02434nas a2200241 4500008004100000245016400041210006900205260001700274300001000291490000700301520165000308653001401958653001701972653001701989653002302006100002302029700001902052700002202071700001902093700002802112700001602140856003602156 2021 eng d00aPotential of Phaleria macrocarpa Leaves Ethanol Extract to Upregulate the Expression of Caspase-3 in Mouse Distal Colon after Dextran Sodium Sulphate Induction0 aPotential of Phaleria macrocarpa Leaves Ethanol Extract to Upreg cJanuary 2021 a23-290 v133 a

Ulcerative colitis (UC) is a part of incurable chronic inflammatory disease that has gained importance over the past few decades. A lot of research has been done to find effective treatments for UC, one of which is herbal medicine. Phaleria macrocarpa (PM), an Indonesian native plant, is thought to be an alternative therapy for UC because of its anti-inflammatory properties. Therefore, in this research, Phaleria macrocarpa Leaves Ethanol Extract (PMLEE) is used to assess its effect on UC by using Caspase-3 as apoptosis marker. PMLEE was made from dried material of PM that undergo maceration. Animals were separated into six groups: normal, negative control, positive control, and PMLEE groups (100, 200, 300 mg/kgBW). PMLEE was then injected to BALB/c mice that have been induced by dextran sodium sulphate (DSS) for 7 consecutive days. DSS is used to model UC in mice colon tissue. All animals were sacrificed and their colons were collected then stained with anti-Caspase-3. The stained sections were subsequently examined with ImageJ based on color intensity which generated H-Score as the results. Based on H-Score of each group, PMLEE 300mg has significantly upregulate the expression of Caspase-3 compare to the negative control (p=0.015). PMLEE also has a tendency to be dose dependent based on the significant difference between PMLEE doses. Therefore, it concludes that PMLEE is able to upregulate the expression of Caspase-3 in colon cells as in this study it was directly proportional. Key words: Mahkota Dewa, Inflammation, Apoptosis, Ulcerative colitis.

10aApoptosis10aInflammation10aMahkota Dewa10aUlcerative colitis1 aKusmardi, Kusmardi1 aWiyarta, Elvan1 aEstuningtyas, Ari1 aSahar, Nurhuda1 aMidoen, Yurnadi, Hanafi1 aTedjo, Aryo uhttps://phcogj.com/article/1326