02575nas a2200253 4500008004100000245015000041210006900191260001500260300001200275490000700287520180800294653001702102653001602119653001502135653001402150653001602164653001702180100001902197700002902216700001802245700001702263700000502280856003602285 2020 eng d00aAntidiabetic and Antioxidant Effect Combination Vasconcellea pubescens A.DC. and Momordica charantia L. Extract in Alloxan- Induced Diabetic Rats0 aAntidiabetic and Antioxidant Effect Combination Vasconcellea pub cMarch 2020 a311-3150 v123 a

Introduction: Mountain papaya (Vasconcellea pubescens A.DC.) and bitter melon (Momordica charantia L.) fruit extract have total flavonoids and some metabolite from its contains. The use of a single dose in the treatment of diabetes is often considered to lack a strong effect. Giving a combination of the compound is a solution to provide a synergistic effect in treatment. Material and Methods: Rat were divided into eight groups (normal, negative control, glibenclamide as a positive control, and five dose extract group). The single dose mountain papaya extract (MPE) and bitter melon extract (BME) were given at 174 mg/kg b.w of MPE, and 380 mg/kg b.w of BME in oral administration. Combination of MPE:BME (25:75, 50:50, 75:25)% or (87:190; 44:285 and 130:95) mg/kg b.w in oral administration. Induction of diabetes used alloxan dose of 150 mg/kg b.w intraperitoneally. The treatment was performed for 21 days with a frequency of once a day. Blood sugar level was measured at pretest, 7th, 14th, and 21st days. At the end of the test, measurements of malondialdehyde (MDA), glutathione (GSH), and pancreatic morphology were measured. Data were analyzed by ANOVA statistic. Results: The single and combination of MPE-BME showed an decrease in blood glucose levels significantly compared with the negative control (p ≤ 0.05). The same results from measurements of levels of malondialdehyde (MDA), glutathione (GSH), and pancreatic morphology. Conclusions: Based on the research, a combination of the MPE-BME had antidiabetic and antioxidant activity, but the activity was not significantly different from both single-dose extract (p> 0.05).

10aAntidiabetic10aAntioxidant10aFlavonoids10aMomordica10aSynergistic10aVasconcellea1 aSasongko, Heru1 aLestari, Ratih, Guswinda1 aYugatama, Adi1 aFarida, Yeni1 a uhttps://phcogj.com/article/1110