02523nas a2200205 4500008004100000245008400041210006900125260001500194300001200209490000700221520193900228653001302167653001002180653001502190653000802205100002402213700002402237700002002261856003602281 2020 eng d00aPhytochemical Compounds in Arundo donax L. Rhizome and Antimicrobial Activities0 aPhytochemical Compounds in Arundo donax L Rhizome and Antimicrob cMarch 2020 a287-2920 v123 a

Introduction: The aerial part of Arundo donax L., giant reed, is a well-known fuel source used in many countries. Methods: Phytochemical compounds in A. donax L. rhizome, sequentially extracted with hexane (HEX), dichloromethane (DCM), ethyl acetate (EA), and methanol (MeOH), were identified using gas chromatography-mass spectrometry. Antimicrobial activities of the rhizome extracts were evaluated using disc diffusion assay against yeast (Candida albicans), and bacteria Gram-positive (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC11778, and Bacillus subtilis ATCC6633) and Gram-negative (Escherichia coli ATCC25922). Results: The detected phytochemicals were screened against WILEY07 library; 84 compounds matched with a similarity ≥ 90%. All the characterized compounds were grouped based on their functional group. The major phytochemicals in the HEX, DCM, and EA extracts belonged to sterol groups, while lipids, fatty acids, and related conjugates were the main components of the methanolic extract. The other characterized compounds were hydrocarbons, phenolics, terpenoids, xanthones, and xanthene. Growth of B. subtilis was inhibited by the HEX, DCM, EA, and MeOH extracts, whereas B. cereus growth was inhibited only by the DCM and EA extracts. However, growth of E. coli and C. albicans could be not inhibited by A. donax L. rhizome extracts. Analysis of the compounds as well as their antibacterial activities via hierarchical clustering showed that hexadecanoic acid is the major compound influencing B. subtilis growth, while, B. cereus growth was affected by xanthone. Conclusions: Rhizomes of A. donax L. is one potential source of antimicrobial agents and further applied in medicinal uses.

10aBacteria10aGC-MS10aGiant reed10aHCA1 aPansuksan, Kanoktip1 aSukprasert, Sophida1 aKaraket, Netiya uhttps://phcogj.com/article/1117