02684nas a2200229 4500008004100000245022700041210006900268260001300337300001300350490000700363520187500370653001402245653001202259653001802271653001302289100002202302700002202324700002602346700001802372700002802390856003602418 2020 eng d00aEffect of Phaleria macrocapa on Atrophy and Apoptosis of Intestinal Mucous Cell and Phalerin Concentration at Portal Vein and Systemic Circulation in Adenocarcinoma Mice following Adriamycine and Cyclophosphamide Treatment0 aEffect of Phaleria macrocapa on Atrophy and Apoptosis of Intesti cMay 2020 a603-610 0 v123 a

Introduction: Chemotherapy has been proven capable of reducing breast cancer cell progression; however the adverse effect also emerging. Thus, diminish those adverse effects with botanical product Phaleria macrocarpa (PM) as adjuvant therapy is necessary. Objective: This study aimed to evaluate the effect of PM treatment in combination with adriamycine and cyclophosphamide (AC) on intestinal apoptosis and their correlation with phalerin concentration in systemic circulation. Methods: In the experimental study, 30 female mice with adenocarcinoma were assign into 5 groups: Neg-G, only given aquadest; Portal vein group (PMV-G) and systemic circulation groups (PMC-G), were administered PM 0.146mg/day; Portal vein group (PMACV-G) and systemic circulation group (PMACC-G), were administered Phaleria macrocarpa 0,146 mg orally, Adriamycine 0,013 mg and Cyclophosphamide 0,0156 mg singgle dose intravenously. Phalerin concentration was measured by HPLC methods at minute 30, 60, 90, 120, 150, and 180 after treatment. At the end of study, intestinal mucous cell apoptosis was identified by TUNEL methods. Results: independent t test analyses showed that index of apoptosis of intestinal mucous cell were significant higher in PMAC-G compared to that of Neg-G and PM-G, p < 0.05. In contrary, phalerin concentration in PMAC-G was significant lower compared to that of PM-G, p < 0.05. The Pearson analysis indicated the inverse correlation (r= -736, p>0.05) between apoptosis index with phalerin concentration. Conclusion: Treatment of PM in combination with AC has been proven able to increase intestinal mucous cell apoptosis and decrease phalerin concentration. However, the inverse correlation didnot exist.

10aApoptosis10aAtrophy10aConcentration10aPhalerin1 aSumarawati, Titik1 aRiwanto, Ignatius1 aHadisaputro, Soeharyo1 aDharmana, Edi1 aNasihun, Taufiqurachman uhttps://phcogj.com/article/1151