01925nas a2200277 4500008004100000245012700041210006900168260001400237300001200251490000700263520107200270653001601342653001901358653002001377653001801397653000901415100001801424700001801442700002801460700001901488700002801507700002401535700002401559700002801583856003601611 2020 eng d00aIsolation, Identification Similarity and Qualitative Expression of Metallothionein Gene in IR-Bagendit Rice (Oryza sativa)0 aIsolation Identification Similarity and Qualitative Expression o cJune 2020 a709-7150 v123 a
Metallothionein (MTs) is an enzyme that plays a role in the binding of metals in plants. Various types of rice have been known to contain MTs and IR-Bagendit rice leaves have the highest MTs protein content compared to other rice varieties. However, MTs coding gene in IRBagendit rice variety is still unknown. OsRAC1 gene is reported as the down-regulator of MTs and there is an analogous gene for MTs-like gene using RAP1 and RAP2 primers in various plants. This study aimed to isolate, identification of similarity, and analysis of qualitative expression of MTs gene in IR-Bagendit rice as compared to Inpari, IR-36, and IR-34. The steps of this research were DNA isolation, PCR in OsRAC1 gene, RNA isolation and cDNA reverse transcription using primer RP1 and RP2, and agarose gel electrophoresis. Amplification quality of OsRAC1 gene in IR-Bagendit, Inpari, IR-36, and IR-34 showed the same result. Qualitative expression of MTs by reverse transcription showed that IR-Bagendit has the highest MTs-like gene compared to other samples.
10aIR-Bagendit10aMetal Exposure10aMetallothionein10aMTs-like gene10aRice1 aSantosa, Budi1 aSantosa, Budi1 aKartika, Aprilia, Indah1 aNuroini, Fitri1 aErnanto, Aditya, Rahman1 aAyuningtyas, Annisa1 aSalleh, Mohd, Nazil1 aZulaikhah, Siti, Thomas uhttps://phcogj.com/article/1167