02807nas a2200289 4500008004100000245014600041210006900187260001400256300001200270490000700282520185700289653002102146653001602167653002602183653001402209653002102223653002002244100002502264700002302289700002502312700003002337700002902367700002402396700002802420700003302448856003602481 2020 eng d00aProtection of Erythrocytes against Lipoperoxidation and Antiinflammatory Effects of Ethanolic Extract of Encelia canescens Lam Leaves in Mice0 aProtection of Erythrocytes against Lipoperoxidation and Antiinfl cJune 2020 a798-8040 v123 a

Background: Encelia canescens Lam is a plant traditionally used in Peru for medicinal purposes, and is attributed antioxidant properties, indicating that it could be used in the prevention of non-communicable diseases. Objective: This study aims to evaluate the protection of erythrocytes from lipoperoxidation and the anti-inflammatory effect of ethanolic extract of E. canescens leaves in mice. Materials and methods: Protection from lipoperoxidation was evaluated by inhibition of hemolysis and quantifying malondialdehyde (MDA) concentration against oxidative stress induced with hydrogen peroxide (H2O2) at 200, 150, 100, 50 and 25 μg/mL E. canescens concentrations. The 1% carrageenan-induced air pouch model was used for evaluated inflammation, where albumin, total proteins, MDA, number and leukocyte differentiation were determined in the exudate, and a histopathological evaluation was performed. The concentrations evaluated were 100, 250 and 500 mg/kg of E. canescens Results: All the concentrations evaluated protected protected erythrocytes from lipoperoxidation (p<0.05), being E.D. value 200 μg/mL. Regarding anti-inflammatory effect, the albumin, total proteins and MDA values of the treatment groups were lower than carrageenan 1% group (p<0.05), but, due to less leukocyte migration and presence of macrophages and the histopathological evaluation, the E.D value was 500 mg/kg. Conclusion: Ethanolic extracts of E. canescens leaves protect erythrocytes from lipoperoxidation and have dose-dependent anti-inflammatory effects maybe for presence of p-hydroxyacetophenone-derived, and these could be new safer anti-inflammatories.

10aAntiinflammatory10aCarrageenan10aEncelia canescens Lam10aHemolysis10aLipoperoxidation10aMalondialdehyde1 aN, Fernández-Flores1 aNF, Rojas-Cardenas1 aAD, Vásquez-Quispe1 aE, Chávez-Flores, Juana1 aJ, Justil-Guerrero, Hugo1 aJM, Parreño-Tipian1 aR, Silva-Correa, Carmen1 aE, Villarreal-La, Torre Víc uhttps://phcogj.com/article/1177