02774nas a2200229 4500008004100000245012600041210006900167260001800236300001400254490000700268520202800275653002102303653002002324653000902344653002402353653003102377100001902408700003002427700002402457700002702481856003602508 2020 eng d00aAntihypertensive Assay-Guided Fractionation of Syzygium polyanthum Leaves and Phenolics Profile Analysis Using LCQTOF/ MS0 aAntihypertensive AssayGuided Fractionation of Syzygium polyanthu cNovember 2020 a1670-16920 v123 a

Introduction: Syzygium polyanthum leaves extract that contains gallic acid as the major phenolic compound has shown significant antihypertensive effect, however the amount of gallic acid was inversely-related with magnitude of this effect. This study aimed to conduct bioassay-guided fractionation of S. polyanthum leaves with gallic acid as a reference compound, and to screen for other possible compounds responsible for the antihypertensive effect. Methods: S. polyanthum leaves were extracted using n-hexane, ethyl acetate, methanol, and water. The most active crude extract was fractionated using column chromatography and analyzed for total phenolic content (TPC) (n=3). Crude extracts and the derived fractions were intravenously administered into pentobarbital-anaesthetized Spontaneously Hypertensive rats (n=5) for recording of blood pressure parameters. Liquid Chromatography-Quadruple Time-Off-Flight/Mass Spectrometry was used for determination of chemical composition. One-way and two-way ANOVA were used for statistical analysis using GraphPad® PRISM Version 6. Results: Fractionation of aqueous S. polyanthum leaves extract (ASP) afforded nine fractions, later combined into three fractions (F1ASP, F2ASP, and F3ASP) based on the thinlayer chromatography profiles. ASP has the highest TPC while F2ASP has the lowest TPC. All fractions exhibited significant antihypertensive property, but F2ASP was the most active fraction. Few phenolics with related antihypertensive effects such as 1-galloyl glucose (a gallic acid-derivative majorly found in F2ASP and F3ASP), and other compounds such as polydatin, sesamol, brazilin, eugenol, ellagic acid, kukoamine A, and cyclocurcumin were found across all active fractions. Conclusion: These phenolics may partly contribute to the antihypertensive effect of S. polyanthum leaves, thus further isolation study is recommended.

10aAntihypertensive10aBioassay-guided10aLCMS10aSyzygium polyanthum10aTotal phenol content (TPC)1 aIsmail, Azlini1 aRahim, Erlena, Nor Asmira1 aOmar, Muhammad, Nor1 aAhmad, Wan, Amir Nizam uhttps://phcogj.com/article/1294