Algal Sargass cFebruary 2020 a88-940 v123 a

Introduction: Sargassum polycystum is one of marine algal which has a potent antioxidant anticancer activities. This research aims to investigate phytochemical composition, antioxidant activity and in vitro cytotoxicity of marine algal Sargassum polycystum on cervical HeLa cancer. Methods: Sargassum polycystum collected from Dompu beach, Lombok, Nusa Tenggara Barat Province, Indonesia, were extracted into organic solvent of n-hexane, ethylacetate, chloroform and ethanol, respectively. Subsequently, Sargassum polycystum extracts were applied for Thin Layer Chromatography (TLC) analysis, phytochemistry test, total phenolic and total flavonoid contents, as well as for antioxidant activity test by DPPH (2,2-diphenyl-1-picrylhydrazyl) method, and in vitro cytotoxicity evaluation on HeLa cells by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide) assay. Results: Phytochemical analysis of S. polycystum extracts are positive for metabolites of flavonoid, steroid, tannin and glycoside. TLC analysis revealed that S. polycystum extracts containing four phytochemical components. Ethylacetate extract of S. polycystum showed the highest total phenolic content, and exhibited greater antioxidant activity than ethanol extract. Total phenolic and total flavonoid content in ethylacetate extract are 548.61 μg/mL and 40.06 μg /mL, respectively. Ethylacetate extract of S. polycystum with IC50 value of 298.3 μg/mL is assigned to have a weak antioxidant activity against DPPH free radical. The results indicate that antioxidant activity of ethylacetate extracts of S. polycystum is directly correlated with its total phenolic and flavonoid content. Moreover, S. polycystum extracts demonstrated a strong anticancer activity on cervical HeLa cells with IC50 ranging from 38.3 μg/mL to 112.8 μg/mL. Conclusion: This work confirmed that S.polycystum are promising natural antioxidant and anti-cervical cancer agents.

10aAntioxidant10aCytotoxicity10aHeLa cells10aphytochemisty10aSargassum polycystum1 aArsianti, Ade1 aBahtiar, Anton1 aWangsaputra, Vincent, Kharisma1 aAzizah, Norma, Nur1 aFachri, Wilzar1 aNadapdap, Lince, Dameria1 aFajrin, Ajeng, Megawati1 aTanimoto, Hiroki1 aKakiuchi, Kiyomi uhttps://phcogj.com/article/1091