01936nas a2200217 4500008004100000245013200041210006900173260001700242300001200259490000700271520125400278653002301532653001601555653002801571653001101599653001001610100002501620700001601645700002101661856003601682 2021 eng d00aAntioxidant and Cytotoxic Bioassay on Blumeodendron toxbrai (Blume.) Stem Bark Hexane, Dichloromethane, and Methanolic Ekstract0 aAntioxidant and Cytotoxic Bioassay on Blumeodendron toxbrai Blum cJanuary 2021 a139-1410 v133 a

Introduction: Blumeodendron toksbraii has the potential to be anti-HIV and anti α-glucosidase. Objective: This research was conducted to examine the effects of antioxidant and cytotoxicity in vitro from these compounds from methanolic stem bark extract. Method: Stem bark to be extracted with maceration using hexane, dichloromethane, and methanol solution. Extracts were quantified with respect to in vitro antioxidant activity using the 2.2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging. Anticytotoxic activity was determined by cytotoxicity assay using MCF-7 cell line with Alamar Blue method. Results: The observed IC50 value from hexane, dichloromethane, and methanol extract for antioxidant assay were 88.33 ± 0.19 μg/ mL, 74,54 ± 0,61 μg /mL and 94.1 ± 0.19 μg/mL respectively. IC50 value of anti-cytotoxic assay from hexane extract, dichloromethane and methanol extract is 121.24 ± 0.15 μg/mL, 55 ± 0,48 μg/mL and 70.71 ± 0.15 μg/mL. Conclusion: dichloromethane extract showed good promising result for anti-oxidant and cytotoxic assay, futher study needed to isolate compound from this plant.

10aAnticytotoxic DPPH10aAntioxidant10aBlumeodendron toksbraii10aCancer10aMCF-71 aAdi, Andreas, Susilo1 aElya, Berna1 aHanafi, Muhammad uhttps://phcogj.com/article/1342