02284nas a2200205 4500008004100000245010700041210006900148260001500217300001200232490000700244520163800251653001201889653001601901653002701917653001401944100002501958700002801983700003102011856003602042 2020 eng d00aHigh Dose Allicin with Vitamin C Improves EPCs Migration from the Patient with Coronary Artery Disease0 aHigh Dose Allicin with Vitamin C Improves EPCs Migration from th cMarch 2020 a232-2350 v123 a

Endothelial Progenitor Cells (EPCs) have an important role in endothelial dysfunction repairment through neovasculogenesis and cardiac myocytes regeneration. However, EPCs migration is greatly reduced in the patient with Coronary Artery Disease (CAD). Allicin and Vitamin C are hypothesized to improve EPCs migration due to its antioxidant properties. Objective: To investigate the effect of Allicin and its combination with Vitamin C in EPCs migration of CAD patients. Material and Method: Mononuclear cells were isolated from CAD patients and cultured on fibronectin-coated plates with colony-forming unit Hill medium. The cells were divided into untreated (control), Allicin treatment (dose 100 mcg/ml, 200 mcg/ml, 400 mcg/ ml), and each dose of Allicin combined with 250 mcg/mL of Vitamin C. EPCs migration was assessed with Transwell Migration Assay Kit and evaluated by using statistical tests. Results: This research shows that EPC migration was significantly higher in the treatment. Allicin at all dose (dose 100 mcg/ml, 200 mcg/ml, 400 mcg/ml) and its combination with 250 mcg/mL of vitamin C compared to untreated group (p<0.05). Allicin increase EPCs migration in a dosedependent manner. However, the only combination of 400 mcg/ml Allicin with 250 mcg/mL of vitamin C which has significantly higher EPCs migration compared to Allicin treatment alone. Conclusion: Allicin improves EPCs migration in a dose-dependent manner. Improvement of the migration only observed on the Allicin dose 400 mcg/ml with Vitamin C.

10aAllicin10aAntioxidant10aEndothelial Progenitor10aMigration1 aOktaviono, Yudi, Her1 aAmadis, Muhammad, Rafdi1 aAl-Farabi, Makhyan, Jibril uhttps://phcogj.com/article/1121