02424nas a2200229 4500008004100000245011100041210006900152260001400221300001200235490000700247520178300254653002002037653001002057653001502067653001502082653001002097100002502107700000502132700000502137700001602142856003602158 2020 eng d00aAnti-Inflammation of Soursop Leaves (Annona muricata L.) Against Hemorrhoids in Mice Induced by Croton Oil0 aAntiInflammation of Soursop Leaves Annona muricata L Against Hem cJune 2020 a784-7920 v123 a

Background: Hemorrhoids are rectoanal venous plexus swelling that causes inflammation, pain, and bleeding. Plants with phenolic compounds are known to improve venous tone and anti-inflammation. Soursop leaves (Annona muricata L.) known contain phenolic compounds and have been used to cure inflammation. However, studies on anti-inflammatory soursop leaves for hemorrhoids are still limited. Objective: This study aims to analyze the effect of Soursop Leaves Ethanol Extract (SLEE) on the histopathological features and expression of COX-2 and TNFα in rectoanal tissue. Methods: Swiss mice 20 weeks induced 3 times with 6% croton oil through the anus. SLEE doses of 100, 200, and 400 mg/Kg and aspirin as a positive control were given orally for 7 days. Histopathological examination of the rectoanal tissue of mice was assessed by counting cell necrosis, inflammation, vasodilation, and edema using hematoxylin-eosin. Positive cells expressing COX-2 and TNFα were counted on inflammatory epithelial cells using immunohistochemistry. Results: Administration of SLEE at all doses showed different levels of inflammation, necrosis, vasodilatation and edema in histopathology of rectoanal tissue P <0.00. All three doses of SLEE show significant anti-inflammatory effects on hemorrhoidal tissue. SLEE doses of 200, 400 mg/Kg significantly decreased COX-2 P <0.05 compared to negative controls, and SLEE doses of 100, 200, and 400 mg/Kg significantly decreased TNFα P <0.05 compared to negative controls. Conclusions: SLEE can reduce inflammation and has the potential to be developed as a natural remedy for hemorrhoids.

10aAnnona muricata10aCOX-210aCroton oil10aHemorrhoid10aTNFα1 aAyun, Nurul, Qurrota1 a1 a1 aElya, Berna uhttps://phcogj.com/article/1176