02363nas a2200193 4500008004100000245010000041210006900141260001900210300001400229490000700243520177500250653001502025653002102040653000902061653002302070653000802093100003202101856003602133 2020 eng d00aThe Effect of Carthamus tenuis Extracts on the Cell Proliferation of Different Tumor Cell Lines0 aEffect of Carthamus tenuis Extracts on the Cell Proliferation of cSeptember 2020 a1332-13390 v123 a

Background: Carthamus tenuis is one of the medicinal plants that was used traditionally to treat skin diseases, hemorrhoids, abortion, infertility. It also showed an immunosuppressive role as well as antifungal, antibacterial, anti-inflammatory activity. Although this plant is widespread, there are few studies about its medical applications. Objectives: This study was done to explore the anticancer activity of this plant. Materials and Methods: The aerial parts of the plant were dried, grinded and extracted with hexane, ethyl acetate, and methanol. The extracts were applied in different concentrations to cell cultures of breast (MCF-7), colon (HT-29), prostate (PC-3) and colorectal (CaCo-2) cell lines and fibroblast (MRC- 5) was used as a control. The anticancer activity was evaluated by 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium (MTT) reduction assay that was measured by spectrophotometer. Results: The results showed that methanol extract significantly (p<0.05) have the highest inhibitory activity on MCF-7, HT-29, PC-3, and CaCo-2 with IC50; (25.52 μg/ml), (17.37 μg/ml), (25.77 μg/ml), (24.49 μg/ml), respectively. Followed by ethyl acetate extract that moderately inhibit cell growth of PC-3 and CaCo-2 with IC50; (28.99 μg/ml) and (21.45 μg/ml), respectively. n-hexane extract showed no significant inhibitory effect on all cell lines; IC50 (125.52 -152.34 μg/ml) when compared to Tamoxifen drug activity as a positive control. Conclusion: Results of this study showed the anticancer activity of the plant extracts in four different kinds of cancers that need further study.

10aAnticancer10aCarthamus tenuis10aIC5010aMethanolic extract10aMTT1 aShawagfeh, Maissa’, Taleb uhttps://phcogj.com/article/1249