02397nas a2200217 4500008004100000245013200041210006900173260001200242300001000254490000600264520168600270653001701956653001901973653003001992653001702022653001702039100002802056700002902084700003102113856003502144 2016 eng d00aGC/MS Analysis and Potential Cytotoxic Activity of Haplophyllum tuberculatum Essential Oils Against Lung and Liver Cancer Cells0 aGCMS Analysis and Potential Cytotoxic Activity of Haplophyllum t c09/2015 a66-690 v83 a

Background: Haplophyllum tuberculatum is a plant belongs to family rutacee. It is rich in volatile oils, fixed oils, alkaloids, and furanocoumarins. It is well known for the huge number of folkloric uses in middle east.  Objective: The aim of this study is to establish the chemical composition of the essential oils of Libyan H. tuberculatum and to investigate their cytotoxic potentialities. Materials and Methods: The essential oils of the aerial parts and flowers of H. tuberculatum growing in Libya were prepared by hydrodistillation. GC/MS analyses were performed on a Shimaduz capillary gas chromatograph (GC 17A ver.3) instrument directly coupled to mass spectrometer-MS QP5050A. Oil A and F of H. tuberculatum at different concentrations (0-50 µg/ml) in DMSO were tested for cytotoxicity against human tumor cell lines. Results: Oil yield was found 0.4 and 1.5 (v/w %) on dry weight basis respectively. GC/MS analysis resulted in identification of total 35 compounds. 15 compounds  were common to both oils. oil A of H. tuberculatum exhibited antitumor activities against. liver carcinoma cell line (HEPG2) and lung carcinoma cell line (H1299) 4.7 µg/ml and 4.1 µg/ml. Conclusion: Essential oil of the aerial parts of H. tuberculatum is potentially active against lung (H-1299), and liver (HEPG2) carcinoma cell lines. The observed cytotoxic, activities can be attributed to the dominance of α and γ-terpinene in this oil.

10aCytotoxicity10aEssential oils10aHaplophyllum tuberculatum10aLiver cancer10aLung cancer.1 aSabry, Omar, Mohamed Mo1 aSayed, Abeer, Mohamed El1 aAlshalmani, Salmin, Khalid uhttps://phcogj.com/article/111