02009nas a2200217 4500008004100000245010300041210006900144260001600213300001400229490000700243520133200250653001901582653001501601653001401616653002401630100002301654700002201677700003001699700002601729856003601755 2012 eng d00aEvaluation of Anticancer Potential of Vitus vinifera Seed Against Breast Cancer Cells - MDA-MB-2310 aEvaluation of Anticancer Potential of Vitus vinifera Seed Agains cAugust 2020 a1064-10710 v123 a

Objective: The aim of the present research is to evaluate the anti-cancer effect of Vitus vinifera seed on MDA-MB-231 cell line. Methods: The Vitus vinifera (Grape) seed were dried, powdered and subjected to methanol, chloroform and ethyl acetate extraction by cold maceration followed by preliminary phytochemical screening. The extracts of Vitus vinifera seed were subjected to assess anti-oxidant status, anti-proliferative activity by MTT assay, GC-MS analysis and apoptotic effect by determining LDH activity on MDA-MB-231. Results: Results indicated that methanolic extract of grape seed showed appreciable anti-oxidant and anti-cancer potential compared with other two extracts. GC-MS mass spectrum of methanolic extract of seed revealed the presence of Dotriacontane, Linoleic acid and Decanoic acid ethyl ester, 1,2,3, propenetriol, monocetate, and Dichloro methyl propane sulfone were detected. Conclusion: The data obtained in this work could be useful as a chemical standard in checking the genuineness of this plant source. Data of the results further depicted that the selected traditional Vitus vinifera seed could be used not only as a potential anti-cancer and good antioxidant.

10aGC-MS analysis10aMDA-MB-23110aMTT Assay10aVitus vinifera seed1 aDhanraj, Kiruthika1 aSaravanan, Renuka1 aHameed, Sheik, Abdulla Sh1 aRamalingam, Sivakumar uhttps://phcogj.com/article/1200