02316nas a2200217 4500008004100000245017000041210006900211260001500280300001200295490000700307520159500314653001601909653001601925653002101941653001401962653002501976100002002001700002002021700002102041856003602062 2021 eng d00aProduction of Secondary Metabolites and its Antibacterial and Antioxidant Activity During the Growth Period of Endophytic Fungi Isolated from Gall Rust Sengon Plants0 aProduction of Secondary Metabolites and its Antibacterial and An cMarch 2021 a325-3310 v133 a

Introduction: In our previous study, secondary metabolites of endophytic fungi isolated from gall rust sengon showed their bioactive activity against antibacterial and antioxidant, but only gave little effect to antifungal activity. Endophytic fungal culture extracted from gall rust of sengon (Falcataria moluccana Miq. Barneby and J. W. Grimes) has the ability on inhibiting Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. To increase the production of bioactive compounds, this research was focused on isolates which produced the highest activity compounds, and sought the optimal fermentation conditions in the production of bioactive compounds. Methods: The fermentation process was carried out on PDB liquid media for 21 days, under shaker conditions, at room temperature, and sampling measurements were hold every 3 days. Bioassay were carried out against B. subtilis, P. aeruginosa, and E. coli. Results: The early stages fungal growth was adaptation stage, continued by 1-6th days of exponential growth period. Day 6-12th was stationary growth and day 15-21th were cell death periode. The highest secondary metabolite production was achieved at stationary periodes, that was occured at days 9-15th and high antibacterial activity was produced on days 9-12th. Conclusion: The best production secondary metabolite that has high activity was at day 9-12th.

10aAntibacteri10aAntioxidant10aEndophytic fungi10aGall rust10aSecondary metabolite1 aRumidatul, Alfi1 aRahmawati, Noor1 aSunarya, Sopandi uhttps://phcogj.com/article/1366