02596nas a2200229 4500008004100000245010500041210006900146260001800215300001400233490000700247520188500254653003702139653002302176653001402199653001702213653002702230653001802257100001802275700001602293700002102309856003602330 2020 eng d00aIsolation and Identification of Chemical Compounds from Garcinia fruticosa Lauterb Stem Bark Extract0 aIsolation and Identification of Chemical Compounds from Garcinia cNovember 2020 a1641-16520 v123 a

Background: Garcinia is a tropical plant that grows in Indonesia. Garcinia has many health benefits for the body. Garcinia contains many phenolic compounds and their derivatives, such as xanthon, flavonoids, benzophenone, lactone, and phenolic acids. Garcinia fruticosa Lauterb. comes from the family Clusiaceae. The results of the phytochemical examination showed that G. fruticosa bark extract contained alkaloids, flavonoids, glycosides, tannins, and saponins. Objective: This study aims to isolate and identify chemical compounds from the ethyl acetate extract of G. fruticosa Lauterb stem bark. Method: G. fruticosa Lauterb bark. dried, milled, and extracted with Step Gradient Polarity/SGP maceration using n-hexane, ethyl acetate, and methanol. Isolation was done by column chromatography and identified by thin layer chromatography and IR spectroscopy, LC-MS/MS, 1H-NMR, 13C-NMR, 2D-NMR (HSQC, HMBC). Results: Compound D7a has a molecular weight 168.0496. The IR spectrum shows the presence of a group –OH appears on 3483 cm-1, aromatic presence in 1609 cm-1. The H-NMR spectrum shows the presence of aromatic signals on 6.96 (d, 8 Hz), 6.96 (d, 2 Hz) and 7.70 (dd, 8; 2 Hz). The C-NMR spectrum shows the presence of a carboxylic-COOH group appearing at 166.57 ppm, the presence of 2 x C-OH appearing at 147.18 and 151.18. In the HMBC spectrum, the -OCH3 position is located at C-3 with a correlation between the 3.79 (s) signal and the C signal at the chemical shift 147.18. Conclusions: Structural elucidation shows that compound D7a is a 4-hydroxy-3-methoxy benzoate acid (Vanylic Acid) and isolate I-1 is an impure compound namely β-Sitosterol and Stigmasterol.

10a4-hydroxy-3-methoxy benzoic acid10aGarcinia fruticosa10aIsolation10aStigmasterol10aStructural elucidation10aβ-sitosterol1 aDelita, Novia1 aElya, Berna1 aHanafi, Muhammad uhttps://phcogj.com/article/1316