02921nas a2200313 4500008004100000245012200041210006900163260001400232300001200246490000700258520191700265653002802182653001702210653002202227653000902249653001502258100003102273700002902304700003102333700002602364700003102390700002102421700001902442700003102461700003202492700002102524700002602545856003602571 2020 eng d00aAntimalarial Effects of the Aqueous Extract of Entandrophragma angolense Bark on Plasmodium berghei Infection in Mice0 aAntimalarial Effects of the Aqueous Extract of Entandrophragma a cJune 2020 a687-6980 v123 a

Background: Research for new antimalarial drugs remains a permanent quest for the control of malaria. Objective: The present study investigates the effects of the aqueous extract of Entandrophragma angolense bark on P. berghei-induced malaria in mice. Methods: Eight weeks old mice, were intraperitoneally infested with 200 μl of blood, containing 1x106 P. berghei-infected-erythrocytes. Parasitaemia was determined using a 10% giemsa stained blood smear read under optical microscope (x100). The infected animals were randomized into 5 groups of 10 animals each and daily treated for 5 days with the plant extract at 125, 250 and 500 mg/kg. The normal control and malaria control received water while the chloroquine control was treated with 10 mg/kg of chloroquine. Body weight, parasitaemia and survival time were monitored daily during treatment and follow up periods. Five animals from each group were sacrificed under anaesthesia at the end of treatment (d8) and after the follow up period (d28). Venous blood was used for haematological and biochemical tests. Organs (liver, kidneys and spleen) were also collected for biochemical and histological analyses. Results: Administration of the aqueous extract of E. angolense bark to infected mice significantly inhibited parasite development (p <0.001) with ED50 estimated at 25.32 mg/kg. The extract prevented animal from death, body weight loss, anaemia, leucocytosis, high transaminases (ALT and AST), high bilirubin, creatinine and MDA levels, oxidative stress and anatomical alteration in organs as compared to the malaria control. Conclusion: The E. angolense bark possesses antimalarial properties, supporting its use in traditional medicine to treat malaria.

10aAntiplasmodial activity10aE. angolense10aMalaria infection10aMice10aP. berghei1 aKamkumo, Raceline, Gounoue1 aBetene, Abel, Narcisse M1 aFokou, Patrick, Valere Tso1 aDonfack, Jean, Hubert1 aNangap, Marius, Jaurès Ts1 aNgako, Albertine1 aFokou, Roberto1 aTali, Mariscal, Brice Tcha1 aTsofack, Florence, Ngueguim1 aDimo, Théophile1 aBoyom, Fabrice, Fekam uhttps://phcogj.com/article/1165