02254nas a2200229 4500008004100000245009900041210006900140260001500209300001200224490000700236520155900243653001801802653002001820653001601840653002901856100001901885700002801904700002101932700001901953700001601972856003601988 2020 eng d00aAnti-Elastase, Anti-Tyrosinase and Anti-Oxidant of Rubus Fraxinifolius Stem Methanolic Extract0 aAntiElastase AntiTyrosinase and AntiOxidant of Rubus Fraxinifoli cMarch 2020 a271-2750 v123 a

Introduction: Some Rubus were reported had anti-skin aging activity. Rubus fraxinifolius was one of Rubus genus which lives in Indonesian highland. Objective: This study was to examine elastase, tyrosinase, and oxidant inhibitory activity of R. fraxinifolius stem (RFS) extract. Methods: Extraction was done by a Soxhlet apparatus using methanol as solvent. Elastase inhibition activity was determined, which based on the formation of p-nitroaniline. Tyrosinase inhibition activity evaluated based on inhibition of mushroom tyrosinase by the sample with L-DOPA as substrate. The activity of antioxidant was determined using the DPPH radical scavenger method. LC-MS was used for prediction of naturally occurring phytochemicals. Results: The RFS extract yield was 9.03 %. The RFS extract revealed inhibition activity against elastase and tyrosinase with IC50 128.85 ppm, and 155.19 ppm, respectively. DPPH radical scavenging activity gave IC50 63.04 ppm. Total phenolic content of the extract was 387.99+3.21 mg GAE/g extract. The LC-MS analysis showed the presence of at least 13 different organic compounds in RFS extract, which might contribute to the bioactivity. Conclusion: Therefore, this experiment further proved that RFS extract might be useful as a natural product ingredient of anti-photoaging skincare products because of its ability to inhibit elastase, tyrosinase, and as an antioxidant.

10aAnti-Elastase10aAnti-tyrosinase10aAntioxidant10aRubus fraxinifolius stem1 aDesmiaty, Yesi1 aSaputri, Fadlina, Chany1 aHanafi, Muhammad1 aPrastiwi, Rini1 aElya, Berna uhttps://phcogj.com/article/1115